Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots
The production of volatile organic compounds (VOCs) represents a promising strategy of plant-beneficial bacteria to control soil-borne phytopathogens. <i>Pseudomonas</i> sp. PICF6 and <i>Pseudomonas simiae</i> PICF7 are two indigenous inhabitants of olive roots displaying eff...
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/318 |
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| author | Nuria Montes-Osuna Tomislav Cernava Carmen Gómez-Lama Cabanás Gabriele Berg Jesús Mercado-Blanco |
| author_facet | Nuria Montes-Osuna Tomislav Cernava Carmen Gómez-Lama Cabanás Gabriele Berg Jesús Mercado-Blanco |
| author_sort | Nuria Montes-Osuna |
| collection | DOAJ |
| description | The production of volatile organic compounds (VOCs) represents a promising strategy of plant-beneficial bacteria to control soil-borne phytopathogens. <i>Pseudomonas</i> sp. PICF6 and <i>Pseudomonas simiae</i> PICF7 are two indigenous inhabitants of olive roots displaying effective biological control against <i>Verticillium dahliae.</i> Additionally, strain PICF7 is able to promote the growth of barley and <i>Arabidopsis thaliana</i>, VOCs being involved in the growth of the latter species. In this study, the antagonistic capacity of these endophytic bacteria against relevant phytopathogens (<i>Verticillium</i> spp., <i>Rhizoctonia solani</i>, <i>Sclerotinia sclerotiorum</i> and <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i>) was assessed. Under in vitro conditions, PICF6 and PICF7 were only able to antagonize representative isolates of <i>V. dahliae</i> and <i>V. longisporum</i>. Remarkably, both strains produced an impressive portfolio of up to twenty VOCs, that included compounds with reported antifungal (e.g., 1-undecene, (methyldisulfanyl) methane and 1-decene) or plant growth promoting (e.g., tridecane, 1-decene) activities. Moreover, their volatilomes differed strongly in the absence and presence of <i>V. dahliae</i>. For example, when co incubated with the defoliating pathotype of <i>V. dahliae,</i> the antifungal compound 4-methyl-2,6-bis(2-methyl-2-propanyl)phenol was produced. Results suggest that volatiles emitted by these endophytes may differ in their modes of action, and that potential benefits for the host needs further investigation <i>in planta</i>. |
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| language | English |
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| publishDate | 2022-01-01 |
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| spelling | doaj.art-c4ac5b8ba8054b3faa520d0348287cdf2023-11-23T17:29:59ZengMDPI AGPlants2223-77472022-01-0111331810.3390/plants11030318Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive RootsNuria Montes-Osuna0Tomislav Cernava1Carmen Gómez-Lama Cabanás2Gabriele Berg3Jesús Mercado-Blanco4Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avenida Menéndez Pidal s/n, Campus “Alameda del Obispo”, 14004 Cordoba, SpainInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, AustriaDepartamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avenida Menéndez Pidal s/n, Campus “Alameda del Obispo”, 14004 Cordoba, SpainInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, AustriaDepartamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avenida Menéndez Pidal s/n, Campus “Alameda del Obispo”, 14004 Cordoba, SpainThe production of volatile organic compounds (VOCs) represents a promising strategy of plant-beneficial bacteria to control soil-borne phytopathogens. <i>Pseudomonas</i> sp. PICF6 and <i>Pseudomonas simiae</i> PICF7 are two indigenous inhabitants of olive roots displaying effective biological control against <i>Verticillium dahliae.</i> Additionally, strain PICF7 is able to promote the growth of barley and <i>Arabidopsis thaliana</i>, VOCs being involved in the growth of the latter species. In this study, the antagonistic capacity of these endophytic bacteria against relevant phytopathogens (<i>Verticillium</i> spp., <i>Rhizoctonia solani</i>, <i>Sclerotinia sclerotiorum</i> and <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i>) was assessed. Under in vitro conditions, PICF6 and PICF7 were only able to antagonize representative isolates of <i>V. dahliae</i> and <i>V. longisporum</i>. Remarkably, both strains produced an impressive portfolio of up to twenty VOCs, that included compounds with reported antifungal (e.g., 1-undecene, (methyldisulfanyl) methane and 1-decene) or plant growth promoting (e.g., tridecane, 1-decene) activities. Moreover, their volatilomes differed strongly in the absence and presence of <i>V. dahliae</i>. For example, when co incubated with the defoliating pathotype of <i>V. dahliae,</i> the antifungal compound 4-methyl-2,6-bis(2-methyl-2-propanyl)phenol was produced. Results suggest that volatiles emitted by these endophytes may differ in their modes of action, and that potential benefits for the host needs further investigation <i>in planta</i>.https://www.mdpi.com/2223-7747/11/3/318biological control agentsolive rhizobacteria<i>Pseudomonas</i> sp. PICF6<i>Pseudomonas simiae</i> PICF7root endophytes<i>Verticillium dahliae</i> |
| spellingShingle | Nuria Montes-Osuna Tomislav Cernava Carmen Gómez-Lama Cabanás Gabriele Berg Jesús Mercado-Blanco Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots Plants biological control agents olive rhizobacteria <i>Pseudomonas</i> sp. PICF6 <i>Pseudomonas simiae</i> PICF7 root endophytes <i>Verticillium dahliae</i> |
| title | Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots |
| title_full | Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots |
| title_fullStr | Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots |
| title_full_unstemmed | Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots |
| title_short | Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic <i>Pseudomonas</i> Strains from Olive Roots |
| title_sort | identification of volatile organic compounds emitted by two beneficial endophytic i pseudomonas i strains from olive roots |
| topic | biological control agents olive rhizobacteria <i>Pseudomonas</i> sp. PICF6 <i>Pseudomonas simiae</i> PICF7 root endophytes <i>Verticillium dahliae</i> |
| url | https://www.mdpi.com/2223-7747/11/3/318 |
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