Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm
Background: Candida albicans (C. albicans) are dimorphic fungi in oral cavity, considered not only as normal flora, but also as pathogens. C. albicans have an ability to grow biofilm, which has a thick layer of outer skin structure, called as extracellular matrix. Jengkol leaves (Pithecellobium jiri...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2016-09-01
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| Series: | Dental Journal |
| Subjects: | |
| Online Access: | http://e-journal.unair.ac.id/index.php/MKG/article/view/3210 |
| _version_ | 1819003516727853056 |
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| author | Muhammad Luthfi Ira Arundina Nizamiar Hamni |
| author_facet | Muhammad Luthfi Ira Arundina Nizamiar Hamni |
| author_sort | Muhammad Luthfi |
| collection | DOAJ |
| description | Background: Candida albicans (C. albicans) are dimorphic fungi in oral cavity, considered not only as normal flora, but also as pathogens. C. albicans have an ability to grow biofilm, which has a thick layer of outer skin structure, called as extracellular matrix. Jengkol leaves (Pithecellobium jiringa) contain alkaloids, flavonoids, terpenoids, and lectins, which have an ability as antifungal agent Purpose: This study aimed to analyze the optimum dose of jengkol leaf extract as antibiofilm against C. albicans biofilms. Method: C. albicans were cultured on yeast peptone dextrosa (YPD) media in 96-well microtiter plate flat bottom plates. There were one control group (without treatment) and three treatment groups. The first treatment group was given jengkol leaf extract at a dose of 100 mg/ ml. The second treatment group was given jengkol leaf extract at a dose of 200 mg/ ml. And, the third treatment group was given jengkol leaf extract at a dose of 400 mg/ ml. Semi quantitative method was applied to determine C. albicans biofilmsis using Crystal Violet staining technique. The absorbance of the cells then was calculated using a spectrophotometer with a wavelength of 570 nm. Result: The mean value of optical density in the control group was 1.23. The mean value of optical density in the treatment group with a dose of 100 mg/ ml was 0.2. Meanwhile, the mean value of optical density in the treatment group with a dose of 200 mg/ ml was 0.2, and 0.21 in the treatment group with a dose of 400 mg/ ml. The results also showed that there were significant differences between the control group and all of the treatment groups (p<0.05), but there was no significant difference between the treatment groups (p>0.05). Conclusion: The optimum dose of jengkol leaf extract used as antibiofilm against C. albicans biofilms is 100 mg /ml with an inhibitory percentage of 83.7%. |
| first_indexed | 2024-12-20T23:22:15Z |
| format | Article |
| id | doaj.art-c4cf7c752be947be919ca54f1f379aba |
| institution | Directory Open Access Journal |
| issn | 1978-3728 2442-9740 |
| language | English |
| last_indexed | 2024-12-20T23:22:15Z |
| publishDate | 2016-09-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj.art-c4cf7c752be947be919ca54f1f379aba2022-12-21T19:23:29ZengUniversitas AirlanggaDental Journal1978-37282442-97402016-09-0149314815210.20473/j.djmkg.v49.i3.p148-1522458Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilmMuhammad Luthfi0Ira Arundina1Nizamiar Hamni2Faculty of Dental Medicine, Universitas Airlangga, SurabayaFaculty of Dental Medicine, Universitas Airlangga, SurabayaFaculty of Dental Medicine, Universitas Airlangga, SurabayaBackground: Candida albicans (C. albicans) are dimorphic fungi in oral cavity, considered not only as normal flora, but also as pathogens. C. albicans have an ability to grow biofilm, which has a thick layer of outer skin structure, called as extracellular matrix. Jengkol leaves (Pithecellobium jiringa) contain alkaloids, flavonoids, terpenoids, and lectins, which have an ability as antifungal agent Purpose: This study aimed to analyze the optimum dose of jengkol leaf extract as antibiofilm against C. albicans biofilms. Method: C. albicans were cultured on yeast peptone dextrosa (YPD) media in 96-well microtiter plate flat bottom plates. There were one control group (without treatment) and three treatment groups. The first treatment group was given jengkol leaf extract at a dose of 100 mg/ ml. The second treatment group was given jengkol leaf extract at a dose of 200 mg/ ml. And, the third treatment group was given jengkol leaf extract at a dose of 400 mg/ ml. Semi quantitative method was applied to determine C. albicans biofilmsis using Crystal Violet staining technique. The absorbance of the cells then was calculated using a spectrophotometer with a wavelength of 570 nm. Result: The mean value of optical density in the control group was 1.23. The mean value of optical density in the treatment group with a dose of 100 mg/ ml was 0.2. Meanwhile, the mean value of optical density in the treatment group with a dose of 200 mg/ ml was 0.2, and 0.21 in the treatment group with a dose of 400 mg/ ml. The results also showed that there were significant differences between the control group and all of the treatment groups (p<0.05), but there was no significant difference between the treatment groups (p>0.05). Conclusion: The optimum dose of jengkol leaf extract used as antibiofilm against C. albicans biofilms is 100 mg /ml with an inhibitory percentage of 83.7%.http://e-journal.unair.ac.id/index.php/MKG/article/view/3210jengkol leaf extractantibiofilmCandida albicans |
| spellingShingle | Muhammad Luthfi Ira Arundina Nizamiar Hamni Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm Dental Journal jengkol leaf extract antibiofilm Candida albicans |
| title | Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm |
| title_full | Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm |
| title_fullStr | Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm |
| title_full_unstemmed | Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm |
| title_short | Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm |
| title_sort | inhibitory effect of jengkol leaf pithecellobium jiringa extract to inhibit candida albicans biofilm |
| topic | jengkol leaf extract antibiofilm Candida albicans |
| url | http://e-journal.unair.ac.id/index.php/MKG/article/view/3210 |
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