Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death
The Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study...
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MDPI AG
2021-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/656 |
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| author | Jie Jiang Guangzhe Yang Yafeng Xin Zhigang Wang Wei Yan Zhufeng Chen Xiaoyan Tang Jixing Xia |
| author_facet | Jie Jiang Guangzhe Yang Yafeng Xin Zhigang Wang Wei Yan Zhufeng Chen Xiaoyan Tang Jixing Xia |
| author_sort | Jie Jiang |
| collection | DOAJ |
| description | The Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study, the rice Mediator subunit 16, <i>OsMed16,</i> was examined. <i>OsMed16</i> encodes a putative protein of 1301 amino acids, which is longer than the version previously reported. It was expressed in various rice organs and localized to the nucleus. The knockout of <i>OsMed16</i> resulted in rice seedling lethality. Its overexpression led to the retardation of rice growth, low yield, and spontaneous cell death in the leaf blade and sheath. RNA sequencing suggested that the overexpression of <i>OsMed16</i> altered the expression of a large number of genes. Among them, the upregulation of some defense-related genes was verified. <i>OsMed16</i> can regulate the expression of a wealth of genes, and alterations in its expression have a profound impact on plant growth, development, and defense responses in rice. |
| first_indexed | 2024-03-10T11:54:28Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:54:28Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-c4b9f07b24c14d60a06be47c7f8dbd6f2023-11-21T17:25:57ZengMDPI AGGenes2073-44252021-04-0112565610.3390/genes12050656Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell DeathJie Jiang0Guangzhe Yang1Yafeng Xin2Zhigang Wang3Wei Yan4Zhufeng Chen5Xiaoyan Tang6Jixing Xia7State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaThe Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study, the rice Mediator subunit 16, <i>OsMed16,</i> was examined. <i>OsMed16</i> encodes a putative protein of 1301 amino acids, which is longer than the version previously reported. It was expressed in various rice organs and localized to the nucleus. The knockout of <i>OsMed16</i> resulted in rice seedling lethality. Its overexpression led to the retardation of rice growth, low yield, and spontaneous cell death in the leaf blade and sheath. RNA sequencing suggested that the overexpression of <i>OsMed16</i> altered the expression of a large number of genes. Among them, the upregulation of some defense-related genes was verified. <i>OsMed16</i> can regulate the expression of a wealth of genes, and alterations in its expression have a profound impact on plant growth, development, and defense responses in rice.https://www.mdpi.com/2073-4425/12/5/656Mediator subunit<i>OsMed16</i><i>Oryza sativa</i>spontaneous cell deathdefense response |
| spellingShingle | Jie Jiang Guangzhe Yang Yafeng Xin Zhigang Wang Wei Yan Zhufeng Chen Xiaoyan Tang Jixing Xia Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death Genes Mediator subunit <i>OsMed16</i> <i>Oryza sativa</i> spontaneous cell death defense response |
| title | Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death |
| title_full | Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death |
| title_fullStr | Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death |
| title_full_unstemmed | Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death |
| title_short | Overexpression of <i>OsMed16</i> Inhibits the Growth of Rice and Causes Spontaneous Cell Death |
| title_sort | overexpression of i osmed16 i inhibits the growth of rice and causes spontaneous cell death |
| topic | Mediator subunit <i>OsMed16</i> <i>Oryza sativa</i> spontaneous cell death defense response |
| url | https://www.mdpi.com/2073-4425/12/5/656 |
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