In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i>
<i>Agathis</i> species are widely distributed around Southeast Asia, Australasia, South Pacific islands, and etc. Traditionally, <i>Agathis</i> species have been used as the folk medicines, the common ethnopharmacological uses of <i>Agathis</i> genus are the treat...
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MDPI AG
2023-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/9/2269 |
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| author | Yu-Tung Ho I-Hsuan Liu Shang-Tzen Chang Sheng-Yang Wang Hui-Ting Chang |
| author_facet | Yu-Tung Ho I-Hsuan Liu Shang-Tzen Chang Sheng-Yang Wang Hui-Ting Chang |
| author_sort | Yu-Tung Ho |
| collection | DOAJ |
| description | <i>Agathis</i> species are widely distributed around Southeast Asia, Australasia, South Pacific islands, and etc. Traditionally, <i>Agathis</i> species have been used as the folk medicines, the common ethnopharmacological uses of <i>Agathis</i> genus are the treatments of headache and myalgia. This study aims to investigate the chemical composition of <i>Agathis dammara</i> (Lamb.) Rich. leaf essential oil and to explore its antimelanogenesis effect. The chemical constituents of leaf essential oil are analyzed using gas chromatography-mass spectrometry (GC-MS), the major constituents of leaf essential oil are sesquiterpenoids. The major constituents are δ-cadinene (16.12%), followed by γ-gurjunene (15.57%), 16-kaurene (12.43%), β-caryophyllene (8.58%), germacrene D (8.53%), and γ-cadinene (5.33%). As for the in vitro antityrosinase activity, leaf essential oil inhibit the tyrosinase activity of mushroom when the substrate is 3,4-dihydroxyphenylalanine (L-DOPA). Leaf essential oil prevents tyrosinase from acting as diphenolase and catalyzing L-DOPA to dopaquinone, and converting into dark melanin pigments. <i>A. dammara</i> leaf essential oil also exhibits the in vivo antimelanogenesis effect, leaf essential oil reduces 43.48% of melanin formation in zebrafish embryos at the concentration of 50 μg/mL. Results reveal <i>A. dammara</i> leaf essential oil has the potential for developing the skin whitening drug and depigmentation ingredient for hyperpigmentary disorders. |
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| format | Article |
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| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T22:15:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-c4d8c3b1ef21472788a48baed46485bd2023-11-19T12:27:22ZengMDPI AGPharmaceutics1999-49232023-09-01159226910.3390/pharmaceutics15092269In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i>Yu-Tung Ho0I-Hsuan Liu1Shang-Tzen Chang2Sheng-Yang Wang3Hui-Ting Chang4School of Forestry and Resource Conservation, National Taiwan University, Taipei 10617, TaiwanDepartment of Animal Science and Technology, National Taiwan University, Taipei 10617, TaiwanSchool of Forestry and Resource Conservation, National Taiwan University, Taipei 10617, TaiwanDepartment of Forestry, National Chung Hsing University, Taichung 40227, TaiwanSchool of Forestry and Resource Conservation, National Taiwan University, Taipei 10617, Taiwan<i>Agathis</i> species are widely distributed around Southeast Asia, Australasia, South Pacific islands, and etc. Traditionally, <i>Agathis</i> species have been used as the folk medicines, the common ethnopharmacological uses of <i>Agathis</i> genus are the treatments of headache and myalgia. This study aims to investigate the chemical composition of <i>Agathis dammara</i> (Lamb.) Rich. leaf essential oil and to explore its antimelanogenesis effect. The chemical constituents of leaf essential oil are analyzed using gas chromatography-mass spectrometry (GC-MS), the major constituents of leaf essential oil are sesquiterpenoids. The major constituents are δ-cadinene (16.12%), followed by γ-gurjunene (15.57%), 16-kaurene (12.43%), β-caryophyllene (8.58%), germacrene D (8.53%), and γ-cadinene (5.33%). As for the in vitro antityrosinase activity, leaf essential oil inhibit the tyrosinase activity of mushroom when the substrate is 3,4-dihydroxyphenylalanine (L-DOPA). Leaf essential oil prevents tyrosinase from acting as diphenolase and catalyzing L-DOPA to dopaquinone, and converting into dark melanin pigments. <i>A. dammara</i> leaf essential oil also exhibits the in vivo antimelanogenesis effect, leaf essential oil reduces 43.48% of melanin formation in zebrafish embryos at the concentration of 50 μg/mL. Results reveal <i>A. dammara</i> leaf essential oil has the potential for developing the skin whitening drug and depigmentation ingredient for hyperpigmentary disorders.https://www.mdpi.com/1999-4923/15/9/2269<i>Agathis dammara</i>antimelanogenesis effectantityrosinase activityessential oilmelaninzebrafish (<i>Danio rerio</i>) |
| spellingShingle | Yu-Tung Ho I-Hsuan Liu Shang-Tzen Chang Sheng-Yang Wang Hui-Ting Chang In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> Pharmaceutics <i>Agathis dammara</i> antimelanogenesis effect antityrosinase activity essential oil melanin zebrafish (<i>Danio rerio</i>) |
| title | In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> |
| title_full | In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> |
| title_fullStr | In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> |
| title_full_unstemmed | In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> |
| title_short | In Vitro and In Vivo Antimelanogenesis Effects of Leaf Essential Oil from <i>Agathis dammara</i> |
| title_sort | in vitro and in vivo antimelanogenesis effects of leaf essential oil from i agathis dammara i |
| topic | <i>Agathis dammara</i> antimelanogenesis effect antityrosinase activity essential oil melanin zebrafish (<i>Danio rerio</i>) |
| url | https://www.mdpi.com/1999-4923/15/9/2269 |
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