INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS
Chalcones are open chain molecules precursors of flavonoids and isoflavonoids, found spread in edible plants. Because they are easily accessible trough Claisen Shmidt condensation, a great variety of derivatives are available. They have also shown potential in pharmacological and biological applicat...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
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| Series: | Química Nova |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000800914&lng=en&tlng=en |
| _version_ | 1819138589485694976 |
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| author | Ximena Zarate Eduardo Schott Carlos A. Escobar Roberto Lopez-Castro Cesar Echeverria Leonor Alvarado-Soto Rodrigo Ramirez-Tagle |
| author_facet | Ximena Zarate Eduardo Schott Carlos A. Escobar Roberto Lopez-Castro Cesar Echeverria Leonor Alvarado-Soto Rodrigo Ramirez-Tagle |
| author_sort | Ximena Zarate |
| collection | DOAJ |
| description | Chalcones are open chain molecules precursors of flavonoids and isoflavonoids, found spread in edible plants. Because they are easily accessible trough Claisen Shmidt condensation, a great variety of derivatives are available. They have also shown potential in pharmacological and biological applications. It is known that chalcone derivatives display a role in the treatment of complex diseases such as cancer, among others, where the DNA is considered as the target for the action of these kinds of compounds. This action is commonly explained as the inhibition of the DNA replications and transcriptions through interactions. However, not conclusive associations between these DNA-Drug interactions and toxicity have been found. This research focuses on the capacity of a chalcone`s family to interact with DNA. Therefore, the binding constants for each compounds with Calf Thymus DNA [CT-DNA] were determined by spectrophotometric titration at room temperature. In addition, the effect of increasing the chalcone`s concentration over the relative viscosity of CT-DNA at room temperature was assessed. On the other hand, with the aim to find the optimal DNA-chalcone configurations, as well as consistently predict their binding, a computational work was undertaken. To accomplish these goals within a reasonable time framework, an empirical scoring function (AScore) and a docking engine (ShapeDock) were performed using the ArgusLab package. The results of viscosity and docking measurement provided structural insights which suggest that chalcones bind with DNA via interaction as well as intercalation. The presence of interactions is also evidenced by the spectrophotometric study which showed luminescence quenching of the chalcones upon interaction with CT-DNA. |
| first_indexed | 2024-12-22T11:09:10Z |
| format | Article |
| id | doaj.art-c4b43f36b36145cbb7e9eca560f94ab0 |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-12-22T11:09:10Z |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-c4b43f36b36145cbb7e9eca560f94ab02022-12-21T18:28:13ZengSociedade Brasileira de QuímicaQuímica Nova1678-706439891491810.5935/0100-4042.20160114S0100-40422016000800914INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONSXimena ZarateEduardo SchottCarlos A. EscobarRoberto Lopez-CastroCesar EcheverriaLeonor Alvarado-SotoRodrigo Ramirez-TagleChalcones are open chain molecules precursors of flavonoids and isoflavonoids, found spread in edible plants. Because they are easily accessible trough Claisen Shmidt condensation, a great variety of derivatives are available. They have also shown potential in pharmacological and biological applications. It is known that chalcone derivatives display a role in the treatment of complex diseases such as cancer, among others, where the DNA is considered as the target for the action of these kinds of compounds. This action is commonly explained as the inhibition of the DNA replications and transcriptions through interactions. However, not conclusive associations between these DNA-Drug interactions and toxicity have been found. This research focuses on the capacity of a chalcone`s family to interact with DNA. Therefore, the binding constants for each compounds with Calf Thymus DNA [CT-DNA] were determined by spectrophotometric titration at room temperature. In addition, the effect of increasing the chalcone`s concentration over the relative viscosity of CT-DNA at room temperature was assessed. On the other hand, with the aim to find the optimal DNA-chalcone configurations, as well as consistently predict their binding, a computational work was undertaken. To accomplish these goals within a reasonable time framework, an empirical scoring function (AScore) and a docking engine (ShapeDock) were performed using the ArgusLab package. The results of viscosity and docking measurement provided structural insights which suggest that chalcones bind with DNA via interaction as well as intercalation. The presence of interactions is also evidenced by the spectrophotometric study which showed luminescence quenching of the chalcones upon interaction with CT-DNA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000800914&lng=en&tlng=enchalconesDNAdockingantitumoralspectrophotometric analysis |
| spellingShingle | Ximena Zarate Eduardo Schott Carlos A. Escobar Roberto Lopez-Castro Cesar Echeverria Leonor Alvarado-Soto Rodrigo Ramirez-Tagle INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS Química Nova chalcones DNA docking antitumoral spectrophotometric analysis |
| title | INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS |
| title_full | INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS |
| title_fullStr | INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS |
| title_full_unstemmed | INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS |
| title_short | INTERACTION OF CHALCONES WITH CT-DNA BY SPECTROPHOTOMETRIC ANALYSIS AND THEORETICALSIMULATIONS |
| title_sort | interaction of chalcones with ct dna by spectrophotometric analysis and theoreticalsimulations |
| topic | chalcones DNA docking antitumoral spectrophotometric analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000800914&lng=en&tlng=en |
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