Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract
The present study was designed to evaluate the chemical extraction, chemical composition, and antioxidant and antibacterial properties of the total flavonoids in Willow Buds (TFW). We investigated the optimal extraction of TFW using response surface methodology (RSM). Chemical compounds were analyze...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/17/5695 |
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| author | Peng Zhang Yuwen Song Hongling Wang Yujie Fu Yingying Zhang Korotkova Irina Pavlovna |
| author_facet | Peng Zhang Yuwen Song Hongling Wang Yujie Fu Yingying Zhang Korotkova Irina Pavlovna |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | The present study was designed to evaluate the chemical extraction, chemical composition, and antioxidant and antibacterial properties of the total flavonoids in Willow Buds (TFW). We investigated the optimal extraction of TFW using response surface methodology (RSM). Chemical compounds were analyzed using Q-Orbitrap LC–MS/MS. The DPPH radical scavenging capacity, hydroxy radical inhibitory ability, and superoxide anion radical inhibitory ability were explored to determine the antioxidant properties of flavonoid extractions. The antibacterial effect was assessed via minimal inhibitory concentration. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 50%, a time of 35 min, and a liquid/material ratio of 70:1 mL/g. Under these conditions, the yield of TFW was 7.57%. Eight flavonoids, a phenolic glycoside, and an alkaloid were enriched in the Willow Buds. The TFW exhibited significant antioxidant activity, with IC<sub>50</sub> values of 0.18-0.24 mg/mL and antimicrobial activity against <i>Escherichia coli</i>, <i>Salmonella enterica</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus pneumoniae</i>. TFW may be explored as potential and natural compounds in food and pharmacological applications. |
| first_indexed | 2024-03-10T01:29:04Z |
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| id | doaj.art-c4c90f8b4ae7405a9ce9b47ed41db7a2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:29:04Z |
| publishDate | 2022-09-01 |
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| series | Molecules |
| spelling | doaj.art-c4c90f8b4ae7405a9ce9b47ed41db7a22023-11-23T13:46:32ZengMDPI AGMolecules1420-30492022-09-012717569510.3390/molecules27175695Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the ExtractPeng Zhang0Yuwen Song1Hongling Wang2Yujie Fu3Yingying Zhang4Korotkova Irina Pavlovna5College of Life Engineering, Shenyang Institute of Technology, Fushun 113122, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Life Engineering, Shenyang Institute of Technology, Fushun 113122, ChinaCollege of Life Engineering, Shenyang Institute of Technology, Fushun 113122, ChinaCollege of Life Engineering, Shenyang Institute of Technology, Fushun 113122, ChinaPrimorskaya State Academy of Agriculture, Ussuriisk 692510, RussiaThe present study was designed to evaluate the chemical extraction, chemical composition, and antioxidant and antibacterial properties of the total flavonoids in Willow Buds (TFW). We investigated the optimal extraction of TFW using response surface methodology (RSM). Chemical compounds were analyzed using Q-Orbitrap LC–MS/MS. The DPPH radical scavenging capacity, hydroxy radical inhibitory ability, and superoxide anion radical inhibitory ability were explored to determine the antioxidant properties of flavonoid extractions. The antibacterial effect was assessed via minimal inhibitory concentration. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 50%, a time of 35 min, and a liquid/material ratio of 70:1 mL/g. Under these conditions, the yield of TFW was 7.57%. Eight flavonoids, a phenolic glycoside, and an alkaloid were enriched in the Willow Buds. The TFW exhibited significant antioxidant activity, with IC<sub>50</sub> values of 0.18-0.24 mg/mL and antimicrobial activity against <i>Escherichia coli</i>, <i>Salmonella enterica</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus pneumoniae</i>. TFW may be explored as potential and natural compounds in food and pharmacological applications.https://www.mdpi.com/1420-3049/27/17/5695Willow Budstotal flavonoidsextraction technologyantibacterialantioxidationchemical component |
| spellingShingle | Peng Zhang Yuwen Song Hongling Wang Yujie Fu Yingying Zhang Korotkova Irina Pavlovna Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract Molecules Willow Buds total flavonoids extraction technology antibacterial antioxidation chemical component |
| title | Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract |
| title_full | Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract |
| title_fullStr | Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract |
| title_full_unstemmed | Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract |
| title_short | Optimization of Flavonoid Extraction from <i>Salix babylonica L.</i> Buds, and the Antioxidant and Antibacterial Activities of the Extract |
| title_sort | optimization of flavonoid extraction from i salix babylonica l i buds and the antioxidant and antibacterial activities of the extract |
| topic | Willow Buds total flavonoids extraction technology antibacterial antioxidation chemical component |
| url | https://www.mdpi.com/1420-3049/27/17/5695 |
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