Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity
IntroductionOral squamous cell carcinoma (OSCC) is often preceded by oral epithelial dysplasia (OED). The role of ribosomal protein S6 (RPS6) and programmed cell death ligand-1 (PD-L1) in the progression of OED to OSCC remains unclear. This study aimed to investigate the expression of phosphorylated...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Oral Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/froh.2024.1337582/full |
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| author | Jaruwat Hanroongsri Panomwat Amornphimoltham Rania H. Younis Risa Chaisuparat Risa Chaisuparat |
| author_facet | Jaruwat Hanroongsri Panomwat Amornphimoltham Rania H. Younis Risa Chaisuparat Risa Chaisuparat |
| author_sort | Jaruwat Hanroongsri |
| collection | DOAJ |
| description | IntroductionOral squamous cell carcinoma (OSCC) is often preceded by oral epithelial dysplasia (OED). The role of ribosomal protein S6 (RPS6) and programmed cell death ligand-1 (PD-L1) in the progression of OED to OSCC remains unclear. This study aimed to investigate the expression of phosphorylated RPS6 (p-RPS6) and PD-L1 in OSCC and OED and to examine its relationship with clinicopathological features.MethodsFifty-two OSCC and 48 OED cases were recruited for immunohistochemical analysis of p-RPS6 and PD-L1 expression. The expression of markers was correlated with clinicopathological features of OSCC and OED.ResultsWe found p-RPS6 expression in all cases of OSCC and OED, whereas PD-L1 was expressed in 42/48 (87%) OED and in 28/52 (53%) OSCC. The patients with mild OED presented higher expression level of PD-L1 and p-RPS6 significantly, when compared to moderate-differentiated OSCC patients (p < 0.05). Moreover, we found a significant positive correlation between PD-L1 and p-RPS6 expression in OED and OSCC patients (p < 0.01). The PD-L1 expression was significantly related to more than 2 cm tumor size in OSCC patients (p = 0.007).DiscussionOur findings suggest the upregulation of PD-L1 may be related with activation of the mTOR pathway in the early events of tumor progression and the pathogenesis of OSCC. |
| first_indexed | 2024-03-08T08:25:49Z |
| format | Article |
| id | doaj.art-c49f6c40255a482b88dbf6304feab236 |
| institution | Directory Open Access Journal |
| issn | 2673-4842 |
| language | English |
| last_indexed | 2024-03-08T08:25:49Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oral Health |
| spelling | doaj.art-c49f6c40255a482b88dbf6304feab2362024-02-02T04:48:06ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422024-02-01510.3389/froh.2024.13375821337582Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavityJaruwat Hanroongsri0Panomwat Amornphimoltham1Rania H. Younis2Risa Chaisuparat3Risa Chaisuparat4Division of Oral Diagnostic Sciences, Faculty of Dentistry, Thammasat University, Pathumthani, ThailandDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, ThailandDepartment of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, EgyptDepartment of Oral Pathology, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandAvatar Biotechnologies for Oral Heath and Healthy Longevity, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandIntroductionOral squamous cell carcinoma (OSCC) is often preceded by oral epithelial dysplasia (OED). The role of ribosomal protein S6 (RPS6) and programmed cell death ligand-1 (PD-L1) in the progression of OED to OSCC remains unclear. This study aimed to investigate the expression of phosphorylated RPS6 (p-RPS6) and PD-L1 in OSCC and OED and to examine its relationship with clinicopathological features.MethodsFifty-two OSCC and 48 OED cases were recruited for immunohistochemical analysis of p-RPS6 and PD-L1 expression. The expression of markers was correlated with clinicopathological features of OSCC and OED.ResultsWe found p-RPS6 expression in all cases of OSCC and OED, whereas PD-L1 was expressed in 42/48 (87%) OED and in 28/52 (53%) OSCC. The patients with mild OED presented higher expression level of PD-L1 and p-RPS6 significantly, when compared to moderate-differentiated OSCC patients (p < 0.05). Moreover, we found a significant positive correlation between PD-L1 and p-RPS6 expression in OED and OSCC patients (p < 0.01). The PD-L1 expression was significantly related to more than 2 cm tumor size in OSCC patients (p = 0.007).DiscussionOur findings suggest the upregulation of PD-L1 may be related with activation of the mTOR pathway in the early events of tumor progression and the pathogenesis of OSCC.https://www.frontiersin.org/articles/10.3389/froh.2024.1337582/fulloral epithelial dysplasiaoral squamous cell carcinomaPD-L1RPS6malignant transformation |
| spellingShingle | Jaruwat Hanroongsri Panomwat Amornphimoltham Rania H. Younis Risa Chaisuparat Risa Chaisuparat Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity Frontiers in Oral Health oral epithelial dysplasia oral squamous cell carcinoma PD-L1 RPS6 malignant transformation |
| title | Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| title_full | Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| title_fullStr | Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| title_full_unstemmed | Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| title_short | Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| title_sort | expression of pd l1 and p rps6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity |
| topic | oral epithelial dysplasia oral squamous cell carcinoma PD-L1 RPS6 malignant transformation |
| url | https://www.frontiersin.org/articles/10.3389/froh.2024.1337582/full |
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