The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens
Antimicrobial peptides (AMPs) constitute an essential part of the plant immune system. They are regarded as alternatives to conventional antibiotics and pesticides. In this study, we have identified the γ-core motifs, which are associated with antimicrobial activity, in 18 AMPs from grasses and assa...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8383 |
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| author | Marina P. Slezina Ekaterina A. Istomina Ekaterina V. Kulakovskaya Tatyana V. Korostyleva Tatyana I. Odintsova |
| author_facet | Marina P. Slezina Ekaterina A. Istomina Ekaterina V. Kulakovskaya Tatyana V. Korostyleva Tatyana I. Odintsova |
| author_sort | Marina P. Slezina |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) constitute an essential part of the plant immune system. They are regarded as alternatives to conventional antibiotics and pesticides. In this study, we have identified the γ-core motifs, which are associated with antimicrobial activity, in 18 AMPs from grasses and assayed their antimicrobial properties against nine pathogens, including yeasts affecting humans, as well as plant pathogenic bacteria and fungi. All the tested peptides displayed antimicrobial properties. We discovered a number of short AMP-derived peptides with high antimicrobial activity both against human and plant pathogens. For the first time, antimicrobial activity was revealed in the peptides designed from the 4-Cys-containing defensin-like peptides, whose role in plant immunity has remained unknown, as well as the knottin-like peptide and the C-terminal prodomain of the thionin, which points to the direct involvement of these peptides in defense mechanisms. Studies of the mode of action of the eight most active γ-core motif peptides on yeast cells using staining with propidium iodide showed that all of them induced membrane permeabilization leading to cell lysis. In addition to identification of the antimicrobial determinants in plant AMPs, this work provides short candidate peptide molecules for the development of novel drugs effective against opportunistic fungal infections and biopesticides to control plant pathogens. |
| first_indexed | 2024-03-09T12:34:13Z |
| format | Article |
| id | doaj.art-c4f59577f0bf41308c2f53a85b5709bb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:34:13Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c4f59577f0bf41308c2f53a85b5709bb2023-11-30T22:26:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315838310.3390/ijms23158383The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant PathogensMarina P. Slezina0Ekaterina A. Istomina1Ekaterina V. Kulakovskaya2Tatyana V. Korostyleva3Tatyana I. Odintsova4Vavilov Institute of General Genetics RAS, 119333 Moscow, RussiaVavilov Institute of General Genetics RAS, 119333 Moscow, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, 142290 Pushchino, RussiaVavilov Institute of General Genetics RAS, 119333 Moscow, RussiaVavilov Institute of General Genetics RAS, 119333 Moscow, RussiaAntimicrobial peptides (AMPs) constitute an essential part of the plant immune system. They are regarded as alternatives to conventional antibiotics and pesticides. In this study, we have identified the γ-core motifs, which are associated with antimicrobial activity, in 18 AMPs from grasses and assayed their antimicrobial properties against nine pathogens, including yeasts affecting humans, as well as plant pathogenic bacteria and fungi. All the tested peptides displayed antimicrobial properties. We discovered a number of short AMP-derived peptides with high antimicrobial activity both against human and plant pathogens. For the first time, antimicrobial activity was revealed in the peptides designed from the 4-Cys-containing defensin-like peptides, whose role in plant immunity has remained unknown, as well as the knottin-like peptide and the C-terminal prodomain of the thionin, which points to the direct involvement of these peptides in defense mechanisms. Studies of the mode of action of the eight most active γ-core motif peptides on yeast cells using staining with propidium iodide showed that all of them induced membrane permeabilization leading to cell lysis. In addition to identification of the antimicrobial determinants in plant AMPs, this work provides short candidate peptide molecules for the development of novel drugs effective against opportunistic fungal infections and biopesticides to control plant pathogens.https://www.mdpi.com/1422-0067/23/15/8383plant antimicrobial peptidesγ-coreantimicrobial activitynovel antimicrobials<i>Fusarium</i> speciesplant pathogenic bacteria |
| spellingShingle | Marina P. Slezina Ekaterina A. Istomina Ekaterina V. Kulakovskaya Tatyana V. Korostyleva Tatyana I. Odintsova The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens International Journal of Molecular Sciences plant antimicrobial peptides γ-core antimicrobial activity novel antimicrobials <i>Fusarium</i> species plant pathogenic bacteria |
| title | The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens |
| title_full | The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens |
| title_fullStr | The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens |
| title_full_unstemmed | The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens |
| title_short | The γ-Core Motif Peptides of AMPs from Grasses Display Inhibitory Activity against Human and Plant Pathogens |
| title_sort | γ core motif peptides of amps from grasses display inhibitory activity against human and plant pathogens |
| topic | plant antimicrobial peptides γ-core antimicrobial activity novel antimicrobials <i>Fusarium</i> species plant pathogenic bacteria |
| url | https://www.mdpi.com/1422-0067/23/15/8383 |
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