Gut and lung microbiome profiles in pregnant mice
In recent years, microbiome research has expanded from the gastrointestinal tract to other host sites previously thought to be abacterial such as the lungs. Yet, the effects of pregnancy in the lung and gut microbiome remains unclear. Here we examined the changes in the gut and lung microbiome in mi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.946779/full |
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| author | Rosana Wiscovitch-Russo Aji Mary Taal Claire Kuelbs Lauren M. Oldfield MohanKumar Ramar Harinder Singh Alexey V. Fedulov Norberto Gonzalez-Juarbe |
| author_facet | Rosana Wiscovitch-Russo Aji Mary Taal Claire Kuelbs Lauren M. Oldfield MohanKumar Ramar Harinder Singh Alexey V. Fedulov Norberto Gonzalez-Juarbe |
| author_sort | Rosana Wiscovitch-Russo |
| collection | DOAJ |
| description | In recent years, microbiome research has expanded from the gastrointestinal tract to other host sites previously thought to be abacterial such as the lungs. Yet, the effects of pregnancy in the lung and gut microbiome remains unclear. Here we examined the changes in the gut and lung microbiome in mice at 14 days of gestation. Lung tissue and stool samples were collected from pregnant and non-pregnant female BALB/c mice, DNA was isolated, amplified, and bacterial specific V4 16S rRNA gene was sequenced. Using an in-house bioinformatic pipeline we assessed the microbial composition of each organ using stool and lung tissue samples. The stool data showed that Lachnospiraceae and Lactobacillaceae were more abundant in the pregnant mice. Likewise, Lactobacillaceae were dominant in the lungs of pregnant mice. However, Streptococcaceae were dominant in the lungs of non-pregnant mice with a low microbial abundance in the pregnant mice. A permutation test showed that pregnancy significantly contributes to the variance in both the lung and stool microbiome. At the same time, we estimate that 49% of the total detected operational taxonomic units were shared between the stool and lung data. After removing common stool-associated bacteria from the lung dataset, no microbial differential abundance was detected between the pregnant and non-pregnant lung microbial community. Thus, pregnancy contributes to variance to the lung and stool microbiome but not in the unique lung microbiota. |
| first_indexed | 2024-04-12T02:45:35Z |
| format | Article |
| id | doaj.art-c4b003b2acef4209ba39851ad69c262a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T02:45:35Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c4b003b2acef4209ba39851ad69c262a2022-12-22T03:51:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.946779946779Gut and lung microbiome profiles in pregnant miceRosana Wiscovitch-Russo0Aji Mary Taal1Claire Kuelbs2Lauren M. Oldfield3MohanKumar Ramar4Harinder Singh5Alexey V. Fedulov6Norberto Gonzalez-Juarbe7J. Craig Venter Institute, Rockville, MD, United StatesJ. Craig Venter Institute, Rockville, MD, United StatesJ. Craig Venter Institute, Rockville, MD, United StatesJ. Craig Venter Institute, Rockville, MD, United StatesDepartment of Surgery, Division of Surgical Research, Rhode Island Hospital, The Warren Alpert Medical School of Brown University, Providence, RI, United StatesJ. Craig Venter Institute, Rockville, MD, United StatesDepartment of Surgery, Division of Surgical Research, Rhode Island Hospital, The Warren Alpert Medical School of Brown University, Providence, RI, United StatesJ. Craig Venter Institute, Rockville, MD, United StatesIn recent years, microbiome research has expanded from the gastrointestinal tract to other host sites previously thought to be abacterial such as the lungs. Yet, the effects of pregnancy in the lung and gut microbiome remains unclear. Here we examined the changes in the gut and lung microbiome in mice at 14 days of gestation. Lung tissue and stool samples were collected from pregnant and non-pregnant female BALB/c mice, DNA was isolated, amplified, and bacterial specific V4 16S rRNA gene was sequenced. Using an in-house bioinformatic pipeline we assessed the microbial composition of each organ using stool and lung tissue samples. The stool data showed that Lachnospiraceae and Lactobacillaceae were more abundant in the pregnant mice. Likewise, Lactobacillaceae were dominant in the lungs of pregnant mice. However, Streptococcaceae were dominant in the lungs of non-pregnant mice with a low microbial abundance in the pregnant mice. A permutation test showed that pregnancy significantly contributes to the variance in both the lung and stool microbiome. At the same time, we estimate that 49% of the total detected operational taxonomic units were shared between the stool and lung data. After removing common stool-associated bacteria from the lung dataset, no microbial differential abundance was detected between the pregnant and non-pregnant lung microbial community. Thus, pregnancy contributes to variance to the lung and stool microbiome but not in the unique lung microbiota.https://www.frontiersin.org/articles/10.3389/fmicb.2022.946779/fullmicrobiomepregnancystoollung16S sequence |
| spellingShingle | Rosana Wiscovitch-Russo Aji Mary Taal Claire Kuelbs Lauren M. Oldfield MohanKumar Ramar Harinder Singh Alexey V. Fedulov Norberto Gonzalez-Juarbe Gut and lung microbiome profiles in pregnant mice Frontiers in Microbiology microbiome pregnancy stool lung 16S sequence |
| title | Gut and lung microbiome profiles in pregnant mice |
| title_full | Gut and lung microbiome profiles in pregnant mice |
| title_fullStr | Gut and lung microbiome profiles in pregnant mice |
| title_full_unstemmed | Gut and lung microbiome profiles in pregnant mice |
| title_short | Gut and lung microbiome profiles in pregnant mice |
| title_sort | gut and lung microbiome profiles in pregnant mice |
| topic | microbiome pregnancy stool lung 16S sequence |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.946779/full |
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