Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food
Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with dig...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2009-06-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0092_nucleic-acid-lateral-flow-immunoassay-for-the-detection-of-pathogenic-bacteria-from-food.php |
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| author | M. Blažková M. Koets J. H. Wichers A. van Amerongen L. Fukal P. Rauch |
| author_facet | M. Blažková M. Koets J. H. Wichers A. van Amerongen L. Fukal P. Rauch |
| author_sort | M. Blažková |
| collection | DOAJ |
| description | Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal pathogen responsible for the serious illness listeriosis. Conventional microbiological methods for L. monocytogenes detection are laborious and take several days to achieve a confirmed identification. |
| first_indexed | 2024-04-10T08:35:14Z |
| format | Article |
| id | doaj.art-c4db9645a91748e9a10eae8173ff720d |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:14Z |
| publishDate | 2009-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-c4db9645a91748e9a10eae8173ff720d2023-02-23T03:27:32ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-06-0127Special Issue 1S350S35310.17221/959-CJFScjf-200910-0092Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from FoodM. BlažkováM. Koets0J. H. Wichers1A. van Amerongen2L. Fukal3P. Rauch4Department of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 66 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 66 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 66 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 66 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, 66 28 Prague, Czech RepublicNucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal pathogen responsible for the serious illness listeriosis. Conventional microbiological methods for L. monocytogenes detection are laborious and take several days to achieve a confirmed identification.https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0092_nucleic-acid-lateral-flow-immunoassay-for-the-detection-of-pathogenic-bacteria-from-food.phpl. monocytogenesnucleic acid lateral flow immunoassaydna |
| spellingShingle | M. Blažková M. Koets J. H. Wichers A. van Amerongen L. Fukal P. Rauch Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food Czech Journal of Food Sciences l. monocytogenes nucleic acid lateral flow immunoassay dna |
| title | Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food |
| title_full | Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food |
| title_fullStr | Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food |
| title_full_unstemmed | Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food |
| title_short | Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food |
| title_sort | nucleic acid lateral flow immunoassay for the detection of pathogenic bacteria from food |
| topic | l. monocytogenes nucleic acid lateral flow immunoassay dna |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0092_nucleic-acid-lateral-flow-immunoassay-for-the-detection-of-pathogenic-bacteria-from-food.php |
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