Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models
As a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane li...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/5932 |
| _version_ | 1797493259478499328 |
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| author | Zahra Nasri Mohsen Ahmadi Johanna Striesow Mehdi Ravandeh Thomas von Woedtke Kristian Wende |
| author_facet | Zahra Nasri Mohsen Ahmadi Johanna Striesow Mehdi Ravandeh Thomas von Woedtke Kristian Wende |
| author_sort | Zahra Nasri |
| collection | DOAJ |
| description | As a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane lipids and properties is not fully understood. For this purpose, the changes in the lipid bilayer functionality under oxidative stress generated by an argon plasma jet (kINPen) were investigated by electrochemical techniques. In addition, liquid chromatography-tandem mass spectrometry was employed to analyze the plasma-induced modifications on the model lipids. Various asymmetric bilayers mimicking the structure and properties of the erythrocyte cell membrane were transferred onto a gold electrode surface by Langmuir-Blodgett/Langmuir-Schaefer deposition techniques. A strong impact of cholesterol on membrane permeabilization by plasma-derived species was revealed. Moreover, the maintenance of the barrier properties is influenced by the chemical composition of the head group. Mainly the head group size and its hydrogen bonding capacities are relevant, and phosphatidylcholines are significantly more susceptible than phosphatidylserines and other lipid classes, underlining the high relevance of this lipid class in membrane dynamics and cell physiology. |
| first_indexed | 2024-03-10T01:17:27Z |
| format | Article |
| id | doaj.art-c4d0579d05af44e68c25b2147b609e13 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:17:27Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4d0579d05af44e68c25b2147b609e132023-11-23T14:06:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311593210.3390/ijms23115932Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer ModelsZahra Nasri0Mohsen Ahmadi1Johanna Striesow2Mehdi Ravandeh3Thomas von Woedtke4Kristian Wende5Center for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyCenter for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyCenter for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyCenter for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyCenter for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyCenter for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyAs a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane lipids and properties is not fully understood. For this purpose, the changes in the lipid bilayer functionality under oxidative stress generated by an argon plasma jet (kINPen) were investigated by electrochemical techniques. In addition, liquid chromatography-tandem mass spectrometry was employed to analyze the plasma-induced modifications on the model lipids. Various asymmetric bilayers mimicking the structure and properties of the erythrocyte cell membrane were transferred onto a gold electrode surface by Langmuir-Blodgett/Langmuir-Schaefer deposition techniques. A strong impact of cholesterol on membrane permeabilization by plasma-derived species was revealed. Moreover, the maintenance of the barrier properties is influenced by the chemical composition of the head group. Mainly the head group size and its hydrogen bonding capacities are relevant, and phosphatidylcholines are significantly more susceptible than phosphatidylserines and other lipid classes, underlining the high relevance of this lipid class in membrane dynamics and cell physiology.https://www.mdpi.com/1422-0067/23/11/5932reactive oxygen and nitrogen speciescold physical plasmacell membranesupported lipid bilayerselectrochemistryLangmuir-Blodgett and Schaefer techniques |
| spellingShingle | Zahra Nasri Mohsen Ahmadi Johanna Striesow Mehdi Ravandeh Thomas von Woedtke Kristian Wende Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models International Journal of Molecular Sciences reactive oxygen and nitrogen species cold physical plasma cell membrane supported lipid bilayers electrochemistry Langmuir-Blodgett and Schaefer techniques |
| title | Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models |
| title_full | Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models |
| title_fullStr | Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models |
| title_full_unstemmed | Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models |
| title_short | Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models |
| title_sort | insight into the impact of oxidative stress on the barrier properties of lipid bilayer models |
| topic | reactive oxygen and nitrogen species cold physical plasma cell membrane supported lipid bilayers electrochemistry Langmuir-Blodgett and Schaefer techniques |
| url | https://www.mdpi.com/1422-0067/23/11/5932 |
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