Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model
The current study was designed to investigate the protective role of diosmin against cyclophosphamide-induced premature ovarian insufficiency (POI). Female Swiss albino rats received a single intraperitoneal dose of cyclophosphamide (200 mg/kg) followed by 8 mg/kg/day for the next 15 consecutive day...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/3044 |
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| author | Noha M. Abogresha Sally S. Mohammed Marwa M. Hosny Hoda Y. Abdallah Ahmed M. Gadallah Sahar M. Greish |
| author_facet | Noha M. Abogresha Sally S. Mohammed Marwa M. Hosny Hoda Y. Abdallah Ahmed M. Gadallah Sahar M. Greish |
| author_sort | Noha M. Abogresha |
| collection | DOAJ |
| description | The current study was designed to investigate the protective role of diosmin against cyclophosphamide-induced premature ovarian insufficiency (POI). Female Swiss albino rats received a single intraperitoneal dose of cyclophosphamide (200 mg/kg) followed by 8 mg/kg/day for the next 15 consecutive days either alone or in combination with oral diosmin at 50 or 100 mg/kg. Histopathological examination of ovarian tissues, hormonal assays for follicle stimulating hormone (FSH), estradiol (E2), and anti-Mullerian hormone (AMH), assessment of the oxidative stress status, as well as measurement of the relative expression of miRNA-145 and its target genes [vascular endothelial growth factor B <i>(VEGF-B)</i> and regulator of cell cycle (<i>RGC32</i>)] were performed. Diosmin treatment ameliorated the levels of E2, AMH, and oxidative stress markers. Additionally, both low and high diosmin doses significantly reduced the histopathological alterations and nearly preserved the normal ovarian reserve. MiRNA-145 expression was upregulated after treatment with diosmin high dose. miRNA-145 target genes were over-expressed after both low and high diosmin administration. Based on our findings, diosmin has a dose-dependent protective effect against cyclophosphamide-induced ovarian toxicity in rats. |
| first_indexed | 2024-03-10T13:11:05Z |
| format | Article |
| id | doaj.art-c4cd50db5c6049fca5ae634f73a5c86b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:11:05Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4cd50db5c6049fca5ae634f73a5c86b2023-11-21T10:47:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226304410.3390/ijms22063044Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat ModelNoha M. Abogresha0Sally S. Mohammed1Marwa M. Hosny2Hoda Y. Abdallah3Ahmed M. Gadallah4Sahar M. Greish5Physiology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptHistology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Histology and Cell Biology (Genetics Unit), Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptObstetrics and Gynecology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptPhysiology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptThe current study was designed to investigate the protective role of diosmin against cyclophosphamide-induced premature ovarian insufficiency (POI). Female Swiss albino rats received a single intraperitoneal dose of cyclophosphamide (200 mg/kg) followed by 8 mg/kg/day for the next 15 consecutive days either alone or in combination with oral diosmin at 50 or 100 mg/kg. Histopathological examination of ovarian tissues, hormonal assays for follicle stimulating hormone (FSH), estradiol (E2), and anti-Mullerian hormone (AMH), assessment of the oxidative stress status, as well as measurement of the relative expression of miRNA-145 and its target genes [vascular endothelial growth factor B <i>(VEGF-B)</i> and regulator of cell cycle (<i>RGC32</i>)] were performed. Diosmin treatment ameliorated the levels of E2, AMH, and oxidative stress markers. Additionally, both low and high diosmin doses significantly reduced the histopathological alterations and nearly preserved the normal ovarian reserve. MiRNA-145 expression was upregulated after treatment with diosmin high dose. miRNA-145 target genes were over-expressed after both low and high diosmin administration. Based on our findings, diosmin has a dose-dependent protective effect against cyclophosphamide-induced ovarian toxicity in rats.https://www.mdpi.com/1422-0067/22/6/3044premature ovarian insufficiencymiRNA-145oxidative stress |
| spellingShingle | Noha M. Abogresha Sally S. Mohammed Marwa M. Hosny Hoda Y. Abdallah Ahmed M. Gadallah Sahar M. Greish Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model International Journal of Molecular Sciences premature ovarian insufficiency miRNA-145 oxidative stress |
| title | Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model |
| title_full | Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model |
| title_fullStr | Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model |
| title_full_unstemmed | Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model |
| title_short | Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model |
| title_sort | diosmin mitigates cyclophosphamide induced premature ovarian insufficiency in rat model |
| topic | premature ovarian insufficiency miRNA-145 oxidative stress |
| url | https://www.mdpi.com/1422-0067/22/6/3044 |
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