The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension
The purpose of this study was to assess oxidative phosphorylation (OXPHOS) in mouse ovaries, determine the relative content of proteins that form the respiratory chain complexes and the main structures of the cytoskeleton, and determine the mRNA of the corresponding genes after hindlimb suspension f...
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MDPI AG
2023-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/12/2332 |
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| author | Elena Yu. Gorbacheva Maria A. Sventitskaya Nikolay S. Biryukov Irina V. Ogneva |
| author_facet | Elena Yu. Gorbacheva Maria A. Sventitskaya Nikolay S. Biryukov Irina V. Ogneva |
| author_sort | Elena Yu. Gorbacheva |
| collection | DOAJ |
| description | The purpose of this study was to assess oxidative phosphorylation (OXPHOS) in mouse ovaries, determine the relative content of proteins that form the respiratory chain complexes and the main structures of the cytoskeleton, and determine the mRNA of the corresponding genes after hindlimb suspension for 96 h. After hindlimb suspension, the maximum rate of oxygen uptake increased by 133% (<i>p</i> < 0.05) compared to the control due to the complex I of the respiratory chain. The content of mRNA of genes encoding the main components of the respiratory chain increased (cyt <i>c</i> by 78%, cox IV by 56%, ATPase by 69%, <i>p</i> < 0.05 compared with the control). The relative content of cytoskeletal proteins that can participate in the processes of transport and localization of mitochondria does not change, with the exception of an increase in the content of alpha-tubulin by 25% (<i>p</i> < 0.05) and its acetylated isoform (by 36%, <i>p</i> < 0.05); however, the mRNA content of these cytoskeletal genes did not differ from the control. The content of GDF9 mRNA does not change after hindlimb suspension. The data obtained show that short-term exposure to simulated weightlessness leads to intensification of metabolism in the ovaries. |
| first_indexed | 2024-03-08T20:36:11Z |
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| id | doaj.art-c4b484843f5f4687971c53793826fa48 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T20:36:11Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-c4b484843f5f4687971c53793826fa482023-12-22T14:21:21ZengMDPI AGLife2075-17292023-12-011312233210.3390/life13122332The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb SuspensionElena Yu. Gorbacheva0Maria A. Sventitskaya1Nikolay S. Biryukov2Irina V. Ogneva3Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, Moscow 123007, RussiaThe purpose of this study was to assess oxidative phosphorylation (OXPHOS) in mouse ovaries, determine the relative content of proteins that form the respiratory chain complexes and the main structures of the cytoskeleton, and determine the mRNA of the corresponding genes after hindlimb suspension for 96 h. After hindlimb suspension, the maximum rate of oxygen uptake increased by 133% (<i>p</i> < 0.05) compared to the control due to the complex I of the respiratory chain. The content of mRNA of genes encoding the main components of the respiratory chain increased (cyt <i>c</i> by 78%, cox IV by 56%, ATPase by 69%, <i>p</i> < 0.05 compared with the control). The relative content of cytoskeletal proteins that can participate in the processes of transport and localization of mitochondria does not change, with the exception of an increase in the content of alpha-tubulin by 25% (<i>p</i> < 0.05) and its acetylated isoform (by 36%, <i>p</i> < 0.05); however, the mRNA content of these cytoskeletal genes did not differ from the control. The content of GDF9 mRNA does not change after hindlimb suspension. The data obtained show that short-term exposure to simulated weightlessness leads to intensification of metabolism in the ovaries.https://www.mdpi.com/2075-1729/13/12/2332OXPHOSmicrogravityovarycytoskeletongene expression |
| spellingShingle | Elena Yu. Gorbacheva Maria A. Sventitskaya Nikolay S. Biryukov Irina V. Ogneva The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension Life OXPHOS microgravity ovary cytoskeleton gene expression |
| title | The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension |
| title_full | The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension |
| title_fullStr | The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension |
| title_full_unstemmed | The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension |
| title_short | The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension |
| title_sort | oxidative phosphorylation and cytoskeleton proteins of mouse ovaries after 96 hours of hindlimb suspension |
| topic | OXPHOS microgravity ovary cytoskeleton gene expression |
| url | https://www.mdpi.com/2075-1729/13/12/2332 |
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