Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons
Summary: Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provid...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000320 |
| _version_ | 1818336635860811776 |
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| author | Alessandro Moro Rein I. Hoogstraaten Claudia M. Persoon Matthijs Verhage Ruud F. Toonen |
| author_facet | Alessandro Moro Rein I. Hoogstraaten Claudia M. Persoon Matthijs Verhage Ruud F. Toonen |
| author_sort | Alessandro Moro |
| collection | DOAJ |
| description | Summary: Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles.For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019). |
| first_indexed | 2024-12-13T14:42:28Z |
| format | Article |
| id | doaj.art-c4be119d56b24cecb2bb144800b46d49 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T14:42:28Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c4be119d56b24cecb2bb144800b46d492022-12-21T23:41:35ZengElsevierSTAR Protocols2666-16672021-03-0121100325Quantitative analysis of dense-core vesicle fusion in rodent CNS neuronsAlessandro Moro0Rein I. Hoogstraaten1Claudia M. Persoon2Matthijs Verhage3Ruud F. Toonen4Department of Clinical Genetics, UMC Amsterdam, the NetherlandsDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands; Department of Clinical Genetics, UMC Amsterdam, the NetherlandsDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands; Department of Clinical Genetics, UMC Amsterdam, the NetherlandsDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands; Department of Clinical Genetics, UMC Amsterdam, the NetherlandsDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands; Corresponding authorSummary: Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles.For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166721000320Cell cultureCell membraneMicroscopyNeuroscienceMolecular/chemical probes |
| spellingShingle | Alessandro Moro Rein I. Hoogstraaten Claudia M. Persoon Matthijs Verhage Ruud F. Toonen Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons STAR Protocols Cell culture Cell membrane Microscopy Neuroscience Molecular/chemical probes |
| title | Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons |
| title_full | Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons |
| title_fullStr | Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons |
| title_full_unstemmed | Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons |
| title_short | Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons |
| title_sort | quantitative analysis of dense core vesicle fusion in rodent cns neurons |
| topic | Cell culture Cell membrane Microscopy Neuroscience Molecular/chemical probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000320 |
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