Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction
Fritillaria imperialis L. (Liliaceae) is a rare and endangered ornamental plant grown in mountain regions and Zagros altitudes, Ilam province, Iran. This species is in danger of extinction due to invasive collection. Plant regeneration was done by organogenesis from bulb scales as explants cultured...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2020-02-01
|
| Series: | Advances in Horticultural Science |
| Subjects: | |
| Online Access: | https://oaj.fupress.net/index.php/ahs/article/view/8128 |
| _version_ | 1828491931412332544 |
|---|---|
| author | Shima Seydi Shahram Sedaghathoor Behzad Kaviani |
| author_facet | Shima Seydi Shahram Sedaghathoor Behzad Kaviani |
| author_sort | Shima Seydi |
| collection | DOAJ |
| description | Fritillaria imperialis L. (Liliaceae) is a rare and endangered ornamental plant grown in mountain regions and Zagros altitudes, Ilam province, Iran. This species is in danger of extinction due to invasive collection. Plant regeneration was done by organogenesis from bulb scales as explants cultured on Murashige and Skoog (MS) media fortified with different concentrations of kinetin (KIN, 0.00, 0.50, 1.00 and 2.00 mg l-1) and α_naphthaleneacetic acid (NAA, 0.00, 0.50, 1.00 and 2.00 mg l-1), either individually or in combination. The largest number of leaf (3.80), root (5.86) and callus (8.16) per explant was regenerated on the medium containing 0.50 mg l-1 KIN and 1.00 mg l-1 NAA. Maximum viability percentage (96.66%) was obtained in medium supplemented with 1.00 mg l-1 KIN. In vitro regenerated plantlets were cultivated in plastic pots containing peat moss and perlite (1:1). The plantlets were successfully acclimatized in an adaptation greenhouse with a survival rate of 95% exhibiting normal developmental patterns. |
| first_indexed | 2024-12-11T11:11:28Z |
| format | Article |
| id | doaj.art-c4b5644678654b659ce0cbb574b48489 |
| institution | Directory Open Access Journal |
| issn | 0394-6169 1592-1573 |
| language | English |
| last_indexed | 2024-12-11T11:11:28Z |
| publishDate | 2020-02-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Advances in Horticultural Science |
| spelling | doaj.art-c4b5644678654b659ce0cbb574b484892022-12-22T01:09:31ZengFirenze University PressAdvances in Horticultural Science0394-61691592-15732020-02-0133410.13128/ahsc-8128Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinctionShima Seydi0Shahram Sedaghathoor1Behzad Kaviani2Department of Horticultural Science, Rahst Branch, Ismalic Azad University, RashtDepartment of Horticultural Science, Rahst Branch, Ismalic Azad University, RashtDepartment of Horticultural Science, Rahst Branch, Ismalic Azad University, RashtFritillaria imperialis L. (Liliaceae) is a rare and endangered ornamental plant grown in mountain regions and Zagros altitudes, Ilam province, Iran. This species is in danger of extinction due to invasive collection. Plant regeneration was done by organogenesis from bulb scales as explants cultured on Murashige and Skoog (MS) media fortified with different concentrations of kinetin (KIN, 0.00, 0.50, 1.00 and 2.00 mg l-1) and α_naphthaleneacetic acid (NAA, 0.00, 0.50, 1.00 and 2.00 mg l-1), either individually or in combination. The largest number of leaf (3.80), root (5.86) and callus (8.16) per explant was regenerated on the medium containing 0.50 mg l-1 KIN and 1.00 mg l-1 NAA. Maximum viability percentage (96.66%) was obtained in medium supplemented with 1.00 mg l-1 KIN. In vitro regenerated plantlets were cultivated in plastic pots containing peat moss and perlite (1:1). The plantlets were successfully acclimatized in an adaptation greenhouse with a survival rate of 95% exhibiting normal developmental patterns.https://oaj.fupress.net/index.php/ahs/article/view/8128crown imperialmicropropagationplant growth regulatorsthreatened ornamentalstissue culture |
| spellingShingle | Shima Seydi Shahram Sedaghathoor Behzad Kaviani Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction Advances in Horticultural Science crown imperial micropropagation plant growth regulators threatened ornamentals tissue culture |
| title | Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction |
| title_full | Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction |
| title_fullStr | Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction |
| title_full_unstemmed | Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction |
| title_short | Plant regeneration by organogenesis from bulbous explants in Fritillaria imperialis L., a wild rare ornamental species at the risk of extinction |
| title_sort | plant regeneration by organogenesis from bulbous explants in fritillaria imperialis l a wild rare ornamental species at the risk of extinction |
| topic | crown imperial micropropagation plant growth regulators threatened ornamentals tissue culture |
| url | https://oaj.fupress.net/index.php/ahs/article/view/8128 |
| work_keys_str_mv | AT shimaseydi plantregenerationbyorganogenesisfrombulbousexplantsinfritillariaimperialislawildrareornamentalspeciesattheriskofextinction AT shahramsedaghathoor plantregenerationbyorganogenesisfrombulbousexplantsinfritillariaimperialislawildrareornamentalspeciesattheriskofextinction AT behzadkaviani plantregenerationbyorganogenesisfrombulbousexplantsinfritillariaimperialislawildrareornamentalspeciesattheriskofextinction |