CRISPR Cas9-mediated ablation of pyruvate carboxylase gene in colon cancer cell line HT-29 inhibits growth and migration, induces apoptosis and increases sensitivity to 5-fluorouracil and glutaminase inhibitor

CRISPR Cas9-mediated ablation of pyruvate carboxylase gene in colon cancer cell line HT-29 inhibits growth and migration, induces apoptosis and increases sensitivity to 5-fluorouracil and glutaminase inhibitor

Pyruvate carboxylase (PC) is an important anaplerotic enzyme that replenishes the tricarboxylic acid cycle (TCA) intermediates. It prevents the collapse of the TCA cycle upon its intermediates are removed during high anabolic demand. We have recently shown that overexpression of PC protein was assoc...

Full description

Bibliographic Details
Main Authors: Jarunya Ngamkham, Siraprapa Siritutsoontorn, Saowaluk Saisomboon, Kulthida Vaeteewoottacharn, Sarawut Jitrapakdee
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Oncology
Subjects:
pyruvate carboxylase
cancer metabolism
colorectal cancer
5-FU
glutaminase
Online Access:https://www.frontiersin.org/articles/10.3389/fonc.2022.966089/full

Internet

https://www.frontiersin.org/articles/10.3389/fonc.2022.966089/full

Similar Items