Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia
Abstract Background Ethiopia lies in the high-risk corridor of esophageal squamous cell carcinoma in East Africa, where individuals with this malignancy often do not report established risk factors, suggesting unidentified etiologies. Here, we report the prevalence of mucosal human papillomavirus (H...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Infectious Agents and Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13027-019-0233-x |
| _version_ | 1828860952219484160 |
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| author | Maria E. Leon Endale Kassa Abate Bane Tufa Gemechu Yared Tilahun Nigatu Endalafer Sandrine McKay-Chopin Rosario N. Brancaccio Gilles Ferro Mathewos Assefa Elizabeth Ward Massimo Tommasino Abraham Aseffa Joachim Schüz Ahmedin Jemal Tarik Gheit |
| author_facet | Maria E. Leon Endale Kassa Abate Bane Tufa Gemechu Yared Tilahun Nigatu Endalafer Sandrine McKay-Chopin Rosario N. Brancaccio Gilles Ferro Mathewos Assefa Elizabeth Ward Massimo Tommasino Abraham Aseffa Joachim Schüz Ahmedin Jemal Tarik Gheit |
| author_sort | Maria E. Leon |
| collection | DOAJ |
| description | Abstract Background Ethiopia lies in the high-risk corridor of esophageal squamous cell carcinoma in East Africa, where individuals with this malignancy often do not report established risk factors, suggesting unidentified etiologies. Here, we report the prevalence of mucosal human papillomavirus (HPV) and of Helicobacter pylori (H. pylori) detection in endoscopy-obtained esophageal and gastroesophageal junction biopsies and in oral cell specimens taken at the time of esophageal cancer diagnosis in a case–control study in Addis Ababa, Ethiopia. Methods DNA extraction was performed from fresh frozen tissue and oral cell pellets obtained with saline solution gargling subsequently fixed with ethanol. Mucosal HPV and H. pylori DNA was detected using highly sensitive assays that combine multiplex polymerase chain reaction and bead-based Luminex technology. The proportions of specimens testing positive were expressed as percentages, with binomial 95% confidence intervals. Agreement of results between tissue biopsy and oral cell specimens was estimated using the kappa statistic. Comparison of study participants’ characteristics by test results was done using the Pearson chi-square test. Results HPV DNA was detected in 1 of 62 tumor specimens (2, 95% confidence interval (CI): 0–9%), corresponding to HPV16 type. HPV DNA was detected in the oral cavity of 7 cases (11, 95% CI: 5–22%) and 4 of 56 matched healthy controls (7, 95% CI: 2–17%), with multiple HPV types detected. Detection of H. pylori DNA was 55% (95% CI: 42–68%), and 20 of 34 H. pylori-positive specimens (59, 95% CI: 41–75%) were positive for the cagA gene. Agreement of detection rates between tissue and oral cells in cases was poor for HPV and for H. pylori. Conclusions The prevalence of mucosal-type HPV was very low, whereas H. pylori was more commonly detected, with a high proportion testing positive for the pro-inflammatory gene cagA. These novel findings remain to be replicated in larger studies and with the addition of serological determinations to better understand their biological significance in the context of esophageal and gastroesophageal junction cancers. |
| first_indexed | 2024-12-13T02:50:34Z |
| format | Article |
| id | doaj.art-c4b9b101c9db4c2f90e8a6aa1a3b13a4 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-13T02:50:34Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-c4b9b101c9db4c2f90e8a6aa1a3b13a42022-12-22T00:02:04ZengBMCInfectious Agents and Cancer1750-93782019-08-0114111010.1186/s13027-019-0233-xPrevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in EthiopiaMaria E. Leon0Endale Kassa1Abate Bane2Tufa Gemechu3Yared Tilahun4Nigatu Endalafer5Sandrine McKay-Chopin6Rosario N. Brancaccio7Gilles Ferro8Mathewos Assefa9Elizabeth Ward10Massimo Tommasino11Abraham Aseffa12Joachim Schüz13Ahmedin Jemal14Tarik Gheit15Section of Environment and Radiation, International Agency for Research on Cancer (IARC)Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Addis Ababa UniversityGastroenterology, Department of Internal Medicine, Faculty of Medicine, Addis Ababa UniversityPathology, Faculty of Medicine, Addis Ababa UniversityDepartment