Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep
Previous studies showed that the NLR family pyrin domain-containing 5 (NLRP5) and NLRP9 genes are two important reproductive genes; however, their effects on sheep litter size are unknown. Therefore, in this study, we first genotyped seven sheep breeds via the MassARRAY<sup>®</sup> SNP s...
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MDPI AG
2020-04-01
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| author | Zhuangbiao Zhang Jishun Tang Xiaoyun He Ran Di Mingxing Chu |
| author_facet | Zhuangbiao Zhang Jishun Tang Xiaoyun He Ran Di Mingxing Chu |
| author_sort | Zhuangbiao Zhang |
| collection | DOAJ |
| description | Previous studies showed that the NLR family pyrin domain-containing 5 (NLRP5) and NLRP9 genes are two important reproductive genes; however, their effects on sheep litter size are unknown. Therefore, in this study, we first genotyped seven sheep breeds via the MassARRAY<sup>®</sup> SNP system at the loci g.60495375A > G, g.60495363G > A, and g.60499690C > A in <i>NLRP5</i>, and g.59030623T > C and g.59043397A > C in <i>NLRP9</i>. Our results revealed that each locus in most sheep breeds contained three genotypes. Then, we conducted population genetic analysis of single nucleotide polymorphisms in <i>NLRP5</i> and <i>NLRP9</i>, and we found that the polymorphism information content value in all sheep breeds ranged from 0 to 0.36, and most sheep breeds were under Hardy–Weinberg equilibrium (<i>p</i> > 0.05). Furthermore, association analysis in Small Tail Han sheep indicated that two loci, g.60495363G > A in <i>NLRP5</i> and g.59030623T > C in <i>NLRP9</i>, were highly associated with litter size. The mutation in g.60495363G > A may decrease interactions of NLRP5 with proteins, such as GDF9, whereas the mutation in g.59030623T > C may enhance the combining capacity of NLRP9 with these proteins; consequently, these mutations may influence the ovulation rate and even litter size. The findings of our study provide valuable genetic markers that can be used to improve the breeding of sheep and even other mammals. |
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| last_indexed | 2024-03-10T20:27:28Z |
| publishDate | 2020-04-01 |
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| spelling | doaj.art-c4dad090f9214cbbbdd586459d92a6bd2023-11-19T21:44:08ZengMDPI AGAnimals2076-26152020-04-0110468910.3390/ani10040689Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han SheepZhuangbiao Zhang0Jishun Tang1Xiaoyun He2Ran Di3Mingxing Chu4Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaPrevious studies showed that the NLR family pyrin domain-containing 5 (NLRP5) and NLRP9 genes are two important reproductive genes; however, their effects on sheep litter size are unknown. Therefore, in this study, we first genotyped seven sheep breeds via the MassARRAY<sup>®</sup> SNP system at the loci g.60495375A > G, g.60495363G > A, and g.60499690C > A in <i>NLRP5</i>, and g.59030623T > C and g.59043397A > C in <i>NLRP9</i>. Our results revealed that each locus in most sheep breeds contained three genotypes. Then, we conducted population genetic analysis of single nucleotide polymorphisms in <i>NLRP5</i> and <i>NLRP9</i>, and we found that the polymorphism information content value in all sheep breeds ranged from 0 to 0.36, and most sheep breeds were under Hardy–Weinberg equilibrium (<i>p</i> > 0.05). Furthermore, association analysis in Small Tail Han sheep indicated that two loci, g.60495363G > A in <i>NLRP5</i> and g.59030623T > C in <i>NLRP9</i>, were highly associated with litter size. The mutation in g.60495363G > A may decrease interactions of NLRP5 with proteins, such as GDF9, whereas the mutation in g.59030623T > C may enhance the combining capacity of NLRP9 with these proteins; consequently, these mutations may influence the ovulation rate and even litter size. The findings of our study provide valuable genetic markers that can be used to improve the breeding of sheep and even other mammals.https://www.mdpi.com/2076-2615/10/4/689<i>NLRP5</i><i>NLRP9</i>SNPssheeplitter sizeGDF9 |
| spellingShingle | Zhuangbiao Zhang Jishun Tang Xiaoyun He Ran Di Mingxing Chu Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep Animals <i>NLRP5</i> <i>NLRP9</i> SNPs sheep litter size GDF9 |
| title | Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep |
| title_full | Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep |
| title_fullStr | Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep |
| title_full_unstemmed | Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep |
| title_short | Mutations in <i>NLRP5</i> and <i>NLRP9</i> Are Associated with Litter Size in Small Tail Han Sheep |
| title_sort | mutations in i nlrp5 i and i nlrp9 i are associated with litter size in small tail han sheep |
| topic | <i>NLRP5</i> <i>NLRP9</i> SNPs sheep litter size GDF9 |
| url | https://www.mdpi.com/2076-2615/10/4/689 |
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