Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil
Introduction: In vitro antioxidant and antibacterial activity and volatile compositions of two Heracleum species (Apiaceae) including Heracleum transcaucasicum and Heracleum anisactis roots Essential Oil (EO) were investigated.Methods: The volatile compositions of EOs were analyzed by GC/Mass...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2014-06-01
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| Series: | BioImpacts |
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| Online Access: | http://journals.tbzmed.ac.ir/BI/PDF/BI-4-69.pdf |
| _version_ | 1818550569249275904 |
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| author | Torbati Mohammadali Nazemiyeh Hossein Lotfipour Farzaneh Nemati Mahboob Asnaashari Solmaz Fathiazad Fatemeh |
| author_facet | Torbati Mohammadali Nazemiyeh Hossein Lotfipour Farzaneh Nemati Mahboob Asnaashari Solmaz Fathiazad Fatemeh |
| author_sort | Torbati Mohammadali |
| collection | DOAJ |
| description | Introduction:
In vitro antioxidant and antibacterial activity and volatile compositions of two Heracleum species (Apiaceae) including Heracleum transcaucasicum and Heracleum anisactis roots Essential Oil (EO) were investigated.Methods: The volatile compositions of EOs were analyzed by GC/Mass spectroscopy. To detect the antioxidant activity of essential oils TLC-bioautography and DPPH radical scavenging assay by spectrophotometry was performed. Additionally, the antibacterial activity of two essential oils were studied and compared against four pathogenic bacteria by agar disc diffusion method and MIC values of the EOs were determined using the broth dilution method.Results: Myristicin was the dominant component in both EOs. It was identified as 96.87% and 95.15% of the essential oil composition of H. transcaucasicum and H. anisactis roots, respectively. The TLC-bioautography showed antioxidant spots in both EOs and IC50 of H. anisactis and H. transcaucasicum EO was found to be 54 µg × ml (-1) and 77 µg × ml (-1), respectively. Regarding the antimicrobial assay, H. anisactis EO exhibited weak to moderate antibacterial activity against gram-positive bacteria and also Escherichia coli, whereas the essential oil from H. transcaucasicum was inactive.Conclusion: Based on the results from this study, both tested EOs mainly consist of myristicin. Despite the presence of myristicin with known antibacterial property, the EO from H. transcacausicum showed no antibacterial activity. Thus it is supposed that the biological activity of plants is remarkably linked to the extracts’ chemical profile and intercomponents’ synergistic or antagonistic effect could play a crucial role in bioactivity of EOs and other plant extracts. |
| first_indexed | 2024-12-12T08:48:13Z |
| format | Article |
| id | doaj.art-c4cb1240ea59439f82524e212659525d |
| institution | Directory Open Access Journal |
| issn | 2228-5652 2228-5660 |
| language | English |
| last_indexed | 2024-12-12T08:48:13Z |
| publishDate | 2014-06-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-c4cb1240ea59439f82524e212659525d2022-12-22T00:30:21ZengTabriz University of Medical SciencesBioImpacts2228-56522228-56602014-06-0142697410.5681/bi.2014.004BI_1142_20130902143539Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oilTorbati Mohammadali0Nazemiyeh Hossein1Lotfipour Farzaneh2Nemati Mahboob3Asnaashari Solmaz4Fathiazad Fatemeh5Students’ Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranPharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranPharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranIntroduction: In vitro antioxidant and antibacterial activity and volatile compositions of two Heracleum species (Apiaceae) including Heracleum transcaucasicum and Heracleum anisactis roots Essential Oil (EO) were investigated.Methods: The volatile compositions of EOs were analyzed by GC/Mass spectroscopy. To detect the antioxidant activity of essential oils TLC-bioautography and DPPH radical scavenging assay by spectrophotometry was performed. Additionally, the antibacterial activity of two essential oils were studied and compared against four pathogenic bacteria by agar disc diffusion method and MIC values of the EOs were determined using the broth dilution method.Results: Myristicin was the dominant component in both EOs. It was identified as 96.87% and 95.15% of the essential oil composition of H. transcaucasicum and H. anisactis roots, respectively. The TLC-bioautography showed antioxidant spots in both EOs and IC50 of H. anisactis and H. transcaucasicum EO was found to be 54 µg × ml (-1) and 77 µg × ml (-1), respectively. Regarding the antimicrobial assay, H. anisactis EO exhibited weak to moderate antibacterial activity against gram-positive bacteria and also Escherichia coli, whereas the essential oil from H. transcaucasicum was inactive.Conclusion: Based on the results from this study, both tested EOs mainly consist of myristicin. Despite the presence of myristicin with known antibacterial property, the EO from H. transcacausicum showed no antibacterial activity. Thus it is supposed that the biological activity of plants is remarkably linked to the extracts’ chemical profile and intercomponents’ synergistic or antagonistic effect could play a crucial role in bioactivity of EOs and other plant extracts.http://journals.tbzmed.ac.ir/BI/PDF/BI-4-69.pdfTLC-bioautographyHeracleum anisactisHeracleum transcaucasicumGC/MS SpectrometryMyristcinEugenol |
| spellingShingle | Torbati Mohammadali Nazemiyeh Hossein Lotfipour Farzaneh Nemati Mahboob Asnaashari Solmaz Fathiazad Fatemeh Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil BioImpacts TLC-bioautography Heracleum anisactis Heracleum transcaucasicum GC/MS Spectrometry Myristcin Eugenol |
| title | Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil |
| title_full | Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil |
| title_fullStr | Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil |
| title_full_unstemmed | Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil |
| title_short | Chemical composition and in vitro antioxidant and antibacterial activity of Heracleum transcaucasicum and Heracleum anisactis roots essential oil |
| title_sort | chemical composition and in vitro antioxidant and antibacterial activity of heracleum transcaucasicum and heracleum anisactis roots essential oil |
| topic | TLC-bioautography Heracleum anisactis Heracleum transcaucasicum GC/MS Spectrometry Myristcin Eugenol |
| url | http://journals.tbzmed.ac.ir/BI/PDF/BI-4-69.pdf |
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