Innate Lymphoid Cells in the Induction of Obesity
Summary: Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg−/−Rag2−/− mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2−/− mice, which lack only acquired immune ce...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112471930782X |
| _version_ | 1818588067612590080 |
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| author | Takaharu Sasaki Kazuyo Moro Tetsuya Kubota Naoto Kubota Tamotsu Kato Hiroshi Ohno Susumu Nakae Hirohisa Saito Shigeo Koyasu |
| author_facet | Takaharu Sasaki Kazuyo Moro Tetsuya Kubota Naoto Kubota Tamotsu Kato Hiroshi Ohno Susumu Nakae Hirohisa Saito Shigeo Koyasu |
| author_sort | Takaharu Sasaki |
| collection | DOAJ |
| description | Summary: Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg−/−Rag2−/− mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2−/− mice, which lack only acquired immune cells, into Il2rg−/−Rag2−/− mice abolishes this resistance, indicating a role for innate lymphoid cells (ILCs) in this process. Mice lacking ILC2 or ILC3 cells, but not natural killer cells, are resistant to obesity. Adoptive transfer of naive ILC2s isolated from the small intestine (SI), but not ILC2s from white adipose tissue (WAT), restores the induction of diet-induced obesity in Il2rg−/−Rag2−/− mice. Analysis of transcriptional differences reveals that SI-ILC2s express higher levels of IL-2 than do WAT-ILC2s and that blockade of IL-2 signaling impairs weight gain and reduces the populations of ILC2s and ILC3s in the SI, suggesting a role for the IL-2/ILC2/3 axis in the induction of obesity. : Innate lymphoid cells (ILCs) are recently identified lymphocyte populations characterized by the lack of antigen-specific receptors. Sasaki et al. demonstrate the involvement of ILCs in the induction of diet-induced obesity. Specifically, they show that small intestinal ILC2s, but not white adipose tissue ILC2s, are involved in the induction of obesity. Keywords: obesity, high-fat diet, innate lymphoid cells, ILC2, ILC3, small intestine, white adipose tissue, common gamma chain, γc, interleukin-2 |
| first_indexed | 2024-12-16T09:18:52Z |
| format | Article |
| id | doaj.art-c4c68a1ad136451fabc06beafd13a1c0 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-16T09:18:52Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c4c68a1ad136451fabc06beafd13a1c02022-12-21T22:36:50ZengElsevierCell Reports2211-12472019-07-01281202217.e7Innate Lymphoid Cells in the Induction of ObesityTakaharu Sasaki0Kazuyo Moro1Tetsuya Kubota2Naoto Kubota3Tamotsu Kato4Hiroshi Ohno5Susumu Nakae6Hirohisa Saito7Shigeo Koyasu8Laboratory for Innate Immune Systems, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Laboratory for Immune Cell Systems, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanLaboratory for Innate Immune Systems, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Department of Medical Life Science, Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan; Laboratory for Innate Immune Systems, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, Osaka, Japan; Corresponding authorLaboratory for Metabolic Homeostasis, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanLaboratory for Metabolic Homeostasis, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanLaboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Department of Medical Life Science, Graduate School of Medical Life Science, Yokohama City University, Kanagawa, JapanLaboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Department of Medical Life Science, Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan; Intestinal Microbiota Project, Kanagawa Institute of Industrial Science and Technology, Kanagawa, JapanLaboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, JapanDepartment of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, JapanLaboratory for Immune Cell Systems, RIKEN Center for Integrative Medical Sciences, Kanagawa, Japan; Deparment of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan; Corresponding authorSummary: Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg−/−Rag2−/− mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2−/− mice, which lack only acquired immune cells, into Il2rg−/−Rag2−/− mice abolishes this resistance, indicating a role for innate lymphoid cells (ILCs) in this process. Mice lacking ILC2 or ILC3 cells, but not natural killer cells, are resistant to obesity. Adoptive transfer of naive ILC2s isolated from the small intestine (SI), but not ILC2s from white adipose tissue (WAT), restores the induction of diet-induced obesity in Il2rg−/−Rag2−/− mice. Analysis of transcriptional differences reveals that SI-ILC2s express higher levels of IL-2 than do WAT-ILC2s and that blockade of IL-2 signaling impairs weight gain and reduces the populations of ILC2s and ILC3s in the SI, suggesting a role for the IL-2/ILC2/3 axis in the induction of obesity. : Innate lymphoid cells (ILCs) are recently identified lymphocyte populations characterized by the lack of antigen-specific receptors. Sasaki et al. demonstrate the involvement of ILCs in the induction of diet-induced obesity. Specifically, they show that small intestinal ILC2s, but not white adipose tissue ILC2s, are involved in the induction of obesity. Keywords: obesity, high-fat diet, innate lymphoid cells, ILC2, ILC3, small intestine, white adipose tissue, common gamma chain, γc, interleukin-2http://www.sciencedirect.com/science/article/pii/S221112471930782X |
| spellingShingle | Takaharu Sasaki Kazuyo Moro Tetsuya Kubota Naoto Kubota Tamotsu Kato Hiroshi Ohno Susumu Nakae Hirohisa Saito Shigeo Koyasu Innate Lymphoid Cells in the Induction of Obesity Cell Reports |
| title | Innate Lymphoid Cells in the Induction of Obesity |
| title_full | Innate Lymphoid Cells in the Induction of Obesity |
| title_fullStr | Innate Lymphoid Cells in the Induction of Obesity |
| title_full_unstemmed | Innate Lymphoid Cells in the Induction of Obesity |
| title_short | Innate Lymphoid Cells in the Induction of Obesity |
| title_sort | innate lymphoid cells in the induction of obesity |
| url | http://www.sciencedirect.com/science/article/pii/S221112471930782X |
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