Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli
Watersheds contaminated with municipal, hospital, and agricultural residues are recognized as reservoirs for bacteria carrying antibiotic resistance genes (ARGs). The objective of this study was to determine the potential of environmental bacterial communities from the highly contaminated La Paz Riv...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.997849/full |
| _version_ | 1811245284103028736 |
|---|---|
| author | Jessica Guzman-Otazo Jessica Guzman-Otazo Jessica Guzman-Otazo Enrique Joffré Enrique Joffré Jorge Agramont Nataniel Mamani Jekaterina Jutkina Jekaterina Jutkina Fredrik Boulund Fredrik Boulund Yue O. O. Hu Yue O. O. Hu Daphne Jumilla-Lorenz Anne Farewell Anne Farewell D. G. Joakim Larsson D. G. Joakim Larsson Carl-Fredrik Flach Carl-Fredrik Flach Volga Iñiguez Åsa Sjöling Åsa Sjöling Åsa Sjöling |
| author_facet | Jessica Guzman-Otazo Jessica Guzman-Otazo Jessica Guzman-Otazo Enrique Joffré Enrique Joffré Jorge Agramont Nataniel Mamani Jekaterina Jutkina Jekaterina Jutkina Fredrik Boulund Fredrik Boulund Yue O. O. Hu Yue O. O. Hu Daphne Jumilla-Lorenz Anne Farewell Anne Farewell D. G. Joakim Larsson D. G. Joakim Larsson Carl-Fredrik Flach Carl-Fredrik Flach Volga Iñiguez Åsa Sjöling Åsa Sjöling Åsa Sjöling |
| author_sort | Jessica Guzman-Otazo |
| collection | DOAJ |
| description | Watersheds contaminated with municipal, hospital, and agricultural residues are recognized as reservoirs for bacteria carrying antibiotic resistance genes (ARGs). The objective of this study was to determine the potential of environmental bacterial communities from the highly contaminated La Paz River basin in Bolivia to transfer ARGs to an Escherichia coli lab strain used as the recipient. Additionally, we tested ZnSO4 and CuSO4 at sub-inhibitory concentrations as stressors and analyzed transfer frequencies (TFs), diversity, richness, and acquired resistance profiles. The bacterial communities were collected from surface water in an urban site close to a hospital and near an agricultural area. High transfer potentials of a large set of resistance factors to E. coli were observed at both sites. Whole-genome sequencing revealed that putative plasmids belonging to the incompatibility group N (IncN, IncN2, and IncN3) were predominant among the transconjugants. All IncN variants were verified to be mobile by a second conjugation step. The plasmid backbones were similar to other IncN plasmids isolated worldwide and carried a wide range of ARGs extensively corroborated by phenotypic resistance patterns. Interestingly, all transconjugants also acquired the class 1 integron intl1, which is commonly known as a proxy for anthropogenic pollution. The addition of ZnSO4 and CuSO4 at sub-inhibitory concentrations did not affect the transfer rate. Metal resistance genes were absent from most transconjugants, suggesting a minor role, if any, of metals in the spread of multidrug-resistant plasmids at the investigated sites. |
| first_indexed | 2024-04-12T14:37:25Z |
| format | Article |
| id | doaj.art-c4c1663a62d84a0a9c4ba087578a19c7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T14:37:25Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c4c1663a62d84a0a9c4ba087578a19c72022-12-22T03:29:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.997849997849Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coliJessica Guzman-Otazo0Jessica Guzman-Otazo1Jessica Guzman-Otazo2Enrique Joffré3Enrique Joffré4Jorge Agramont5Nataniel Mamani6Jekaterina Jutkina7Jekaterina Jutkina8Fredrik Boulund9Fredrik Boulund10Yue O. O. Hu11Yue O. O. Hu12Daphne Jumilla-Lorenz13Anne Farewell14Anne Farewell15D. G. Joakim Larsson16D. G. Joakim Larsson17Carl-Fredrik Flach18Carl-Fredrik Flach19Volga Iñiguez20Åsa Sjöling21Åsa Sjöling22Åsa Sjöling23Institute of Molecular Biology and Biotechnology, Universidad Mayor de San Andrés, La Paz, BoliviaDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenCentre for Translational Microbiome Research, Karolinska Institutet, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenCentre for Translational Microbiome Research, Karolinska Institutet, Stockholm, SwedenInstitute of Molecular Biology and Biotechnology, Universidad Mayor de San Andrés, La Paz, BoliviaInstitute of Molecular Biology and Biotechnology, Universidad Mayor de San Andrés, La Paz, BoliviaDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Göteborg, SwedenCentre for Antibiotic Resistance Research (CARe), University of Gothenburg, Göteborg, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenCentre for Translational Microbiome Research, Karolinska Institutet, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenCentre for Translational Microbiome Research, Karolinska Institutet, Stockholm, SwedenDepartment of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, SwedenCentre for Antibiotic Resistance Research (CARe), University of Gothenburg, Göteborg, SwedenDepartment of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, SwedenDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Göteborg, SwedenCentre for Antibiotic Resistance Research (CARe), University of Gothenburg, Göteborg, SwedenDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Göteborg, SwedenCentre for Antibiotic Resistance