Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application
Barred loach Nemacheilus fasciatus are unable to reproduce naturally in captivity due to the lack of environmental cues and gonadal maturation occurs slower than in the wild. To optimize breeding procedures for this fish species, we determined whether hormone application via the gills was an effecti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Airlangga, Faculty of Veterinary Medicine
2024-04-01
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| Series: | Jurnal Medik Veteriner |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/49768 |
| _version_ | 1826906038370041856 |
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| author | Darmawan Setia Budi Buyung Raga Ardiyan Dani Bagus Rahmawan Josie South Arif Habib Fasya Akhmad Taufiq Mukti |
| author_facet | Darmawan Setia Budi Buyung Raga Ardiyan Dani Bagus Rahmawan Josie South Arif Habib Fasya Akhmad Taufiq Mukti |
| author_sort | Darmawan Setia Budi |
| collection | DOAJ |
| description | Barred loach Nemacheilus fasciatus are unable to reproduce naturally in captivity due to the lack of environmental cues and gonadal maturation occurs slower than in the wild. To optimize breeding procedures for this fish species, we determined whether hormone application via the gills was an effective process and assessed hormone dosage treatments. Oodev™ was used to induce gonadal maturation and Ovaprim™ was used to induce spawning via a topical gill approach. Multiple maturation parameters such as gonadal-somatic index (GSI) and hepatosomatic index (HSI), fecundity, and egg diameter; and spawning parameters such as latency period, egg produce, fertilization rate (FR), hatching rate (HR), survival rate (SR) were recorded and compared between the hormone dosage treatments and control treatment. Both the Oodev™ gonadal maturation induction and Ovaprim™ spawning induction were effectively applied to barred loach via topical gill application. Gonadal maturation parameters were positively correlated with dosage and all were significantly different. Fry survival rate was not different between doses. The optimal dose of Oodev™ (0.75 µL/g fish) resulted in male GSI of 5.334 ± 0.320%, female GSI of 15.501 ± 0.675%, male HSI of 0.416 ± 0.023%, female HSI of 1.670 ± 0.104%, egg fecundity 4584.20 ± 493.216 eggs, egg diameter 0.964 ± 0.0151 mm. Ovaprim™ optimal dosage (3.00 µL/g female and 1.50 µL/g male) was resulted latency period 11.05 ± 0.52 hours, egg produced 3504.83 ± 358.57, FR 96.77 ± 0.88%, HR 83.62 ± 3.78%, and SR 91.44 ± 2.53%. |
| first_indexed | 2024-04-24T16:02:04Z |
| format | Article |
| id | doaj.art-c4ec9e9c2498448f8bc03864c3627ee4 |
| institution | Directory Open Access Journal |
| issn | 2615-7497 2581-012X |
| language | English |
| last_indexed | 2025-02-17T08:40:23Z |
| publishDate | 2024-04-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj.art-c4ec9e9c2498448f8bc03864c3627ee42025-01-02T21:31:14ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-04-017112313310.20473/jmv.vol7.iss1.2024.123-13347915Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone ApplicationDarmawan Setia Budi0https://orcid.org/0000-0001-9508-1437Buyung Raga Ardiyan1Dani Bagus Rahmawan2Josie South3https://orcid.org/0000-0002-6339-4225Arif Habib Fasya4https://orcid.org/0000-0002-7588-8839Akhmad Taufiq Mukti5https://orcid.org/0000-0002-1649-5090Study Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaStudy Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaStudy Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaSchool of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United KingdomStudy Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, IndonesiaBarred loach Nemacheilus fasciatus are unable to reproduce naturally in captivity due to the lack of environmental cues and gonadal maturation occurs slower than in the wild. To optimize breeding procedures for this fish species, we determined whether hormone application via the gills was an effective process and assessed hormone dosage treatments. Oodev™ was used to induce gonadal maturation and Ovaprim™ was used to induce spawning via a topical gill approach. Multiple maturation parameters such as gonadal-somatic index (GSI) and hepatosomatic index (HSI), fecundity, and egg diameter; and spawning parameters such as latency period, egg produce, fertilization rate (FR), hatching rate (HR), survival rate (SR) were recorded and compared between the hormone dosage treatments and control treatment. Both the Oodev™ gonadal maturation induction and Ovaprim™ spawning induction were effectively applied to barred loach via topical gill application. Gonadal maturation parameters were positively correlated with dosage and all were significantly different. Fry survival rate was not different between doses. The optimal dose of Oodev™ (0.75 µL/g fish) resulted in male GSI of 5.334 ± 0.320%, female GSI of 15.501 ± 0.675%, male HSI of 0.416 ± 0.023%, female HSI of 1.670 ± 0.104%, egg fecundity 4584.20 ± 493.216 eggs, egg diameter 0.964 ± 0.0151 mm. Ovaprim™ optimal dosage (3.00 µL/g female and 1.50 µL/g male) was resulted latency period 11.05 ± 0.52 hours, egg produced 3504.83 ± 358.57, FR 96.77 ± 0.88%, HR 83.62 ± 3.78%, and SR 91.44 ± 2.53%.https://e-journal.unair.ac.id/JMV/article/view/49768breedingcaptivityfish speciesnatural habitatwild |
| spellingShingle | Darmawan Setia Budi Buyung Raga Ardiyan Dani Bagus Rahmawan Josie South Arif Habib Fasya Akhmad Taufiq Mukti Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application Jurnal Medik Veteriner breeding captivity fish species natural habitat wild |
| title | Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application |
| title_full | Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application |
| title_fullStr | Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application |
| title_full_unstemmed | Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application |
| title_short | Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application |
| title_sort | gonadal maturation and spawning of barred loach nemacheilus fasciatus induced by topical gill hormone application |
| topic | breeding captivity fish species natural habitat wild |
| url | https://e-journal.unair.ac.id/JMV/article/view/49768 |
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