Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice
Loss of immunity is an important cause in the pathology of infectious disease. This study investigates the effect of <i>Litsea japonica</i> fruit extract (LJFE) as a potential functional food on immunity and hematopoietic function in immunosuppressed BALB/c mice. Immunity-stimulating act...
| Κύριοι συγγραφείς: | , , , , , , , , , , , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | English |
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MDPI AG
2022-12-01
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| Σειρά: | Applied Sciences |
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| Διαθέσιμο Online: | https://www.mdpi.com/2076-3417/13/1/145 |
| _version_ | 1827761205289156608 |
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| author | Seon Yeong Ji EunJin Bang Hyun Hwangbo Min Yeong Kim Da Hye Kim Young Tae Koo Jin Soo Kim Ki Won Lee Sun Young Park Chan-Young Kwon Hyesook Lee Gi-Young Kim Yung Hyun Choi |
| author_facet | Seon Yeong Ji EunJin Bang Hyun Hwangbo Min Yeong Kim Da Hye Kim Young Tae Koo Jin Soo Kim Ki Won Lee Sun Young Park Chan-Young Kwon Hyesook Lee Gi-Young Kim Yung Hyun Choi |
| author_sort | Seon Yeong Ji |
| collection | DOAJ |
| description | Loss of immunity is an important cause in the pathology of infectious disease. This study investigates the effect of <i>Litsea japonica</i> fruit extract (LJFE) as a potential functional food on immunity and hematopoietic function in immunosuppressed BALB/c mice. Immunity-stimulating activity was observed in mice supplemented with LJFE at low (25 mg/kg), medium (50 mg/kg), and high (100 mg/kg) dosage for seven days after administration of cyclophosphamide. LJFE treatment significantly improved spleen injury score (<i>p</i> < 0.001) and body weight (<i>p</i> < 0.02) by approximately two-fold with a high dosage of LJFE (100 mg/kg). Spleen-derived lymphocyte analysis demonstrated that the numbers of clusters of differentiation (CD)4<sup>+</sup> and CD8<sup>+</sup> T-cells were notably increased by approximately two-fold (<i>p</i> < 0.001) with a high dosage of LJFE (100 mg/kg). In mouse splenocytes differentiated into T- and B-lymphocytes, LJFE significantly induced proliferation up to approximately 90% of control for T- (<i>p</i> < 0.001) and B-lymphocytes (<i>p</i> < 0.01) with a high dosage of LJFE (100 mg/kg). Furthermore, LJFE significantly recovered the numbers of white blood cells, red blood cells, and platelets. Enzyme-linked immunosorbent assay revealed that serum levels of immune-related cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-2, and interferon (IFN)-γ, were notably Increased. In addition, serum levels of immunoglobulin (Ig) A, IgM, and IgG were restored by LJFE treatment. This study provides a reference to use <i>L. japonica</i> as a functional food ingredient to improve immunity and hematological function in humans. |
| first_indexed | 2024-03-11T10:09:16Z |
| format | Article |
| id | doaj.art-c4d13559b86441ccac5c219e901b3144 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T10:09:16Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-c4d13559b86441ccac5c219e901b31442023-11-16T14:51:18ZengMDPI AGApplied Sciences2076-34172022-12-0113114510.3390/app13010145Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c MiceSeon Yeong Ji0EunJin Bang1Hyun Hwangbo2Min Yeong Kim3Da Hye Kim4Young Tae Koo5Jin Soo Kim6Ki Won Lee7Sun Young Park8Chan-Young Kwon9Hyesook Lee10Gi-Young Kim11Yung Hyun Choi12Anti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaAnti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaAnti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaAnti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaAnti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaNatural Products Convergence R&D Division, Kwangdong Pharmaceutical Co., Ltd., Seoul 06650, Republic of KoreaNatural Products Convergence R&D Division, Kwangdong Pharmaceutical Co., Ltd., Seoul 06650, Republic of KoreaNatural Products Convergence R&D Division, Kwangdong Pharmaceutical Co., Ltd., Seoul 06650, Republic of KoreaNatural Products Convergence R&D Division, Kwangdong Pharmaceutical Co., Ltd., Seoul 06650, Republic of KoreaDepartment of Oriental Neuropsychiatry, College of Korean Medicine, Dong-eui University, Busan 47227, Republic of KoreaDepartment of Convergence Medicine, Pusan National University School of Medicine, Yangsan 50612, Republic of KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, Republic of KoreaAnti-Aging Research Center, Dong-eui University, Busan 47227, Republic of KoreaLoss of immunity is an important cause in the pathology of infectious disease. This study investigates the effect of <i>Litsea japonica</i> fruit extract (LJFE) as a potential functional food on immunity and hematopoietic function in immunosuppressed BALB/c mice. Immunity-stimulating activity was observed in mice supplemented with LJFE at low (25 mg/kg), medium (50 mg/kg), and high (100 mg/kg) dosage for seven days after administration of cyclophosphamide. LJFE treatment significantly improved spleen injury score (<i>p</i> < 0.001) and body weight (<i>p</i> < 0.02) by approximately two-fold with a high dosage of LJFE (100 mg/kg). Spleen-derived lymphocyte analysis demonstrated that the numbers of clusters of differentiation (CD)4<sup>+</sup> and CD8<sup>+</sup> T-cells were notably increased by approximately two-fold (<i>p</i> < 0.001) with a high dosage of LJFE (100 mg/kg). In mouse splenocytes differentiated into T- and B-lymphocytes, LJFE significantly induced proliferation up to approximately 90% of control for T- (<i>p</i> < 0.001) and B-lymphocytes (<i>p</i> < 0.01) with a high dosage of LJFE (100 mg/kg). Furthermore, LJFE significantly recovered the numbers of white blood cells, red blood cells, and platelets. Enzyme-linked immunosorbent assay revealed that serum levels of immune-related cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-2, and interferon (IFN)-γ, were notably Increased. In addition, serum levels of immunoglobulin (Ig) A, IgM, and IgG were restored by LJFE treatment. This study provides a reference to use <i>L. japonica</i> as a functional food ingredient to improve immunity and hematological function in humans.https://www.mdpi.com/2076-3417/13/1/145<i>Litsea japonica</i>immunityhematopoietic functioncytokinesmice |
| spellingShingle | Seon Yeong Ji EunJin Bang Hyun Hwangbo Min Yeong Kim Da Hye Kim Young Tae Koo Jin Soo Kim Ki Won Lee Sun Young Park Chan-Young Kwon Hyesook Lee Gi-Young Kim Yung Hyun Choi Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice Applied Sciences <i>Litsea japonica</i> immunity hematopoietic function cytokines mice |
| title | Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice |
| title_full | Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice |
| title_fullStr | Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice |
| title_full_unstemmed | Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice |
| title_short | Improvement of Immune and Hematopoietic Functions by <i>Litsea japonica</i> Fruit Extract in Cyclophosphamide-Treated BALB/c Mice |
| title_sort | improvement of immune and hematopoietic functions by i litsea japonica i fruit extract in cyclophosphamide treated balb c mice |
| topic | <i>Litsea japonica</i> immunity hematopoietic function cytokines mice |
| url | https://www.mdpi.com/2076-3417/13/1/145 |
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