Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage
Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) was used to soak to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five genotypes of Chinese cabbage gen...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01780/full |
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| author | yin lu shuangyan dai aixia gu mengyang liu yanhua wang shuangxia luo yujing zhao shan wang shuxin xuan xueping chen xiaofeng li Guusje Bonnema Jianjun Zhao shuxing shen |
| author_facet | yin lu shuangyan dai aixia gu mengyang liu yanhua wang shuangxia luo yujing zhao shan wang shuxin xuan xueping chen xiaofeng li Guusje Bonnema Jianjun Zhao shuxing shen |
| author_sort | yin lu |
| collection | DOAJ |
| description | Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) was used to soak to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five genotypes of Chinese cabbage genotypes. Mutations in four color-related genes were identified using hHigh resolution melting (HRM) curves of their PCR products. was used to identify mutations in four color-related genes. The results demonstrated that an EMS concentration greater than 0.1% was lethal to microspores. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores.. These conditions also facilitated culturing of microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids plantlets. Our results suggest demonstrate that microspore derived Doubled Haploids from soaking of buds with EMS soaked buds is anrepresents an efficient approach to the rapidly generation generate of Chinese cabbage mutants. |
| first_indexed | 2024-12-21T17:45:07Z |
| format | Article |
| id | doaj.art-c4db37c29a5e48d1915879d75da36747 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T17:45:07Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c4db37c29a5e48d1915879d75da367472022-12-21T18:55:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-11-01710.3389/fpls.2016.01780224907Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbageyin lu0shuangyan dai1aixia gu2mengyang liu3yanhua wang4shuangxia luo5yujing zhao6shan wang7shuxin xuan8xueping chen9xiaofeng li10Guusje Bonnema11Jianjun Zhao12shuxing shen13hebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityhebei agricultural universityWageningen Universityhebei agricultural universityhebei agricultural universityChinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) was used to soak to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five genotypes of Chinese cabbage genotypes. Mutations in four color-related genes were identified using hHigh resolution melting (HRM) curves of their PCR products. was used to identify mutations in four color-related genes. The results demonstrated that an EMS concentration greater than 0.1% was lethal to microspores. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores.. These conditions also facilitated culturing of microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids plantlets. Our results suggest demonstrate that microspore derived Doubled Haploids from soaking of buds with EMS soaked buds is anrepresents an efficient approach to the rapidly generation generate of Chinese cabbage mutants.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01780/fullBrassica rapaMutationEMS mutagenesismicrospore cultureChinese cabbage |
| spellingShingle | yin lu shuangyan dai aixia gu mengyang liu yanhua wang shuangxia luo yujing zhao shan wang shuxin xuan xueping chen xiaofeng li Guusje Bonnema Jianjun Zhao shuxing shen Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage Frontiers in Plant Science Brassica rapa Mutation EMS mutagenesis microspore culture Chinese cabbage |
| title | Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage |
| title_full | Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage |
| title_fullStr | Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage |
| title_full_unstemmed | Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage |
| title_short | Microspore induced Doubled Haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an eEfficient strategy for mutagenesis in Chinese cabbage |
| title_sort | microspore induced doubled haploids production from ethyl methanesulfonate ems soaked flower buds is an eefficient strategy for mutagenesis in chinese cabbage |
| topic | Brassica rapa Mutation EMS mutagenesis microspore culture Chinese cabbage |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01780/full |
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