Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm
Aluminum (Al) toxicity is a major abiotic stress that negatively impacts plant growth and crop productivity. Al ions are released into soil solutions as a function of soil pH, which is in turn determined by a combination of factors, including local geology, historic vegetation and land-use patterns....
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/4/948 |
| _version_ | 1797437257706110976 |
|---|---|
| author | Hawi Negusse Douglas R. Cook Teklehaimanot Haileselassie Kassahun Tesfaye |
| author_facet | Hawi Negusse Douglas R. Cook Teklehaimanot Haileselassie Kassahun Tesfaye |
| author_sort | Hawi Negusse |
| collection | DOAJ |
| description | Aluminum (Al) toxicity is a major abiotic stress that negatively impacts plant growth and crop productivity. Al ions are released into soil solutions as a function of soil pH, which is in turn determined by a combination of factors, including local geology, historic vegetation and land-use patterns. Selection and use of Al-tolerant crops is a preferred method to address the problem of Al toxicity. The present study evaluated a combination of modern cultivars, advanced breeding lines and a local landrace for Al tolerance using a seedling-based hydroponic assay. Two sequential experiments were conducted to score root and shoot traits in the presence of aluminum. Initially, six Al concentrations (0, 50, 100, 120, 150 and 200 µM) were tested on six chickpea genotypes to identify the single Al concentration that best discriminates among genotypes. Subsequently, 31 chickpea genotypes were evaluated at 0 and 120 µM Al. Progressive declines in trait values were observed in all genotypes with increasing Al, although the degree of sensitivity varied significantly among genotypes. Genotypes were evaluated both for total root length under 120 µM Al and for relative root growth compared to a 0 µM Al control treatment. Considering both parameters, we identified four tolerant chickpea genotypes (<i>DZ-2012-CK-0237</i>, <i>Wollega LV</i>, <i>DZ-2012-CK-0233</i> and <i>Natoli</i>) and two sensitive genotypes (<i>Akaki</i> and <i>Fetenech</i>). <i>Wollega LV</i> is a local landrace obtained from acidic soil regions of Western Ethiopia, presenting the possibility that historical selection during cultivation on acidic soils might underlie its unusual tolerance. The aluminum tolerance traits identified here are candidates for introgression breeding of new Ethiopian chickpea varieties with potential to increase yield and expand the area of cultivation. |
| first_indexed | 2024-03-09T11:17:38Z |
| format | Article |
| id | doaj.art-c4b13457bf97464392494d8a5b33d89d |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T11:17:38Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c4b13457bf97464392494d8a5b33d89d2023-12-01T00:29:17ZengMDPI AGAgronomy2073-43952022-04-0112494810.3390/agronomy12040948Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) GermplasmHawi Negusse0Douglas R. Cook1Teklehaimanot Haileselassie2Kassahun Tesfaye3Institute of Biotechnology, Addis Ababa University, Addis Ababa 1000, EthiopiaDepartment of Plant Pathology, University of California, Davis, CA 95616, USAInstitute of Biotechnology, Addis Ababa University, Addis Ababa 1000, EthiopiaInstitute of Biotechnology, Addis Ababa University, Addis Ababa 1000, EthiopiaAluminum (Al) toxicity is a major abiotic stress that negatively impacts plant growth and crop productivity. Al ions are released into soil solutions as a function of soil pH, which is in turn determined by a combination of factors, including local geology, historic vegetation and land-use patterns. Selection and use of Al-tolerant crops is a preferred method to address the problem of Al toxicity. The present study evaluated a combination of modern cultivars, advanced breeding lines and a local landrace for Al tolerance using a seedling-based hydroponic assay. Two sequential experiments were conducted to score root and shoot traits in the presence of aluminum. Initially, six Al concentrations (0, 50, 100, 120, 150 and 200 µM) were tested on six chickpea genotypes to identify the single Al concentration that best discriminates among genotypes. Subsequently, 31 chickpea genotypes were evaluated at 0 and 120 µM Al. Progressive declines in trait values were observed in all genotypes with increasing Al, although the degree of sensitivity varied significantly among genotypes. Genotypes were evaluated both for total root length under 120 µM Al and for relative root growth compared to a 0 µM Al control treatment. Considering both parameters, we identified four tolerant chickpea genotypes (<i>DZ-2012-CK-0237</i>, <i>Wollega LV</i>, <i>DZ-2012-CK-0233</i> and <i>Natoli</i>) and two sensitive genotypes (<i>Akaki</i> and <i>Fetenech</i>). <i>Wollega LV</i> is a local landrace obtained from acidic soil regions of Western Ethiopia, presenting the possibility that historical selection during cultivation on acidic soils might underlie its unusual tolerance. The aluminum tolerance traits identified here are candidates for introgression breeding of new Ethiopian chickpea varieties with potential to increase yield and expand the area of cultivation.https://www.mdpi.com/2073-4395/12/4/948aluminum tolerancealuminum toxicity<i>Cicer arietinum</i>modern cultivarssoil acidity |
| spellingShingle | Hawi Negusse Douglas R. Cook Teklehaimanot Haileselassie Kassahun Tesfaye Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm Agronomy aluminum tolerance aluminum toxicity <i>Cicer arietinum</i> modern cultivars soil acidity |
| title | Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm |
| title_full | Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm |
| title_fullStr | Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm |
| title_full_unstemmed | Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm |
| title_short | Identification of Aluminum Tolerance in Ethiopian Chickpea (<i>Cicer arietinum</i> L.) Germplasm |
| title_sort | identification of aluminum tolerance in ethiopian chickpea i cicer arietinum i l germplasm |
| topic | aluminum tolerance aluminum toxicity <i>Cicer arietinum</i> modern cultivars soil acidity |
| url | https://www.mdpi.com/2073-4395/12/4/948 |
| work_keys_str_mv | AT hawinegusse identificationofaluminumtoleranceinethiopianchickpeaicicerarietinumilgermplasm AT douglasrcook identificationofaluminumtoleranceinethiopianchickpeaicicerarietinumilgermplasm AT teklehaimanothaileselassie identificationofaluminumtoleranceinethiopianchickpeaicicerarietinumilgermplasm AT kassahuntesfaye identificationofaluminumtoleranceinethiopianchickpeaicicerarietinumilgermplasm |