of Internal Medicine, Faculty of Medicine, Addis Ababa UniversityArmauer Hansen Research Institute (AHRI)Section of Infections, International Agency for Research on Cancer (IARC)Section of Infections, International Agency for Research on Cancer (IARC)Section of Environment and Radiation, International Agency for Research on Cancer (IARC)Department of Internal Medicine, Faculty of Medicine, Addis Ababa UniversitySurveillance and Health Services Research, American Cancer Society (ACS)Section of Infections, International Agency for Research on Cancer (IARC)Armauer Hansen Research Institute (AHRI)Section of Environment and Radiation, International Agency for Research on Cancer (IARC)Surveillance and Health Services Research, American Cancer Society (ACS)Section of Infections, International Agency for Research on Cancer (IARC)Abstract Background Ethiopia lies in the high-risk corridor of esophageal squamous cell carcinoma in East Africa, where individuals with this malignancy often do not report established risk factors, suggesting unidentified etiologies. Here, we report the prevalence of mucosal human papillomavirus (HPV) and of Helicobacter pylori (H. pylori) detection in endoscopy-obtained esophageal and gastroesophageal junction biopsies and in oral cell specimens taken at the time of esophageal cancer diagnosis in a case–control study in Addis Ababa, Ethiopia. Methods DNA extraction was performed from fresh frozen tissue and oral cell pellets obtained with saline solution gargling subsequently fixed with ethanol. Mucosal HPV and H. pylori DNA was detected using highly sensitive assays that combine multiplex polymerase chain reaction and bead-based Luminex technology. The proportions of specimens testing positive were expressed as percentages, with binomial 95% confidence intervals. Agreement of results between tissue biopsy and oral cell specimens was estimated using the kappa statistic. Comparison of study participants’ characteristics by test results was done using the Pearson chi-square test. Results HPV DNA was detected in 1 of 62 tumor specimens (2, 95% confidence interval (CI): 0–9%), corresponding to HPV16 type. HPV DNA was detected in the oral cavity of 7 cases (11, 95% CI: 5–22%) and 4 of 56 matched healthy controls (7, 95% CI: 2–17%), with multiple HPV types detected. Detection of H. pylori DNA was 55% (95% CI: 42–68%), and 20 of 34 H. pylori-positive specimens (59, 95% CI: 41–75%) were positive for the cagA gene. Agreement of detection rates between tissue and oral cells in cases was poor for HPV and for H. pylori. Conclusions The prevalence of mucosal-type HPV was very low, whereas H. pylori was more commonly detected, with a high proportion testing positive for the pro-inflammatory gene cagA. These novel findings remain to be replicated in larger studies and with the addition of serological determinations to better understand their biological significance in the context of esophageal and gastroesophageal junction cancers.http://link.springer.com/article/10.1186/s13027-019-0233-xHPVHelicobacter pyloriEsophageal cancerEthiopiaCase-control |
| spellingShingle | Maria E. Leon Endale Kassa Abate Bane Tufa Gemechu Yared Tilahun Nigatu Endalafer Sandrine McKay-Chopin Rosario N. Brancaccio Gilles Ferro Mathewos Assefa Elizabeth Ward Massimo Tommasino Abraham Aseffa Joachim Schüz Ahmedin Jemal Tarik Gheit Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia Infectious Agents and Cancer HPV Helicobacter pylori Esophageal cancer Ethiopia Case-control |
| title | Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia |
| title_full | Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia |
| title_fullStr | Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia |
| title_full_unstemmed | Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia |
| title_short | Prevalence of human papillomavirus and Helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case–control study in Ethiopia |
| title_sort | prevalence of human papillomavirus and helicobacter pylori in esophageal and gastroesophageal junction cancer biopsies from a case control study in ethiopia |
| topic | HPV Helicobacter pylori Esophageal cancer Ethiopia Case-control |
| url | http://link.springer.com/article/10.1186/s13027-019-0233-x |
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