Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma
Objective: To determine the Immunohistochemical expression of BRAF V600E and Epidermal growth factor receptor (EGFR) in ameloblastoma and correlate the expression with age and gender of patients, and patterns and types of ameloblastoma. Material & Methods: 39 cases were retrieved with their for...
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2022-03-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/786 |
| _version_ | 1827368719536357376 |
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| author | Namrah Bashir Muhammad Asif Iqraa Shakeel Malik Nighat Araa Farhat Rashid Hafeez Uddin Aieman Bashir Numrah Shakeel Malik |
| author_facet | Namrah Bashir Muhammad Asif Iqraa Shakeel Malik Nighat Araa Farhat Rashid Hafeez Uddin Aieman Bashir Numrah Shakeel Malik |
| author_sort | Namrah Bashir |
| collection | DOAJ |
| description | Objective: To determine the Immunohistochemical expression of BRAF V600E and Epidermal growth factor receptor (EGFR) in ameloblastoma and correlate the expression with age and gender of patients, and patterns and types of ameloblastoma.
Material & Methods: 39 cases were retrieved with their formalin-fixed, paraffin embedded blocks, trimmed and cut into 5 microns sections. They were mounted on slides after staining with routine hematoxylin and eosin followed by Immunohistochemical staining of BRAF V600E and EGFR. Mean and standard deviation were calculated for quantitative variables. Frequency and percentages were calculated for qualitative variables. Chi-square test was employed to assess the significance of difference. The p-value ≤ 0.05 was considered significant.
Results: There were 19 (41.2%) males and 20 (48.7%) female patients. The mean age of patients at which they presented was 39.97 ± 15.505 (mean ± SD) with an age range between 12 to 65 years. 25 (64.1 %) cases showed positive expression of BRAF V600E and 14 (35.8 %) cases showed negative expression of BRAF V600E. 27 (69.2 %) cases showed positive expression of EGFR whereas 6 (15.3 %) cases showed negative expression of EGFR. The p-value was ≤ 0.05 for expression of BRAF V600E with respect to patterns of ameloblastoma and tumor site and expression of EGFR with respect to sub-types of ameloblastoma.
Conclusion: There is positive expression of BRAF V600E (64.1%) and EGFR (74.4%) in different sub-types and patterns of ameloblastoma. Correct assessment with the help of these markers can lead to early diagnosis and use of adjuvant treatment protocols |
| first_indexed | 2024-03-08T09:36:10Z |
| format | Article |
| id | doaj.art-c4d136ff48b24360b446f6bdea9a746b |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T09:36:10Z |
| publishDate | 2022-03-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-c4d136ff48b24360b446f6bdea9a746b2024-01-30T09:15:40ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352022-03-0171293510.31557/apjcb.2022.7.1.29-35786Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in AmeloblastomaNamrah Bashir0Muhammad Asif1Iqraa Shakeel Malik2Nighat Araa3Farhat Rashid4Hafeez Uddin5Aieman Bashir6Numrah Shakeel Malik7Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan.Consultant Histopathologist, , Armed Forces Institute of Pathology, Rawalpindi, PakistanHistopathology department, Armed Forces Institute of Pathology, Rawalpindi, PakistanAssociate Professor, Army Medical College, Rawalpindi, PakistanConsultant Histopathologist, Armed forces institute of Pathology, PakistanConsultant Histopathologist, , Armed Forces Institute of Pathology, Rawalpindi, PakistanDepartment of Oncology, Fauji Foundation, DHA 1 Islamabad, PakistanDepartment of Surgery, Fauji Foundation, DHA 1 Islamabad, PakistanObjective: To determine the Immunohistochemical expression of BRAF V600E and Epidermal growth factor receptor (EGFR) in ameloblastoma and correlate the expression with age and gender of patients, and patterns and types of ameloblastoma. Material & Methods: 39 cases were retrieved with their formalin-fixed, paraffin embedded blocks, trimmed and cut into 5 microns sections. They were mounted on slides after staining with routine hematoxylin and eosin followed by Immunohistochemical staining of BRAF V600E and EGFR. Mean and standard deviation were calculated for quantitative variables. Frequency and percentages were calculated for qualitative variables. Chi-square test was employed to assess the significance of difference. The p-value ≤ 0.05 was considered significant. Results: There were 19 (41.2%) males and 20 (48.7%) female patients. The mean age of patients at which they presented was 39.97 ± 15.505 (mean ± SD) with an age range between 12 to 65 years. 25 (64.1 %) cases showed positive expression of BRAF V600E and 14 (35.8 %) cases showed negative expression of BRAF V600E. 27 (69.2 %) cases showed positive expression of EGFR whereas 6 (15.3 %) cases showed negative expression of EGFR. The p-value was ≤ 0.05 for expression of BRAF V600E with respect to patterns of ameloblastoma and tumor site and expression of EGFR with respect to sub-types of ameloblastoma. Conclusion: There is positive expression of BRAF V600E (64.1%) and EGFR (74.4%) in different sub-types and patterns of ameloblastoma. Correct assessment with the help of these markers can lead to early diagnosis and use of adjuvant treatment protocolshttp://www.waocp.com/journal/index.php/apjcb/article/view/786biomarkers, erbb receptors, immunohistochemistry, maxillofacial pathology, proto-oncogene proteins braf v600e |
| spellingShingle | Namrah Bashir Muhammad Asif Iqraa Shakeel Malik Nighat Araa Farhat Rashid Hafeez Uddin Aieman Bashir Numrah Shakeel Malik Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma Asian Pacific Journal of Cancer Biology biomarkers, erbb receptors, immunohistochemistry, maxillofacial pathology, proto-oncogene proteins braf v600e |
| title | Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma |
| title_full | Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma |
| title_fullStr | Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma |
| title_full_unstemmed | Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma |
| title_short | Frequency of Expression of BRAF V600E and Epidermal Growth Factor Receptor (EGFR) in Ameloblastoma |
| title_sort | frequency of expression of braf v600e and epidermal growth factor receptor egfr in ameloblastoma |
| topic | biomarkers, erbb receptors, immunohistochemistry, maxillofacial pathology, proto-oncogene proteins braf v600e |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/786 |
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