Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations
Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous del...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/2/281 |
| _version_ | 1797396008740585472 |
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| author | Isaías Sanmartín Luis Sendra Inés Moret María José Herrero Salvador F. Aliño |
| author_facet | Isaías Sanmartín Luis Sendra Inés Moret María José Herrero Salvador F. Aliño |
| author_sort | Isaías Sanmartín |
| collection | DOAJ |
| description | Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. The vector characterization and optimization were carried out using liposomes with entrapped carboxyfluorescein and adapted electrophoretic assays. Two types of lipopolyplexes (containing hydrophilic or lipophilic antigens) were employed to evaluate their interest in vaccination. The lipopolyplex loaded with an extract of water-soluble melanoma proteins proved to efficiently induce humoral response in murine melanoma model, increasing the levels of IgM and IgG. The specificity of the immune response induced by the lipopolyplex was demonstrated in mice with the lipopolyplex containing the GD3 ganglioside lipid antigen, abundant in melanoma cells. The levels of anti-GD3 IgG increased markedly without modifying the expression of humoral antibodies against other gangliosides. |
| first_indexed | 2024-03-09T00:44:05Z |
| format | Article |
| id | doaj.art-c4d6427a3c0048b88e8b461fcb4c5709 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T00:44:05Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-c4d6427a3c0048b88e8b461fcb4c57092023-12-11T17:41:01ZengMDPI AGPharmaceutics1999-49232021-02-0113228110.3390/pharmaceutics13020281Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine FormulationsIsaías Sanmartín0Luis Sendra1Inés Moret2María José Herrero3Salvador F. Aliño4Faculty of Veterinary and Experimental Sciences, Universidad Católica de Valencia, 46001 Valencia, SpainPharmacology Department, Faculty of Medicine, Universidad de Valencia, 46010 Valencia, SpainPharmacology Department, Faculty of Medicine, Universidad de Valencia, 46010 Valencia, SpainPharmacology Department, Faculty of Medicine, Universidad de Valencia, 46010 Valencia, SpainPharmacology Department, Faculty of Medicine, Universidad de Valencia, 46010 Valencia, SpainVector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. The vector characterization and optimization were carried out using liposomes with entrapped carboxyfluorescein and adapted electrophoretic assays. Two types of lipopolyplexes (containing hydrophilic or lipophilic antigens) were employed to evaluate their interest in vaccination. The lipopolyplex loaded with an extract of water-soluble melanoma proteins proved to efficiently induce humoral response in murine melanoma model, increasing the levels of IgM and IgG. The specificity of the immune response induced by the lipopolyplex was demonstrated in mice with the lipopolyplex containing the GD3 ganglioside lipid antigen, abundant in melanoma cells. The levels of anti-GD3 IgG increased markedly without modifying the expression of humoral antibodies against other gangliosides.https://www.mdpi.com/1999-4923/13/2/281lipopolyplexesmulticompartmental lipopolyplexesmelanomanon-viral gene transferantitumor immunizationvaccine |
| spellingShingle | Isaías Sanmartín Luis Sendra Inés Moret María José Herrero Salvador F. Aliño Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations Pharmaceutics lipopolyplexes multicompartmental lipopolyplexes melanoma non-viral gene transfer antitumor immunization vaccine |
| title | Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations |
| title_full | Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations |
| title_fullStr | Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations |
| title_full_unstemmed | Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations |
| title_short | Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations |
| title_sort | multicompartmental lipopolyplex as vehicle for antigens and genes delivery in vaccine formulations |
| topic | lipopolyplexes multicompartmental lipopolyplexes melanoma non-viral gene transfer antitumor immunization vaccine |
| url | https://www.mdpi.com/1999-4923/13/2/281 |
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