Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques
Alcohol misuse and HIV independently induce myopathy. We previously showed that chronic binge alcohol (CBA) administration, with or without simian immunodeficiency virus (SIV), decreases differentiation capacity of male rhesus macaque myoblasts. We hypothesized that short-term alcohol and CBA/SIV wo...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2448 |
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| author | Danielle E. Levitt Brianna L. Bourgeois Keishla M. Rodríguez-Graciani Patricia E. Molina Liz Simon |
| author_facet | Danielle E. Levitt Brianna L. Bourgeois Keishla M. Rodríguez-Graciani Patricia E. Molina Liz Simon |
| author_sort | Danielle E. Levitt |
| collection | DOAJ |
| description | Alcohol misuse and HIV independently induce myopathy. We previously showed that chronic binge alcohol (CBA) administration, with or without simian immunodeficiency virus (SIV), decreases differentiation capacity of male rhesus macaque myoblasts. We hypothesized that short-term alcohol and CBA/SIV would synergistically decrease differentiation capacity and impair bioenergetic parameters in female macaque myoblasts. Myoblasts from naïve (CBA<sup>−</sup>/SIV<sup>−</sup>), vehicle [VEH]/SIV, and CBA/SIV (N = 4–6/group) groups were proliferated (3 days) and differentiated (5 days) with 0 or 50 mM ethanol (short-term). CBA/SIV decreased differentiation and increased non-mitochondrial oxygen consumption rate (OCR) versus naïve and/or VEH/SIV. Short-term alcohol decreased differentiation; increased maximal and non-mitochondrial OCR, mitochondrial reactive oxygen species (ROS) production, and aldolase activity; and decreased glycolytic measures, ATP production, mitochondrial membrane potential (ΔΨm), and pyruvate kinase activity. Mitochondrial ROS production was closely associated with mitochondrial network volume, and differentiation indices were closely associated with key bioenergetic health and function parameters. Results indicate that short-term alcohol and CBA non-synergistically decrease myoblast differentiation capacity. Short-term alcohol impaired myoblast glycolytic function, driving the bioenergetic deficit. Results suggest potentially differing mechanisms underlying decreased differentiation capacity with short-term alcohol and CBA, highlighting the need to elucidate the impact of different alcohol use patterns on myopathy. |
| first_indexed | 2024-03-07T22:29:03Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:29:03Z |
| publishDate | 2024-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4c80f6b325b4c2f811ca11e580927c12024-02-23T15:21:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254244810.3390/ijms25042448Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female MacaquesDanielle E. Levitt0Brianna L. Bourgeois1Keishla M. Rodríguez-Graciani2Patricia E. Molina3Liz Simon4Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USADepartment of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USADepartment of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USADepartment of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USADepartment of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USAAlcohol misuse and HIV independently induce myopathy. We previously showed that chronic binge alcohol (CBA) administration, with or without simian immunodeficiency virus (SIV), decreases differentiation capacity of male rhesus macaque myoblasts. We hypothesized that short-term alcohol and CBA/SIV would synergistically decrease differentiation capacity and impair bioenergetic parameters in female macaque myoblasts. Myoblasts from naïve (CBA<sup>−</sup>/SIV<sup>−</sup>), vehicle [VEH]/SIV, and CBA/SIV (N = 4–6/group) groups were proliferated (3 days) and differentiated (5 days) with 0 or 50 mM ethanol (short-term). CBA/SIV decreased differentiation and increased non-mitochondrial oxygen consumption rate (OCR) versus naïve and/or VEH/SIV. Short-term alcohol decreased differentiation; increased maximal and non-mitochondrial OCR, mitochondrial reactive oxygen species (ROS) production, and aldolase activity; and decreased glycolytic measures, ATP production, mitochondrial membrane potential (ΔΨm), and pyruvate kinase activity. Mitochondrial ROS production was closely associated with mitochondrial network volume, and differentiation indices were closely associated with key bioenergetic health and function parameters. Results indicate that short-term alcohol and CBA non-synergistically decrease myoblast differentiation capacity. Short-term alcohol impaired myoblast glycolytic function, driving the bioenergetic deficit. Results suggest potentially differing mechanisms underlying decreased differentiation capacity with short-term alcohol and CBA, highlighting the need to elucidate the impact of different alcohol use patterns on myopathy.https://www.mdpi.com/1422-0067/25/4/2448alcohol use disorderglycolysismitochondriaethanolantiretroviral therapyHIV |
| spellingShingle | Danielle E. Levitt Brianna L. Bourgeois Keishla M. Rodríguez-Graciani Patricia E. Molina Liz Simon Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques International Journal of Molecular Sciences alcohol use disorder glycolysis mitochondria ethanol antiretroviral therapy HIV |
| title | Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques |
| title_full | Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques |
| title_fullStr | Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques |
| title_full_unstemmed | Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques |
| title_short | Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques |
| title_sort | alcohol impairs bioenergetics and differentiation capacity of myoblasts from simian immunodeficiency virus infected female macaques |
| topic | alcohol use disorder glycolysis mitochondria ethanol antiretroviral therapy HIV |
| url | https://www.mdpi.com/1422-0067/25/4/2448 |
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