Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development
The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and foun...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-05-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/25932 |
| _version_ | 1811199393218428928 |
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| author | Max van Lessen Shannon Shibata-Germanos Andreas van Impel Thomas A Hawkins Jason Rihel Stefan Schulte-Merker |
| author_facet | Max van Lessen Shannon Shibata-Germanos Andreas van Impel Thomas A Hawkins Jason Rihel Stefan Schulte-Merker |
| author_sort | Max van Lessen |
| collection | DOAJ |
| description | The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc. BLECs endocytose macromolecules in a selective manner, which can be blocked by injection of mannose receptor ligands. This first report on brain lymphatic endothelial cells in a vertebrate embryo identifies cells with unique features, including the uptake of macromolecules at a single cell level. Future studies will address whether this represents an uptake mechanism that is conserved in mammals and how these cells affect functions of the embryonic and adult brain. |
| first_indexed | 2024-04-12T01:47:35Z |
| format | Article |
| id | doaj.art-c4e7fc95512841e39cabefef9b191d7b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:47:35Z |
| publishDate | 2017-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c4e7fc95512841e39cabefef9b191d7b2022-12-22T03:53:02ZengeLife Sciences Publications LtdeLife2050-084X2017-05-01610.7554/eLife.25932Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic developmentMax van Lessen0Shannon Shibata-Germanos1https://orcid.org/0000-0002-4250-7497Andreas van Impel2https://orcid.org/0000-0002-4737-3547Thomas A Hawkins3Jason Rihel4https://orcid.org/0000-0003-4067-2066Stefan Schulte-Merker5https://orcid.org/0000-0003-3617-8807Institute of Cardiovascular Organogenesis and Regeneration, WWU Münster, Münster, Germany; Faculty of Medicine, WWU Münster, Münster, Germany; Cells-in-Motion Cluster of Excellence, WWU Münster, Münster, GermanyDepartment of Cell and Developmental Biology, University College London, London, United KingdomInstitute of Cardiovascular Organogenesis and Regeneration, WWU Münster, Münster, Germany; Faculty of Medicine, WWU Münster, Münster, Germany; Cells-in-Motion Cluster of Excellence, WWU Münster, Münster, GermanyDepartment of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Cell and Developmental Biology, University College London, London, United KingdomInstitute of Cardiovascular Organogenesis and Regeneration, WWU Münster, Münster, Germany; Faculty of Medicine, WWU Münster, Münster, Germany; Cells-in-Motion Cluster of Excellence, WWU Münster, Münster, GermanyThe lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc. BLECs endocytose macromolecules in a selective manner, which can be blocked by injection of mannose receptor ligands. This first report on brain lymphatic endothelial cells in a vertebrate embryo identifies cells with unique features, including the uptake of macromolecules at a single cell level. Future studies will address whether this represents an uptake mechanism that is conserved in mammals and how these cells affect functions of the embryonic and adult brain.https://elifesciences.org/articles/25932lymphaticsendothelial cellbrain |
| spellingShingle | Max van Lessen Shannon Shibata-Germanos Andreas van Impel Thomas A Hawkins Jason Rihel Stefan Schulte-Merker Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development eLife lymphatics endothelial cell brain |
| title | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| title_full | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| title_fullStr | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| title_full_unstemmed | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| title_short | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| title_sort | intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
| topic | lymphatics endothelial cell brain |
| url | https://elifesciences.org/articles/25932 |
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