Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer
<b>Background</b>: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precurs...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6054 |
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| author | Marina Dudea-Simon Dan Mihu Laura Ancuta Pop Razvan Ciortea Andrei Mihai Malutan Doru Diculescu Cristina Alexandra Ciocan Roxana Maria Cojocneanu Vasile Simon Carmen Bucuri Radu Mocan-Hognogi Cornelia Braicu Ioana Berindan-Neagoe |
| author_facet | Marina Dudea-Simon Dan Mihu Laura Ancuta Pop Razvan Ciortea Andrei Mihai Malutan Doru Diculescu Cristina Alexandra Ciocan Roxana Maria Cojocneanu Vasile Simon Carmen Bucuri Radu Mocan-Hognogi Cornelia Braicu Ioana Berindan-Neagoe |
| author_sort | Marina Dudea-Simon |
| collection | DOAJ |
| description | <b>Background</b>: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. <b>Methods</b>: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. <b>Results</b>: We observed higher expression levels for <i>MEG3, DAPK1</i>, <i>MLH1</i> and <i>MALAT1</i> in CIN samples than in normal samples, whereas <i>TIMP3</i> and <i>SOX1</i> had lower expression levels. For cancer samples, <i>DAPK1</i>, <i>MLH1</i> and <i>MALAT1</i> had higher expression, and <i>MEG3</i>, <i>TIMP3</i> and <i>SOX1</i> had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only <i>MEG3</i> gene showed a statistically significant difference. The expression of <i>miR-205-5p</i> was lower in both CIN and cancer samples compared to normal samples. <b>Conclusion:</b> Decreased <i>MEG3</i> expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of <i>TIMP3</i>, <i>SOX1</i>, <i>MLH1</i>, <i>MALAT1</i> and <i>miR-205-5p</i> could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations. |
| first_indexed | 2024-03-10T01:16:28Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:16:28Z |
| publishDate | 2022-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4ee69286b0e4997a7a257494fd796272023-11-23T14:08:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311605410.3390/ijms23116054Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical CancerMarina Dudea-Simon0Dan Mihu1Laura Ancuta Pop2Razvan Ciortea3Andrei Mihai Malutan4Doru Diculescu5Cristina Alexandra Ciocan6Roxana Maria Cojocneanu7Vasile Simon8Carmen Bucuri9Radu Mocan-Hognogi10Cornelia Braicu11Ioana Berindan-Neagoe122nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaUrology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, Romania<b>Background</b>: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. <b>Methods</b>: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. <b>Results</b>: We observed higher expression levels for <i>MEG3, DAPK1</i>, <i>MLH1</i> and <i>MALAT1</i> in CIN samples than in normal samples, whereas <i>TIMP3</i> and <i>SOX1</i> had lower expression levels. For cancer samples, <i>DAPK1</i>, <i>MLH1</i> and <i>MALAT1</i> had higher expression, and <i>MEG3</i>, <i>TIMP3</i> and <i>SOX1</i> had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only <i>MEG3</i> gene showed a statistically significant difference. The expression of <i>miR-205-5p</i> was lower in both CIN and cancer samples compared to normal samples. <b>Conclusion:</b> Decreased <i>MEG3</i> expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of <i>TIMP3</i>, <i>SOX1</i>, <i>MLH1</i>, <i>MALAT1</i> and <i>miR-205-5p</i> could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.https://www.mdpi.com/1422-0067/23/11/6054cervical cancercervical intraepithelial neoplasialong noncoding RNAsmicroRNA |
| spellingShingle | Marina Dudea-Simon Dan Mihu Laura Ancuta Pop Razvan Ciortea Andrei Mihai Malutan Doru Diculescu Cristina Alexandra Ciocan Roxana Maria Cojocneanu Vasile Simon Carmen Bucuri Radu Mocan-Hognogi Cornelia Braicu Ioana Berindan-Neagoe Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer International Journal of Molecular Sciences cervical cancer cervical intraepithelial neoplasia long noncoding RNAs microRNA |
| title | Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer |
| title_full | Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer |
| title_fullStr | Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer |
| title_full_unstemmed | Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer |
| title_short | Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer |
| title_sort | alteration of gene and mirna expression in cervical intraepithelial neoplasia and cervical cancer |
| topic | cervical cancer cervical intraepithelial neoplasia long noncoding RNAs microRNA |
| url | https://www.mdpi.com/1422-0067/23/11/6054 |
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