Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking
The influence of chitinase-3-like protein 1 (YKL-40 or CHI3L1) expression on the immunological properties of the tumor microenvironment, which may affect the effectiveness of immunotherapy, is currently not sufficiently understood in colorectal cancer (CRC). The aim of this study was to investigate...
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MDPI AG
2023-03-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/4/182 |
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| author | Błażej Ochman Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Julia Robotycka Jerzy Piecuch Monika Szrot Sylwia Dzięgielewska-Gęsiak Małgorzata Muc-Wierzgoń Dariusz Waniczek Elżbieta Świętochowska |
| author_facet | Błażej Ochman Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Julia Robotycka Jerzy Piecuch Monika Szrot Sylwia Dzięgielewska-Gęsiak Małgorzata Muc-Wierzgoń Dariusz Waniczek Elżbieta Świętochowska |
| author_sort | Błażej Ochman |
| collection | DOAJ |
| description | The influence of chitinase-3-like protein 1 (YKL-40 or CHI3L1) expression on the immunological properties of the tumor microenvironment, which may affect the effectiveness of immunotherapy, is currently not sufficiently understood in colorectal cancer (CRC). The aim of this study was to investigate the relationship between YKL-40 expression and the immunological properties of the tumor microenvironment in CRC. We performed in silico analysis, including analysis of immune cell infiltration scores and the immune landscape depending on YKL-40 expression, gene set enrichment analysis (GSEA), and analysis of three Gene Expression Omnibus (GEO) datasets. In 48 CRC tissue homogenates and the surgical margin, we analyzed the expression of YKL-40, MMP8, IL17A, and PD-L1. Moreover, we analyzed the expression of YKL-40 in tissue homogenates retrieved from patients with coexisting diabetes, obesity, and smoking. The expression of YKL-40 was significantly higher in CRC tumor tissue compared to healthy tissue and correlated with MMP-8, IL17A, and PD-L1 expression. In silico analysis revealed an association of YKL-40 with disease recurrence, and GSEA revealed a potential link between elevated YKL-40 expression and immunosuppressive properties of the tumor microenvironment in CRC. |
| first_indexed | 2024-03-11T05:08:23Z |
| format | Article |
| id | doaj.art-c4c1d000c33045ff86a57d7f408cc499 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T05:08:23Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-c4c1d000c33045ff86a57d7f408cc4992023-11-17T18:47:26ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-03-014542781279710.3390/cimb45040182Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active SmokingBłażej Ochman0Sylwia Mielcarska1Agnieszka Kula2Miriam Dawidowicz3Julia Robotycka4Jerzy Piecuch5Monika Szrot6Sylwia Dzięgielewska-Gęsiak7Małgorzata Muc-Wierzgoń8Dariusz Waniczek9Elżbieta Świętochowska10Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-800 Zabrze, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-800 Zabrze, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 35 Ceglana, 40-514 Katowice, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 35 Ceglana, 40-514 Katowice, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-800 Zabrze, PolandDepartment of General and Bariatric Surgery and Emergency Medicine in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 10 Marii Curie-Skłodowskiej, 41-800 Zabrze, PolandDepartment of General and Bariatric Surgery and Emergency Medicine in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 10 Marii Curie-Skłodowskiej, 41-800 Zabrze, PolandDepartment of Internal Medicine Prevention in Bytom, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Internal Medicine Prevention in Bytom, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 35 Ceglana, 40-514 Katowice, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-800 Zabrze, PolandThe influence of chitinase-3-like protein 1 (YKL-40 or CHI3L1) expression on the immunological properties of the tumor microenvironment, which may affect the effectiveness of immunotherapy, is currently not sufficiently understood in colorectal cancer (CRC). The aim of this study was to investigate the relationship between YKL-40 expression and the immunological properties of the tumor microenvironment in CRC. We performed in silico analysis, including analysis of immune cell infiltration scores and the immune landscape depending on YKL-40 expression, gene set enrichment analysis (GSEA), and analysis of three Gene Expression Omnibus (GEO) datasets. In 48 CRC tissue homogenates and the surgical margin, we analyzed the expression of YKL-40, MMP8, IL17A, and PD-L1. Moreover, we analyzed the expression of YKL-40 in tissue homogenates retrieved from patients with coexisting diabetes, obesity, and smoking. The expression of YKL-40 was significantly higher in CRC tumor tissue compared to healthy tissue and correlated with MMP-8, IL17A, and PD-L1 expression. In silico analysis revealed an association of YKL-40 with disease recurrence, and GSEA revealed a potential link between elevated YKL-40 expression and immunosuppressive properties of the tumor microenvironment in CRC.https://www.mdpi.com/1467-3045/45/4/182YKL-40 (CHI3L1)PD-L1colorectal cancer |
| spellingShingle | Błażej Ochman Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Julia Robotycka Jerzy Piecuch Monika Szrot Sylwia Dzięgielewska-Gęsiak Małgorzata Muc-Wierzgoń Dariusz Waniczek Elżbieta Świętochowska Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking Current Issues in Molecular Biology YKL-40 (CHI3L1) PD-L1 colorectal cancer |
| title | Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking |
| title_full | Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking |
| title_fullStr | Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking |
| title_full_unstemmed | Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking |
| title_short | Do Elevated YKL-40 Levels Drive the Immunosuppressive Tumor Microenvironment in Colorectal Cancer? Assessment of the Association of the Expression of YKL-40, MMP-8, IL17A, and PD-L1 with Coexisting Type 2 Diabetes, Obesity, and Active Smoking |
| title_sort | do elevated ykl 40 levels drive the immunosuppressive tumor microenvironment in colorectal cancer assessment of the association of the expression of ykl 40 mmp 8 il17a and pd l1 with coexisting type 2 diabetes obesity and active smoking |
| topic | YKL-40 (CHI3L1) PD-L1 colorectal cancer |
| url | https://www.mdpi.com/1467-3045/45/4/182 |
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