Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts
In this study, we aimed to evaluate the cellular response of healthy human fibroblasts induced by different types of ultra-low-fluence radiations, including gamma rays, neutrons and high linear energy transfer (LET) heavy ions. NB1RGB cells were pretreated with ultra-low-fluence radiations (~0.1 cGy...
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MDPI AG
2020-08-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/8/154 |
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| author | Masao Suzuki Yukio Uchihori Hisashi Kitamura Masakazu Oikawa Teruaki Konishi |
| author_facet | Masao Suzuki Yukio Uchihori Hisashi Kitamura Masakazu Oikawa Teruaki Konishi |
| author_sort | Masao Suzuki |
| collection | DOAJ |
| description | In this study, we aimed to evaluate the cellular response of healthy human fibroblasts induced by different types of ultra-low-fluence radiations, including gamma rays, neutrons and high linear energy transfer (LET) heavy ions. NB1RGB cells were pretreated with ultra-low-fluence radiations (~0.1 cGy/7–8 h) of <sup>137</sup>Cs gamma rays, <sup>241</sup>Am–Be neutrons, helium, carbon and iron ions before being exposed to an X-ray-challenging dose (1.5 Gy). Helium (LET = 2.3 keV/µm), carbon (LET = 13.3 keV/µm) and iron (LET = 200 keV/µm) ions were generated with the Heavy Ion Medical Accelerator in Chiba (HIMAC), Japan. No differences in cell death—measured by colony-forming assay—were observed regardless of the radiation type applied. In contrast, mutation frequency, which was detected through cell transformation into 6-thioguanine resistant clones, was 1.9 and 4.0 times higher in cells pretreated with helium and carbon ions, respectively, compared to cells exposed to X-ray-challenging dose alone. Moreover, cells pretreated with iron ions or gamma-rays showed a mutation frequency similar to cells exposed to X-ray-challenging dose alone, while cells pretreated with neutrons had 0.15 times less mutations. These results show that cellular responses triggered by ultra-low-fluence irradiations are radiation-quality dependent. Altogether, this study shows that ultra-low-fluence irradiations with the same level as those reported in the International Space Station are capable of inducing different cellular responses, including radio-adaptive responses triggered by neutrons and genomic instability mediated by high-LET heavy ions, while electromagnetic radiations (gamma rays) seem to have no biologic impact. |
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| language | English |
| last_indexed | 2024-03-10T17:15:39Z |
| publishDate | 2020-08-01 |
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| series | Life |
| spelling | doaj.art-c4bb7e451abe4a60b3eaa9ab89ddcfab2023-11-20T10:32:12ZengMDPI AGLife2075-17292020-08-0110815410.3390/life10080154Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human FibroblastsMasao Suzuki0Yukio Uchihori1Hisashi Kitamura2Masakazu Oikawa3Teruaki Konishi4Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Chiba 263-8555, JapanDepartment of Research Planning and Promotion, Quantum Medical Science Directorate, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Chiba 263-8555, JapanDepartment of Radiation Emergency Management, Center for Advanced Radiation Emergency Medicine, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Chiba 263-8555, JapanDepartment of Accelerator and Medical Physics, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Chiba 263-8555, JapanSingle Cell Radiation Biology Group, Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Chiba 263-8555, JapanIn this study, we aimed to evaluate the cellular response of healthy human fibroblasts induced by different types of ultra-low-fluence radiations, including gamma rays, neutrons and high linear energy transfer (LET) heavy ions. NB1RGB cells were pretreated with ultra-low-fluence radiations (~0.1 cGy/7–8 h) of <sup>137</sup>Cs gamma rays, <sup>241</sup>Am–Be neutrons, helium, carbon and iron ions before being exposed to an X-ray-challenging dose (1.5 Gy). Helium (LET = 2.3 keV/µm), carbon (LET = 13.3 keV/µm) and iron (LET = 200 keV/µm) ions were generated with the Heavy Ion Medical Accelerator in Chiba (HIMAC), Japan. No differences in cell death—measured by colony-forming assay—were observed regardless of the radiation type applied. In contrast, mutation frequency, which was detected through cell transformation into 6-thioguanine resistant clones, was 1.9 and 4.0 times higher in cells pretreated with helium and carbon ions, respectively, compared to cells exposed to X-ray-challenging dose alone. Moreover, cells pretreated with iron ions or gamma-rays showed a mutation frequency similar to cells exposed to X-ray-challenging dose alone, while cells pretreated with neutrons had 0.15 times less mutations. These results show that cellular responses triggered by ultra-low-fluence irradiations are radiation-quality dependent. Altogether, this study shows that ultra-low-fluence irradiations with the same level as those reported in the International Space Station are capable of inducing different cellular responses, including radio-adaptive responses triggered by neutrons and genomic instability mediated by high-LET heavy ions, while electromagnetic radiations (gamma rays) seem to have no biologic impact.https://www.mdpi.com/2075-1729/10/8/154ultra-low-fluence irradiationdifferent radiation typeslinear-energy transfercell deathgene mutationbystander effect |
| spellingShingle | Masao Suzuki Yukio Uchihori Hisashi Kitamura Masakazu Oikawa Teruaki Konishi Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts Life ultra-low-fluence irradiation different radiation types linear-energy transfer cell death gene mutation bystander effect |
| title | Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts |
| title_full | Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts |
| title_fullStr | Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts |
| title_full_unstemmed | Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts |
| title_short | Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts |
| title_sort | biologic impact of different ultra low fluence irradiations in human fibroblasts |
| topic | ultra-low-fluence irradiation different radiation types linear-energy transfer cell death gene mutation bystander effect |
| url | https://www.mdpi.com/2075-1729/10/8/154 |
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