Serological determinants of COVID-19
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection spreaded rapidly worldwide, as far as it has become a global pandemic. Therefore, the introduction of serological tests for determination of IgM and IgG antibodies has become the main diagnostic tool, useful f...
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| Format: | Article |
| Language: | English |
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BMC
2020-11-01
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| Series: | Biology Direct |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13062-020-00276-1 |
| _version_ | 1818458263174250496 |
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| author | Annalisa Noce Maria Luisa Santoro Giulia Marrone Cartesio D’Agostini Ivano Amelio Andrea Duggento Manfredi Tesauro Nicola Di Daniele |
| author_facet | Annalisa Noce Maria Luisa Santoro Giulia Marrone Cartesio D’Agostini Ivano Amelio Andrea Duggento Manfredi Tesauro Nicola Di Daniele |
| author_sort | Annalisa Noce |
| collection | DOAJ |
| description | Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection spreaded rapidly worldwide, as far as it has become a global pandemic. Therefore, the introduction of serological tests for determination of IgM and IgG antibodies has become the main diagnostic tool, useful for tracking the spread of the virus and for consequently allowing its containment. In our study we compared point of care test (POCT) lateral flow immunoassay (FIA) vs automated chemiluminescent immunoassay (CLIA), in order to assess their specificity and sensibility for COVID-19 antibodies detection. Results We find that different specificities and sensitivities for IgM and IgG tests. Notably IgM POCT FIA method vs CLIA method (gold standard) has a low sensitivity (0.526), while IgG POCT FIA method vs CLIA method (gold standard) test has a much higher sensitivity (0.937); further, with respect of IgG, FIA and CLIA could arguably provide equivalent information. Conclusions FIA method could be helpful in assessing in short time, the possible contagiousness of subjects that for work reasons cannot guarantee “social distancing”. |
| first_indexed | 2024-12-14T22:55:41Z |
| format | Article |
| id | doaj.art-c4cf8cd3b66546c0a96ef9108b40aa5f |
| institution | Directory Open Access Journal |
| issn | 1745-6150 |
| language | English |
| last_indexed | 2024-12-14T22:55:41Z |
| publishDate | 2020-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biology Direct |
| spelling | doaj.art-c4cf8cd3b66546c0a96ef9108b40aa5f2022-12-21T22:44:36ZengBMCBiology Direct1745-61502020-11-011511910.1186/s13062-020-00276-1Serological determinants of COVID-19Annalisa Noce0Maria Luisa Santoro1Giulia Marrone2Cartesio D’Agostini3Ivano Amelio4Andrea Duggento5Manfredi Tesauro6Nicola Di Daniele7UOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor VergataLaboratory Pathologist Director of Artemisia Lab – AlessandriaUOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor VergataDepartment of Experimental Medicine, University of Rome Tor VergataDepartment of Experimental Medicine, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataUOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor VergataUOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor VergataAbstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection spreaded rapidly worldwide, as far as it has become a global pandemic. Therefore, the introduction of serological tests for determination of IgM and IgG antibodies has become the main diagnostic tool, useful for tracking the spread of the virus and for consequently allowing its containment. In our study we compared point of care test (POCT) lateral flow immunoassay (FIA) vs automated chemiluminescent immunoassay (CLIA), in order to assess their specificity and sensibility for COVID-19 antibodies detection. Results We find that different specificities and sensitivities for IgM and IgG tests. Notably IgM POCT FIA method vs CLIA method (gold standard) has a low sensitivity (0.526), while IgG POCT FIA method vs CLIA method (gold standard) test has a much higher sensitivity (0.937); further, with respect of IgG, FIA and CLIA could arguably provide equivalent information. Conclusions FIA method could be helpful in assessing in short time, the possible contagiousness of subjects that for work reasons cannot guarantee “social distancing”.http://link.springer.com/article/10.1186/s13062-020-00276-1SARS-CoV-2COVID-19Point of careLateral flow immunoassayAutomated chemiluminescent immunoassaySerological tests |
| spellingShingle | Annalisa Noce Maria Luisa Santoro Giulia Marrone Cartesio D’Agostini Ivano Amelio Andrea Duggento Manfredi Tesauro Nicola Di Daniele Serological determinants of COVID-19 Biology Direct SARS-CoV-2 COVID-19 Point of care Lateral flow immunoassay Automated chemiluminescent immunoassay Serological tests |
| title | Serological determinants of COVID-19 |
| title_full | Serological determinants of COVID-19 |
| title_fullStr | Serological determinants of COVID-19 |
| title_full_unstemmed | Serological determinants of COVID-19 |
| title_short | Serological determinants of COVID-19 |
| title_sort | serological determinants of covid 19 |
| topic | SARS-CoV-2 COVID-19 Point of care Lateral flow immunoassay Automated chemiluminescent immunoassay Serological tests |
| url | http://link.springer.com/article/10.1186/s13062-020-00276-1 |
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