Research (CARe), University of Gothenburg, Göteborg, SwedenInstitute of Molecular Biology and Biotechnology, Universidad Mayor de San Andrés, La Paz, BoliviaDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenCentre for Translational Microbiome Research, Karolinska Institutet, Stockholm, SwedenDepartment of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, SwedenWatersheds contaminated with municipal, hospital, and agricultural residues are recognized as reservoirs for bacteria carrying antibiotic resistance genes (ARGs). The objective of this study was to determine the potential of environmental bacterial communities from the highly contaminated La Paz River basin in Bolivia to transfer ARGs to an Escherichia coli lab strain used as the recipient. Additionally, we tested ZnSO4 and CuSO4 at sub-inhibitory concentrations as stressors and analyzed transfer frequencies (TFs), diversity, richness, and acquired resistance profiles. The bacterial communities were collected from surface water in an urban site close to a hospital and near an agricultural area. High transfer potentials of a large set of resistance factors to E. coli were observed at both sites. Whole-genome sequencing revealed that putative plasmids belonging to the incompatibility group N (IncN, IncN2, and IncN3) were predominant among the transconjugants. All IncN variants were verified to be mobile by a second conjugation step. The plasmid backbones were similar to other IncN plasmids isolated worldwide and carried a wide range of ARGs extensively corroborated by phenotypic resistance patterns. Interestingly, all transconjugants also acquired the class 1 integron intl1, which is commonly known as a proxy for anthropogenic pollution. The addition of ZnSO4 and CuSO4 at sub-inhibitory concentrations did not affect the transfer rate. Metal resistance genes were absent from most transconjugants, suggesting a minor role, if any, of metals in the spread of multidrug-resistant plasmids at the investigated sites.https://www.frontiersin.org/articles/10.3389/fmicb.2022.997849/fullwaterborne bacteriahorizontal gene transfermulti-drug resistanceEscherichia coliIncN plasmidcopper sulfate |
| spellingShingle | Jessica Guzman-Otazo Jessica Guzman-Otazo Jessica Guzman-Otazo Enrique Joffré Enrique Joffré Jorge Agramont Nataniel Mamani Jekaterina Jutkina Jekaterina Jutkina Fredrik Boulund Fredrik Boulund Yue O. O. Hu Yue O. O. Hu Daphne Jumilla-Lorenz Anne Farewell Anne Farewell D. G. Joakim Larsson D. G. Joakim Larsson Carl-Fredrik Flach Carl-Fredrik Flach Volga Iñiguez Åsa Sjöling Åsa Sjöling Åsa Sjöling Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli Frontiers in Microbiology waterborne bacteria horizontal gene transfer multi-drug resistance Escherichia coli IncN plasmid copper sulfate |
| title | Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli |
| title_full | Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli |
| title_fullStr | Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli |
| title_full_unstemmed | Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli |
| title_short | Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli |
| title_sort | conjugative transfer of multi drug resistance incn plasmids from environmental waterborne bacteria to escherichia coli |
| topic | waterborne bacteria horizontal gene transfer multi-drug resistance Escherichia coli IncN plasmid copper sulfate |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.997849/full |
| work_keys_str_mv | AT jessicaguzmanotazo conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT jessicaguzmanotazo conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT jessicaguzmanotazo conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT enriquejoffre conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT enriquejoffre conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT jorgeagramont conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT natanielmamani conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT jekaterinajutkina conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT jekaterinajutkina conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT fredrikboulund conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT fredrikboulund conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT yueoohu conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT yueoohu conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT daphnejumillalorenz conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT annefarewell conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT annefarewell conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT dgjoakimlarsson conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT dgjoakimlarsson conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT carlfredrikflach conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT carlfredrikflach conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT volgainiguez conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT asasjoling conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT asasjoling conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli AT asasjoling conjugativetransferofmultidrugresistanceincnplasmidsfromenvironmentalwaterbornebacteriatoescherichiacoli |