Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China
<i>Streptococcus suis</i> is an important zoonotic pathogen. Serotype 2 and sequence type (ST) 1 are the most frequently reported strains in both infected humans and pigs. ST7 is only endemic to China, and it was responsible for outbreaks in 1998 and 2005 in China. In the present study,...
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MDPI AG
2019-10-01
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| Online Access: | https://www.mdpi.com/2076-0817/8/4/187 |
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| author | Mingliu Wang Pengcheng Du Jianping Wang Ruiting Lan Jun Huang Ming Luo Yan Jiang Jun Zeng Yi Quan Zhaohui Shi Han Zheng |
| author_facet | Mingliu Wang Pengcheng Du Jianping Wang Ruiting Lan Jun Huang Ming Luo Yan Jiang Jun Zeng Yi Quan Zhaohui Shi Han Zheng |
| author_sort | Mingliu Wang |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> is an important zoonotic pathogen. Serotype 2 and sequence type (ST) 1 are the most frequently reported strains in both infected humans and pigs. ST7 is only endemic to China, and it was responsible for outbreaks in 1998 and 2005 in China. In the present study, 38 sporadic ST7 <i>S. suis</i> strains, which mostly caused sepsis, were collected from patients in the Guangxi Zhuang Autonomous Region (GX) between 2007 and 2018. Of 38 sporadic ST7 strains, serotype 14 was the most frequent (27 strains, 71.1%), followed by serotype 2 (11 strains, 28.9%). The phylogenetic structure of the ST7 population, including epidemic and sporadic ST7 strains, was constructed using mutational single-nucleotide polymorphisms (SNPs). High diversity within the ST7 population was revealed and divided into five lineages. Only one sporadic ST7 strain, GX14, from a <i>Streptococcal</i> toxic-shock-like syndrome (STSLS) patient was clustered into the same lineage as the epidemic strains. GX14 and the epidemic strains diverged in 1974. The sporadic ST7 strains of GX were mainly clustered into lineage 5, which emerged in 1980. Comparing to genome of epidemic strain, the major differences in genome of sporadic ST7 strains of GX was the absence of 89 kb pathogenicity island (PAI) specific to epidemic strain and insertion of 128 kb ICE_phage tandem MGE or ICE portion of the MGE. These mobile elements play a significant role in the horizontal transfer of antibiotic resistance genes in sporadic ST7 strains. Our results enhanced the understanding of the evolution of the ST7 strains and their ability to cause life-threatening infections in humans. |
| first_indexed | 2024-04-11T22:24:02Z |
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| spelling | doaj.art-c4d6a1817eb6415cb4e6b105cca5ee4f2022-12-22T03:59:54ZengMDPI AGPathogens2076-08172019-10-018418710.3390/pathogens8040187pathogens8040187Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of ChinaMingliu Wang0Pengcheng Du1Jianping Wang2Ruiting Lan3Jun Huang4Ming Luo5Yan Jiang6Jun Zeng7Yi Quan8Zhaohui Shi9Han Zheng10Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning 530028, ChinaBeijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, ChinaSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, AustraliaGuangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning 530028, ChinaYulin Center for Disease Prevention and Control, Yulin 537000, ChinaQinzhou Center for Disease Prevention and Control, Qinzhou 535099, ChinaGuangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning 530028, ChinaGuangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning 530028, ChinaGuilin Center for Disease Prevention and Control, Guilin 541001, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China<i>Streptococcus suis</i> is an important zoonotic pathogen. Serotype 2 and sequence type (ST) 1 are the most frequently reported strains in both infected humans and pigs. ST7 is only endemic to China, and it was responsible for outbreaks in 1998 and 2005 in China. In the present study, 38 sporadic ST7 <i>S. suis</i> strains, which mostly caused sepsis, were collected from patients in the Guangxi Zhuang Autonomous Region (GX) between 2007 and 2018. Of 38 sporadic ST7 strains, serotype 14 was the most frequent (27 strains, 71.1%), followed by serotype 2 (11 strains, 28.9%). The phylogenetic structure of the ST7 population, including epidemic and sporadic ST7 strains, was constructed using mutational single-nucleotide polymorphisms (SNPs). High diversity within the ST7 population was revealed and divided into five lineages. Only one sporadic ST7 strain, GX14, from a <i>Streptococcal</i> toxic-shock-like syndrome (STSLS) patient was clustered into the same lineage as the epidemic strains. GX14 and the epidemic strains diverged in 1974. The sporadic ST7 strains of GX were mainly clustered into lineage 5, which emerged in 1980. Comparing to genome of epidemic strain, the major differences in genome of sporadic ST7 strains of GX was the absence of 89 kb pathogenicity island (PAI) specific to epidemic strain and insertion of 128 kb ICE_phage tandem MGE or ICE portion of the MGE. These mobile elements play a significant role in the horizontal transfer of antibiotic resistance genes in sporadic ST7 strains. Our results enhanced the understanding of the evolution of the ST7 strains and their ability to cause life-threatening infections in humans.https://www.mdpi.com/2076-0817/8/4/187<i>streptococcus suis</i>sequence type 7sporadic strainserotype 14phylogenetic structurecomparative genomes |
| spellingShingle | Mingliu Wang Pengcheng Du Jianping Wang Ruiting Lan Jun Huang Ming Luo Yan Jiang Jun Zeng Yi Quan Zhaohui Shi Han Zheng Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China Pathogens <i>streptococcus suis</i> sequence type 7 sporadic strain serotype 14 phylogenetic structure comparative genomes |
| title | Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China |
| title_full | Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China |
| title_fullStr | Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China |
| title_full_unstemmed | Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China |
| title_short | Genomic Epidemiology of <i>Streptococcus suis</i> Sequence Type 7 Sporadic Infections in the Guangxi Zhuang Autonomous Region of China |
| title_sort | genomic epidemiology of i streptococcus suis i sequence type 7 sporadic infections in the guangxi zhuang autonomous region of china |
| topic | <i>streptococcus suis</i> sequence type 7 sporadic strain serotype 14 phylogenetic structure comparative genomes |
| url | https://www.mdpi.com/2076-0817/8/4/187 |
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