Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions
Numerous approaches to cell transplantation of the hepatic or the extrahepatic origin into liver tissue have been developed; however, the efficiency of cell transplantation remains low and liver functions are not well corrected. The liver is a highly immunoreactive organ that contains many resident...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-03-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X640583 |
| _version_ | 1818475141349244928 |
|---|---|
| author | Il-Hwa Hong Seon-Young Han Mi-Ran Ki Young-Mi Moon Jin-Kyu Park Sang-Young You Eun-Mi Lee Ah-Young Kim Eun-Joo Lee Jae-Ho Jeong Kyung-Sun Kang Kyu-Shik Jeong D.V.M., Ph.D. |
| author_facet | Il-Hwa Hong Seon-Young Han Mi-Ran Ki Young-Mi Moon Jin-Kyu Park Sang-Young You Eun-Mi Lee Ah-Young Kim Eun-Joo Lee Jae-Ho Jeong Kyung-Sun Kang Kyu-Shik Jeong D.V.M., Ph.D. |
| author_sort | Il-Hwa Hong |
| collection | DOAJ |
| description | Numerous approaches to cell transplantation of the hepatic or the extrahepatic origin into liver tissue have been developed; however, the efficiency of cell transplantation remains low and liver functions are not well corrected. The liver is a highly immunoreactive organ that contains many resident macrophages known as Kupffer cells. Here, we show that the inhibition of Kupffer cell activity improves stem cell transplantation into liver tissue and corrects some of the liver functions under conditions of liver injury. We found that, when Kupffer cells were inhibited by glycine, numerous adipose-derived stem cells (ASCs) were successfully transplanted into livers, and these transplanted cells showed hepatoprotective effects, including decrease of liver injury factors, increase of liver regeneration, and albumin production. On the contrary, injected ASCs without glycine recruited numerous Kupffer cells, not lymphocytes, and showed low transplantation efficiency. Intriguingly, successfully transplanted ASCs in liver tissue modulated Kupffer cell activity to inhibit tumor necrosis factor-α secretion. Thus, our data show that Kupffer cell inactivation is an important step in order to improve ASC transplantation efficiency and therapeutic potential in liver injuries. In addition, the hepatoprotective function of glycine has synergic effects on liver protection and the engraftment of ASCs. |
| first_indexed | 2024-12-10T09:09:08Z |
| format | Article |
| id | doaj.art-c4c255898b2f49c08de476e43da52be0 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-10T09:09:08Z |
| publishDate | 2013-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-c4c255898b2f49c08de476e43da52be02022-12-22T01:55:05ZengSAGE PublishingCell Transplantation0963-68971555-38922013-03-012210.3727/096368912X640583Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver FunctionsIl-Hwa Hong0Seon-Young Han1Mi-Ran Ki2Young-Mi Moon3Jin-Kyu Park4Sang-Young You5Eun-Mi Lee6Ah-Young Kim7Eun-Joo Lee8Jae-Ho Jeong9Kyung-Sun Kang10Kyu-Shik Jeong D.V.M., Ph.D.11College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaDivision of Plastic Surgery, Yeungnam University Medical Center, Daegu, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaDivision of Plastic Surgery, Yeungnam University Medical Center, Daegu, Republic of KoreaAdult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of KoreaNumerous approaches to cell transplantation of the hepatic or the extrahepatic origin into liver tissue have been developed; however, the efficiency of cell transplantation remains low and liver functions are not well corrected. The liver is a highly immunoreactive organ that contains many resident macrophages known as Kupffer cells. Here, we show that the inhibition of Kupffer cell activity improves stem cell transplantation into liver tissue and corrects some of the liver functions under conditions of liver injury. We found that, when Kupffer cells were inhibited by glycine, numerous adipose-derived stem cells (ASCs) were successfully transplanted into livers, and these transplanted cells showed hepatoprotective effects, including decrease of liver injury factors, increase of liver regeneration, and albumin production. On the contrary, injected ASCs without glycine recruited numerous Kupffer cells, not lymphocytes, and showed low transplantation efficiency. Intriguingly, successfully transplanted ASCs in liver tissue modulated Kupffer cell activity to inhibit tumor necrosis factor-α secretion. Thus, our data show that Kupffer cell inactivation is an important step in order to improve ASC transplantation efficiency and therapeutic potential in liver injuries. In addition, the hepatoprotective function of glycine has synergic effects on liver protection and the engraftment of ASCs.https://doi.org/10.3727/096368912X640583 |
| spellingShingle | Il-Hwa Hong Seon-Young Han Mi-Ran Ki Young-Mi Moon Jin-Kyu Park Sang-Young You Eun-Mi Lee Ah-Young Kim Eun-Joo Lee Jae-Ho Jeong Kyung-Sun Kang Kyu-Shik Jeong D.V.M., Ph.D. Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions Cell Transplantation |
| title | Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions |
| title_full | Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions |
| title_fullStr | Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions |
| title_full_unstemmed | Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions |
| title_short | Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions |
| title_sort | inhibition of kupffer cell activity improves transplantation of human adipose derived stem cells and liver functions |
| url | https://doi.org/10.3727/096368912X640583 |
| work_keys_str_mv | AT ilhwahong inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT seonyounghan inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT miranki inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT youngmimoon inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT jinkyupark inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT sangyoungyou inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT eunmilee inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT ahyoungkim inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT eunjoolee inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT jaehojeong inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT kyungsunkang inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions AT kyushikjeongdvmphd inhibitionofkupffercellactivityimprovestransplantationofhumanadiposederivedstemcellsandliverfunctions |