Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method
Abstract Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube that does not require a CO2 incubator. In the pres...
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Nature Portfolio
2023-11-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-47017-8 |
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author | Yasuyuki Kikuchi Daiyu Ito Sayaka Wakayama Masatoshi Ooga Teruhiko Wakayama |
author_facet | Yasuyuki Kikuchi Daiyu Ito Sayaka Wakayama Masatoshi Ooga Teruhiko Wakayama |
author_sort | Yasuyuki Kikuchi |
collection | DOAJ |
description | Abstract Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube that does not require a CO2 incubator. In the present study, we developed a new time-lapse observation system using our previous culture method and a glass capillary. Zygotes were placed in a glass capillary and sunk in oil for observation under a stereomicroscope. Warming the capillary using a thermoplate enabled most of the zygotes to develop into blastocysts and produce healthy offspring. This time-lapse observation system captured images every 30 min for up to 5 days, which confirmed that the developmental speed and quality of the embryos were not affected, even with fluorescence. Overall, this new system is a simple time-lapse observation method for preimplantation embryos that does not require dedicated machines and advanced techniques. |
first_indexed | 2024-03-10T17:52:12Z |
format | Article |
id | doaj.art-c4a958b296f949a7815fb97e7624666a |
institution | Directory Open Access Journal |
issn | 2045-2322 |
language | English |
last_indexed | 2024-03-10T17:52:12Z |
publishDate | 2023-11-01 |
publisher | Nature Portfolio |
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series | Scientific Reports |
spelling | doaj.art-c4a958b296f949a7815fb97e7624666a2023-11-20T09:20:14ZengNature PortfolioScientific Reports2045-23222023-11-0113111010.1038/s41598-023-47017-8Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary methodYasuyuki Kikuchi0Daiyu Ito1Sayaka Wakayama2Masatoshi Ooga3Teruhiko Wakayama4Faculty of Life and Environmental Science, University of YamanashiFaculty of Life and Environmental Science, University of YamanashiAdvanced Biotechnology Center, University of YamanashiFaculty of Life and Environmental Science, University of YamanashiAdvanced Biotechnology Center, University of YamanashiAbstract Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube that does not require a CO2 incubator. In the present study, we developed a new time-lapse observation system using our previous culture method and a glass capillary. Zygotes were placed in a glass capillary and sunk in oil for observation under a stereomicroscope. Warming the capillary using a thermoplate enabled most of the zygotes to develop into blastocysts and produce healthy offspring. This time-lapse observation system captured images every 30 min for up to 5 days, which confirmed that the developmental speed and quality of the embryos were not affected, even with fluorescence. Overall, this new system is a simple time-lapse observation method for preimplantation embryos that does not require dedicated machines and advanced techniques.https://doi.org/10.1038/s41598-023-47017-8 |
spellingShingle | Yasuyuki Kikuchi Daiyu Ito Sayaka Wakayama Masatoshi Ooga Teruhiko Wakayama Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method Scientific Reports |
title | Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
title_full | Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
title_fullStr | Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
title_full_unstemmed | Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
title_short | Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
title_sort | time lapse observation of mouse preimplantation embryos using a simple closed glass capillary method |
url | https://doi.org/10.1038/s41598-023-47017-8 |
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