Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence
Vaginal and cervical canal bacteria are associated with women’s health and pregnancy outcomes. Here, we compared their composition and characteristics in 37 reproductive-aged Chinese women including 24 pregnant women with cervical incompetence (vaginal and cervical canal bacteria formed Groups A and...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.986326/full |
| _version_ | 1811208390571982848 |
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| author | Meiguo Sun Huiwu Geng Jingjing Bai Jiahui Feng Na Xu Yunlong Liu Xiaoying Liu Xiaoying Liu Gang Liu |
| author_facet | Meiguo Sun Huiwu Geng Jingjing Bai Jiahui Feng Na Xu Yunlong Liu Xiaoying Liu Xiaoying Liu Gang Liu |
| author_sort | Meiguo Sun |
| collection | DOAJ |
| description | Vaginal and cervical canal bacteria are associated with women’s health and pregnancy outcomes. Here, we compared their composition and characteristics in 37 reproductive-aged Chinese women including 24 pregnant women with cervical incompetence (vaginal and cervical canal bacteria formed Groups A and B, respectively) and 13 healthy pregnant women (vaginal and cervical canal bacteria formed Groups C and D, respectively) using high-throughput sequencing of the V4 region of 16S rRNA gene. The results of alpha and beta diversity analysis, respectively, indicated no statistical differences between Groups A and B (p = 0.32, 0.06), nor Groups B and D (p = 0.69, 0.74); however, differences were found between Groups C and D (p = 0.02, 0.01) and between Groups A and C (p = 0.04, 0.02). PLS-DA analysis showed that the individuals from each group were irregularly distributed according to their clade. Lactobacillus, Bifidobacterium and Ureaplasma were the dominant genera in all groups. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSts) analysis identified 31 Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs associated with the bacterial communities from the four groups, including membrane transport, folding, sorting and degradation, xenobiotics biodegradation and metabolism, and nucleotide metabolism. We further determined relationships between pregnancy outcomes (Apgar scores) and certain bacterial species. A significant positive correlation was found between Apgar scores and Actinomyces neuii and Anoxybacillus flavithermus in the vagina and cervical canal of pregnant women with cervical incompetence while Bacteroides plebeius, Bifidobacterium pseudopodium and Staphylococcus petrasii in the cervical canal displayed negative correlations with Apgar scores. Moreover, Clostridium fimetarium, Methanobacterium congolense, Pseudomonas chlororaphis, and Psychrobacter nivimaris in the vagina were negatively correlated with Apgar scores. These bacteria may serve as potential biomarkers, however, additional research is warranted to verify their role in clinical outcomes. |
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| id | doaj.art-c4f08d864e49434f91befd3abbb6d8a6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T04:20:26Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-c4f08d864e49434f91befd3abbb6d8a62022-12-22T03:48:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.986326986326Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetenceMeiguo Sun0Huiwu Geng1Jingjing Bai2Jiahui Feng3Na Xu4Yunlong Liu5Xiaoying Liu6Xiaoying Liu7Gang Liu8Department of Obstertrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, Anhui,ChinaDepartment of Obstertrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, Anhui,ChinaSchool of Life Sciences, Anhui Medical University, Hefei, Anhui,ChinaDepartment of Obstertrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, Anhui,ChinaTranslational Research Institute of Henan Provincial People’s Hospital and People’s Hospital of Zhengzhou University, Henan International Joint Laboratory of Non-coding RNA and Metabolism in Cancer, Henan Provincial Key Laboratory of Long Non-coding RNA and Cancer Metabolism, Zhengzhou, Henan, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, Anhui,ChinaVaginal and cervical canal bacteria are associated with women’s health and pregnancy outcomes. Here, we compared their composition and characteristics in 37 reproductive-aged Chinese women including 24 pregnant women with cervical incompetence (vaginal and cervical canal bacteria formed Groups A and B, respectively) and 13 healthy pregnant women (vaginal and cervical canal bacteria formed Groups C and D, respectively) using high-throughput sequencing of the V4 region of 16S rRNA gene. The results of alpha and beta diversity analysis, respectively, indicated no statistical differences between Groups A and B (p = 0.32, 0.06), nor Groups B and D (p = 0.69, 0.74); however, differences were found between Groups C and D (p = 0.02, 0.01) and between Groups A and C (p = 0.04, 0.02). PLS-DA analysis showed that the individuals from each group were irregularly distributed according to their clade. Lactobacillus, Bifidobacterium and Ureaplasma were the dominant genera in all groups. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSts) analysis identified 31 Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs associated with the bacterial communities from the four groups, including membrane transport, folding, sorting and degradation, xenobiotics biodegradation and metabolism, and nucleotide metabolism. We further determined relationships between pregnancy outcomes (Apgar scores) and certain bacterial species. A significant positive correlation was found between Apgar scores and Actinomyces neuii and Anoxybacillus flavithermus in the vagina and cervical canal of pregnant women with cervical incompetence while Bacteroides plebeius, Bifidobacterium pseudopodium and Staphylococcus petrasii in the cervical canal displayed negative correlations with Apgar scores. Moreover, Clostridium fimetarium, Methanobacterium congolense, Pseudomonas chlororaphis, and Psychrobacter nivimaris in the vagina were negatively correlated with Apgar scores. These bacteria may serve as potential biomarkers, however, additional research is warranted to verify their role in clinical outcomes.https://www.frontiersin.org/articles/10.3389/fmicb.2022.986326/fullcervical incompetencebacteriabiomarkerOTUsvagina and cervical canal |
| spellingShingle | Meiguo Sun Huiwu Geng Jingjing Bai Jiahui Feng Na Xu Yunlong Liu Xiaoying Liu Xiaoying Liu Gang Liu Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence Frontiers in Microbiology cervical incompetence bacteria biomarker OTUs vagina and cervical canal |
| title | Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| title_full | Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| title_fullStr | Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| title_full_unstemmed | Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| title_short | Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| title_sort | characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence |
| topic | cervical incompetence bacteria biomarker OTUs vagina and cervical canal |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.986326/full |
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