Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice
The COVID-19 pandemic caused by the SARS-CoV-2 infection induced lung inflammation characterized by cytokine storm and fulminant immune response of both resident and migrated immune cells, accelerating alveolar damage. In this work we identified members of the matrix metalloprotease (MMPs) family as...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1627 |
| _version_ | 1797407529569878016 |
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| author | Hila Gutman Moshe Aftalion Sharon Melamed Boaz Politi Reinat Nevo Sapir Havusha-Laufer Hagit Achdout David Gur Tomer Israely Shlomit Dachir Emanuelle Mamroud Irit Sagi Yaron Vagima |
| author_facet | Hila Gutman Moshe Aftalion Sharon Melamed Boaz Politi Reinat Nevo Sapir Havusha-Laufer Hagit Achdout David Gur Tomer Israely Shlomit Dachir Emanuelle Mamroud Irit Sagi Yaron Vagima |
| author_sort | Hila Gutman |
| collection | DOAJ |
| description | The COVID-19 pandemic caused by the SARS-CoV-2 infection induced lung inflammation characterized by cytokine storm and fulminant immune response of both resident and migrated immune cells, accelerating alveolar damage. In this work we identified members of the matrix metalloprotease (MMPs) family associated with lung extra-cellular matrix (ECM) destruction using K18-hACE2-transgenic mice (K18-hACE2) infected intranasally with SARS-CoV-2. Five days post infection, the lungs exhibited overall alveolar damage of epithelial cells and massive leukocytes infiltration. A substantial pulmonary increase in MMP8, MMP9, and MMP14 in the lungs post SARS-CoV-2 infection was associated with degradation of ECM components including collagen, laminin, and proteoglycans. The process of tissue damage and ECM degradation during SARS-CoV-2 lung infection is suggested to be associated with activity of members of the MMPs family, which in turn may be used as a therapeutic intervention. |
| first_indexed | 2024-03-09T03:42:49Z |
| format | Article |
| id | doaj.art-c4f0aa4bf09e4facaa63f1efdc0040de |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:42:49Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c4f0aa4bf09e4facaa63f1efdc0040de2023-12-03T14:38:38ZengMDPI AGViruses1999-49152022-07-01148162710.3390/v14081627Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 MiceHila Gutman0Moshe Aftalion1Sharon Melamed2Boaz Politi3Reinat Nevo4Sapir Havusha-Laufer5Hagit Achdout6David Gur7Tomer Israely8Shlomit Dachir9Emanuelle Mamroud10Irit Sagi11Yaron Vagima12Israel Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelDepartment of Biomolecular Sciences, Weizmann Institute of Science, Herzel 234, Rehovot P.O. Box 26, IsraelDepartment of Biological Regulation, Weizmann Institute of Science, Herzel 234, Rehovot P.O. Box 26, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelDepartment of Biological Regulation, Weizmann Institute of Science, Herzel 234, Rehovot P.O. Box 26, IsraelIsrael Institute for Biological Research, Ness Ziona P.O. Box 19, IsraelThe COVID-19 pandemic caused by the SARS-CoV-2 infection induced lung inflammation characterized by cytokine storm and fulminant immune response of both resident and migrated immune cells, accelerating alveolar damage. In this work we identified members of the matrix metalloprotease (MMPs) family associated with lung extra-cellular matrix (ECM) destruction using K18-hACE2-transgenic mice (K18-hACE2) infected intranasally with SARS-CoV-2. Five days post infection, the lungs exhibited overall alveolar damage of epithelial cells and massive leukocytes infiltration. A substantial pulmonary increase in MMP8, MMP9, and MMP14 in the lungs post SARS-CoV-2 infection was associated with degradation of ECM components including collagen, laminin, and proteoglycans. The process of tissue damage and ECM degradation during SARS-CoV-2 lung infection is suggested to be associated with activity of members of the MMPs family, which in turn may be used as a therapeutic intervention.https://www.mdpi.com/1999-4915/14/8/1627COVID-19extra-cellular matrix (ECM)K18-hACE2matrix-metalloproteases (MMP)SARS-CoV-2 |
| spellingShingle | Hila Gutman Moshe Aftalion Sharon Melamed Boaz Politi Reinat Nevo Sapir Havusha-Laufer Hagit Achdout David Gur Tomer Israely Shlomit Dachir Emanuelle Mamroud Irit Sagi Yaron Vagima Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice Viruses COVID-19 extra-cellular matrix (ECM) K18-hACE2 matrix-metalloproteases (MMP) SARS-CoV-2 |
| title | Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice |
| title_full | Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice |
| title_fullStr | Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice |
| title_full_unstemmed | Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice |
| title_short | Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice |
| title_sort | matrix metalloproteinases expression is associated with sars cov 2 induced lung pathology and extracellular matrix remodeling in k18 hace2 mice |
| topic | COVID-19 extra-cellular matrix (ECM) K18-hACE2 matrix-metalloproteases (MMP) SARS-CoV-2 |
| url | https://www.mdpi.com/1999-4915/14/8/1627 |
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