Establishment of a blunt impact-induced brain injury model in rabbits
【Abstract】 Objective: To establish an animal model to replicate the blunt impact brain injury in forensic medicine. Methods: Twenty-four New Zealand white rabbits were randomly divided into control group (n=4), minor injury group (n=10) and severe injury group (n=10). Based on the BIM-Ⅱ H...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-04-01
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| Series: | Chinese Journal of Traumatology |
| Online Access: | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/208 |
| _version_ | 1819207477372125184 |
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| author | LI Kui CAO Yun-xing YANG Yong-qiang YIN Zhi-yong ZHAO Hui WANG Li-jun |
| author_facet | LI Kui CAO Yun-xing YANG Yong-qiang YIN Zhi-yong ZHAO Hui WANG Li-jun |
| author_sort | LI Kui |
| collection | DOAJ |
| description | 【Abstract】 Objective: To establish an animal model
to replicate the blunt impact brain injury in forensic medicine.
Methods: Twenty-four New Zealand white rabbits were
randomly divided into control group (n=4), minor injury
group (n=10) and severe injury group (n=10). Based on the
BIM-Ⅱ Horizontal Bio-impact Machine, self-designed iron
bar was used to produce blunt brain injury. Two rabbits
from each injury group were randomly selected to monitor
the change of intracranial pressure (ICP) during the impact-ing process by pressure microsensors. Six hours after injury,
all the rabbits were dissected to observe the injury mor-phology and underwent routine pathological examination.
Results: Varying degrees of nervous system positive
signs were observed in all the injured rabbits. Within 6 hours,
the mortality rate was 1/10 in the minor injury group and 6/10
in the severe injury group. Morphological changes con-sisted of different levels of scalp hematoma, skull fracture,
epidural hematoma, subdural hematoma, subarachnoid hemo-rrhage and brain injury. At the moment of hitting, the ICP
was greater in severe injury group than in mild injury group;
and within the same group, the impact side showed positive
pressure while the opposite side showed negative pressure.
Conclusions: Under the rigidly-controlled experimen-tal condition, this animal model has a good reproducibility
and stable results. Meanwhile, it is able to simulate the mor-phology of iron strike-induced injury, thus can be used to
study the mechanism of blunt head injury in forensic
medicine.
Key words: Brain injuries; Forensic medicine;
Wounds, nonpenetrating; Models, animal; Rabbits |
| first_indexed | 2024-12-23T05:24:07Z |
| format | Article |
| id | doaj.art-c4b197b5019f44938ffc95e6f6cb0847 |
| institution | Directory Open Access Journal |
| issn | 1008-1275 |
| language | English |
| last_indexed | 2024-12-23T05:24:07Z |
| publishDate | 2012-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chinese Journal of Traumatology |
| spelling | doaj.art-c4b197b5019f44938ffc95e6f6cb08472022-12-21T17:58:38ZengElsevierChinese Journal of Traumatology1008-12752012-04-01152100104129Establishment of a blunt impact-induced brain injury model in rabbitsLI KuiCAO Yun-xingYANG Yong-qiangYIN Zhi-yongZHAO HuiWANG Li-jun【Abstract】 Objective: To establish an animal model to replicate the blunt impact brain injury in forensic medicine. Methods: Twenty-four New Zealand white rabbits were randomly divided into control group (n=4), minor injury group (n=10) and severe injury group (n=10). Based on the BIM-Ⅱ Horizontal Bio-impact Machine, self-designed iron bar was used to produce blunt brain injury. Two rabbits from each injury group were randomly selected to monitor the change of intracranial pressure (ICP) during the impact-ing process by pressure microsensors. Six hours after injury, all the rabbits were dissected to observe the injury mor-phology and underwent routine pathological examination. Results: Varying degrees of nervous system positive signs were observed in all the injured rabbits. Within 6 hours, the mortality rate was 1/10 in the minor injury group and 6/10 in the severe injury group. Morphological changes con-sisted of different levels of scalp hematoma, skull fracture, epidural hematoma, subdural hematoma, subarachnoid hemo-rrhage and brain injury. At the moment of hitting, the ICP was greater in severe injury group than in mild injury group; and within the same group, the impact side showed positive pressure while the opposite side showed negative pressure. Conclusions: Under the rigidly-controlled experimen-tal condition, this animal model has a good reproducibility and stable results. Meanwhile, it is able to simulate the mor-phology of iron strike-induced injury, thus can be used to study the mechanism of blunt head injury in forensic medicine. Key words: Brain injuries; Forensic medicine; Wounds, nonpenetrating; Models, animal; Rabbitshttp://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/208 |
| spellingShingle | LI Kui CAO Yun-xing YANG Yong-qiang YIN Zhi-yong ZHAO Hui WANG Li-jun Establishment of a blunt impact-induced brain injury model in rabbits Chinese Journal of Traumatology |
| title | Establishment of a blunt impact-induced brain injury model in rabbits |
| title_full | Establishment of a blunt impact-induced brain injury model in rabbits |
| title_fullStr | Establishment of a blunt impact-induced brain injury model in rabbits |
| title_full_unstemmed | Establishment of a blunt impact-induced brain injury model in rabbits |
| title_short | Establishment of a blunt impact-induced brain injury model in rabbits |
| title_sort | establishment of a blunt impact induced brain injury model in rabbits |
| url | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/208 |
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