Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation
Genetically modified pigs have been considered favorable resources in xenotransplantation. Microinjection of randomly integrating transgenes into zygotes, somatic cell nuclear transfer, homologous recombination, zinc finger nucleases, transcription activator-like effector nucleases, and most recentl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.01711/full |
| _version_ | 1819139767101554688 |
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| author | Meisam Naeimi Kararoudi Seyyed S. Hejazi Ezgi Elmas Mats Hellström Maryam Naeimi Kararoudi Arvind M. Padma Dean Lee Hamid Dolatshad |
| author_facet | Meisam Naeimi Kararoudi Seyyed S. Hejazi Ezgi Elmas Mats Hellström Maryam Naeimi Kararoudi Arvind M. Padma Dean Lee Hamid Dolatshad |
| author_sort | Meisam Naeimi Kararoudi |
| collection | DOAJ |
| description | Genetically modified pigs have been considered favorable resources in xenotransplantation. Microinjection of randomly integrating transgenes into zygotes, somatic cell nuclear transfer, homologous recombination, zinc finger nucleases, transcription activator-like effector nucleases, and most recently, clustered regularly interspaced short palindromic repeats-cas9 (CRISPR/Cas9) are the techniques that have been used to generate these animals. Here, we provide an overview of the CRISPR approaches that have been used to modify genes which are vital in improving xenograft survival rate, including cytidine monophosphate-N-acetylneuraminic acid hydroxylase, B1,4N-acetylgalactosaminyltransferase, isoglobotrihexosylceramide synthase, class I MHC, von Willebrand factor, C3, and porcine endogenous retroviruses. In addition, we will mention the importance of potential candidate genes which could be targeted using CRISPR/Cas9. |
| first_indexed | 2024-12-22T11:27:53Z |
| format | Article |
| id | doaj.art-c4d41d8673254b4095c89cc24e8693ab |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T11:27:53Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c4d41d8673254b4095c89cc24e8693ab2022-12-21T18:27:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-09-01910.3389/fimmu.2018.01711343935Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in XenotransplantationMeisam Naeimi Kararoudi0Seyyed S. Hejazi1Ezgi Elmas2Mats Hellström3Maryam Naeimi Kararoudi4Arvind M. Padma5Dean Lee6Hamid Dolatshad7The Childhood Cancer Center at Nationwide Children’s Hospital, Columbus, OH, United StatesDepartment of Basic Science of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, IranThe Childhood Cancer Center at Nationwide Children’s Hospital, Columbus, OH, United StatesLaboratory for Transplantation and Regenerative Medicine, Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenEye Research Center, Farabi Eye Hospital, Tehran University of Medical Science, Tehran, IranLaboratory for Transplantation and Regenerative Medicine, Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenThe Childhood Cancer Center at Nationwide Children’s Hospital, Columbus, OH, United StatesBloodwise Molecular Haematology Unit, Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, Oxford, United KingdomGenetically modified pigs have been considered favorable resources in xenotransplantation. Microinjection of randomly integrating transgenes into zygotes, somatic cell nuclear transfer, homologous recombination, zinc finger nucleases, transcription activator-like effector nucleases, and most recently, clustered regularly interspaced short palindromic repeats-cas9 (CRISPR/Cas9) are the techniques that have been used to generate these animals. Here, we provide an overview of the CRISPR approaches that have been used to modify genes which are vital in improving xenograft survival rate, including cytidine monophosphate-N-acetylneuraminic acid hydroxylase, B1,4N-acetylgalactosaminyltransferase, isoglobotrihexosylceramide synthase, class I MHC, von Willebrand factor, C3, and porcine endogenous retroviruses. In addition, we will mention the importance of potential candidate genes which could be targeted using CRISPR/Cas9.https://www.frontiersin.org/article/10.3389/fimmu.2018.01711/fullclustered regularly interspaced short palindromic repeats/Cas9xenotransplantationzinc finger nucleases–transcription activator-like effector nuclease–clustered regularly interspaced short palindromic repeats/Castransplantation immunologygene editing |
| spellingShingle | Meisam Naeimi Kararoudi Seyyed S. Hejazi Ezgi Elmas Mats Hellström Maryam Naeimi Kararoudi Arvind M. Padma Dean Lee Hamid Dolatshad Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation Frontiers in Immunology clustered regularly interspaced short palindromic repeats/Cas9 xenotransplantation zinc finger nucleases–transcription activator-like effector nuclease–clustered regularly interspaced short palindromic repeats/Cas transplantation immunology gene editing |
| title | Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation |
| title_full | Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation |
| title_fullStr | Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation |
| title_full_unstemmed | Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation |
| title_short | Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation |
| title_sort | clustered regularly interspaced short palindromic repeats cas9 gene editing technique in xenotransplantation |
| topic | clustered regularly interspaced short palindromic repeats/Cas9 xenotransplantation zinc finger nucleases–transcription activator-like effector nuclease–clustered regularly interspaced short palindromic repeats/Cas transplantation immunology gene editing |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.01711/full |
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