Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes
Sucrose synthase (SUS) and sucrose phosphate synthase (SPS) are essential in plant sucrose metabolism. The potato is an important crop worldwide, but systematic analyses of the <i>StSUS</i> and <i>StSPS</i> gene families in potatoes are still lacking. Ten sucrose metabolism-r...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/14/1/70 |
| _version_ | 1797339641579307008 |
|---|---|
| author | Jun Hu Yanfeng Duan Jinxue Hu Shuqing Zhang Guangcun Li |
| author_facet | Jun Hu Yanfeng Duan Jinxue Hu Shuqing Zhang Guangcun Li |
| author_sort | Jun Hu |
| collection | DOAJ |
| description | Sucrose synthase (SUS) and sucrose phosphate synthase (SPS) are essential in plant sucrose metabolism. The potato is an important crop worldwide, but systematic analyses of the <i>StSUS</i> and <i>StSPS</i> gene families in potatoes are still lacking. Ten sucrose metabolism-related genes were identified in this study. The <i>SUSs</i> and <i>SPSs</i> could each be split into three subgroups through phylogenetic analysis. <i>StSUSIc</i> was the most highly expressed gene in different developmental tissues. Ka/Ks analysis showed that <i>StSUSIb</i> and <i>StSUSIc</i> were subjected to more-significant homozygous selection pressure. Our cis-acting element analysis of the <i>StSUS</i> and <i>StSPS</i> promoter sequences showed four elements: defense- and stress-responsive, hormone-responsive, light-responsive, and transcription factor elements. The expression of <i>StSUS</i> and <i>StSPS</i> genes was found to be regulated by circadian rhythm. In the treatments of 1% to 5% sucrose, glucose, and fructose, the expression of <i>StSUS</i> and <i>StSPS</i> family genes was enhanced by sucrose, but inhibited at high-glucose and fructose concentrations. This study identified six <i>StSUS</i> and four <i>StSPS</i> genes and analyzed their gene structure, conserved motifs, chromosome position, promoter elements, phylogenetic tree, and tissue-specific expression patterns. Our results will motivate more research into the biological process underlying the genes of sucrose metabolism in potatoes. |
| first_indexed | 2024-03-08T09:51:17Z |
| format | Article |
| id | doaj.art-c4e79ec0bdeb4a4d9f446865721db5da |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-08T09:51:17Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-c4e79ec0bdeb4a4d9f446865721db5da2024-01-29T14:05:14ZengMDPI AGMetabolites2218-19892024-01-011417010.3390/metabo14010070Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in PotatoesJun Hu0Yanfeng Duan1Jinxue Hu2Shuqing Zhang3Guangcun Li4State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaShijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, ChinaShijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSucrose synthase (SUS) and sucrose phosphate synthase (SPS) are essential in plant sucrose metabolism. The potato is an important crop worldwide, but systematic analyses of the <i>StSUS</i> and <i>StSPS</i> gene families in potatoes are still lacking. Ten sucrose metabolism-related genes were identified in this study. The <i>SUSs</i> and <i>SPSs</i> could each be split into three subgroups through phylogenetic analysis. <i>StSUSIc</i> was the most highly expressed gene in different developmental tissues. Ka/Ks analysis showed that <i>StSUSIb</i> and <i>StSUSIc</i> were subjected to more-significant homozygous selection pressure. Our cis-acting element analysis of the <i>StSUS</i> and <i>StSPS</i> promoter sequences showed four elements: defense- and stress-responsive, hormone-responsive, light-responsive, and transcription factor elements. The expression of <i>StSUS</i> and <i>StSPS</i> genes was found to be regulated by circadian rhythm. In the treatments of 1% to 5% sucrose, glucose, and fructose, the expression of <i>StSUS</i> and <i>StSPS</i> family genes was enhanced by sucrose, but inhibited at high-glucose and fructose concentrations. This study identified six <i>StSUS</i> and four <i>StSPS</i> genes and analyzed their gene structure, conserved motifs, chromosome position, promoter elements, phylogenetic tree, and tissue-specific expression patterns. Our results will motivate more research into the biological process underlying the genes of sucrose metabolism in potatoes.https://www.mdpi.com/2218-1989/14/1/70<i>Solanum tuberosum</i> L.sucrose metabolismgene expressionbioinformatics analysis |
| spellingShingle | Jun Hu Yanfeng Duan Jinxue Hu Shuqing Zhang Guangcun Li Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes Metabolites <i>Solanum tuberosum</i> L. sucrose metabolism gene expression bioinformatics analysis |
| title | Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes |
| title_full | Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes |
| title_fullStr | Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes |
| title_full_unstemmed | Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes |
| title_short | Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes |
| title_sort | phylogenetic and expression analysis of the sucrose synthase and sucrose phosphate synthase gene family in potatoes |
| topic | <i>Solanum tuberosum</i> L. sucrose metabolism gene expression bioinformatics analysis |
| url | https://www.mdpi.com/2218-1989/14/1/70 |
| work_keys_str_mv | AT junhu phylogeneticandexpressionanalysisofthesucrosesynthaseandsucrosephosphatesynthasegenefamilyinpotatoes AT yanfengduan phylogeneticandexpressionanalysisofthesucrosesynthaseandsucrosephosphatesynthasegenefamilyinpotatoes AT jinxuehu phylogeneticandexpressionanalysisofthesucrosesynthaseandsucrosephosphatesynthasegenefamilyinpotatoes AT shuqingzhang phylogeneticandexpressionanalysisofthesucrosesynthaseandsucrosephosphatesynthasegenefamilyinpotatoes AT guangcunli phylogeneticandexpressionanalysisofthesucrosesynthaseandsucrosephosphatesynthasegenefamilyinpotatoes |