<i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution
Global warming poses new challenges for insects to adapt to higher temperatures. Trehalose is the main blood sugar in insects and plays an important role in energy metabolism and stress resistance. The transmembrane transport of trehalose mainly depends on the trehalose transporter (TRET1). <i>...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9019 |
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| author | Huiling Zhou Gaoke Lei Yanting Chen Minsheng You Shijun You |
| author_facet | Huiling Zhou Gaoke Lei Yanting Chen Minsheng You Shijun You |
| author_sort | Huiling Zhou |
| collection | DOAJ |
| description | Global warming poses new challenges for insects to adapt to higher temperatures. Trehalose is the main blood sugar in insects and plays an important role in energy metabolism and stress resistance. The transmembrane transport of trehalose mainly depends on the trehalose transporter (TRET1). <i>Plutella xylostella</i> (L.) is a worldwide agricultural pest; however, the effects of the trehalose transport mechanism and trehalose distribution in tissues on the development, reproduction and temperature adaptation of <i>P. xylostella</i> have yet to be reported. In this study, <i>PxTret1-like</i> was cloned and analyzed regarding its expression pattern. It was found that the expression of <i>PxTret1-like</i> was affected by ambient temperature. The knockout mutation of <i>PxTret1-like</i> was generated using a CRISPR/Cas9 system by targeted knockout. The trehalose content and trehalase activity of mutant <i>P. xylostella</i> increased at different developmental stages. The trehalose content increased in the fat body of the fourth-instar <i>P. xylostella</i>, and decreased in the hemolymph, and there was no significant change in glucose in the fat body and hemolymph. Mutant strains of <i>P. xylostella</i> showed a significantly reduced survival rate, fecundity and ability to withstand extreme temperatures. The results showed that <i>PxTret1-like</i> could affect the development, reproduction and temperature adaptability of <i>P. xylostella</i> by regulating the trehalose content in the fat body and hemolymph. |
| first_indexed | 2024-03-09T09:56:40Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:56:40Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4e6259228c0473b909de8285d0541102023-12-01T23:47:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316901910.3390/ijms23169019<i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue DistributionHuiling Zhou0Gaoke Lei1Yanting Chen2Minsheng You3Shijun You4State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaGlobal warming poses new challenges for insects to adapt to higher temperatures. Trehalose is the main blood sugar in insects and plays an important role in energy metabolism and stress resistance. The transmembrane transport of trehalose mainly depends on the trehalose transporter (TRET1). <i>Plutella xylostella</i> (L.) is a worldwide agricultural pest; however, the effects of the trehalose transport mechanism and trehalose distribution in tissues on the development, reproduction and temperature adaptation of <i>P. xylostella</i> have yet to be reported. In this study, <i>PxTret1-like</i> was cloned and analyzed regarding its expression pattern. It was found that the expression of <i>PxTret1-like</i> was affected by ambient temperature. The knockout mutation of <i>PxTret1-like</i> was generated using a CRISPR/Cas9 system by targeted knockout. The trehalose content and trehalase activity of mutant <i>P. xylostella</i> increased at different developmental stages. The trehalose content increased in the fat body of the fourth-instar <i>P. xylostella</i>, and decreased in the hemolymph, and there was no significant change in glucose in the fat body and hemolymph. Mutant strains of <i>P. xylostella</i> showed a significantly reduced survival rate, fecundity and ability to withstand extreme temperatures. The results showed that <i>PxTret1-like</i> could affect the development, reproduction and temperature adaptability of <i>P. xylostella</i> by regulating the trehalose content in the fat body and hemolymph.https://www.mdpi.com/1422-0067/23/16/9019<i>Plutella xylostella</i>CRISPR/Cas9mutant straintrehalosetemperature adaptability |
| spellingShingle | Huiling Zhou Gaoke Lei Yanting Chen Minsheng You Shijun You <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution International Journal of Molecular Sciences <i>Plutella xylostella</i> CRISPR/Cas9 mutant strain trehalose temperature adaptability |
| title | <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution |
| title_full | <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution |
| title_fullStr | <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution |
| title_full_unstemmed | <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution |
| title_short | <i>PxTret1-like</i> Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution |
| title_sort | i pxtret1 like i affects the temperature adaptability of a cosmopolitan pest by altering trehalose tissue distribution |
| topic | <i>Plutella xylostella</i> CRISPR/Cas9 mutant strain trehalose temperature adaptability |
| url | https://www.mdpi.com/1422-0067/23/16/9019 |
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