Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i>
Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/1/7 |
| _version_ | 1797358350321582080 |
|---|---|
| author | Prashanth Tamizhselvan Sharmila Madhavan Christian Constan-Aguilar Eman Ryad Elrefaay Jie Liu Aleš Pěnčík Ondřej Novák Albert Cairó Mónika Hrtyan Markus Geisler Vanesa Beatriz Tognetti |
| author_facet | Prashanth Tamizhselvan Sharmila Madhavan Christian Constan-Aguilar Eman Ryad Elrefaay Jie Liu Aleš Pěnčík Ondřej Novák Albert Cairó Mónika Hrtyan Markus Geisler Vanesa Beatriz Tognetti |
| author_sort | Prashanth Tamizhselvan |
| collection | DOAJ |
| description | Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator auxin and for sensing environmental stress, but their role in intracellular auxin homeostasis, vital for plant growth and survival in changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 and ABCB29, which export auxin across the chloroplast envelope to the cytosol in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis pathway in <i>Arabidopsis thaliana</i> chloroplasts. The overexpression of ABCB28 and ABCB29 influenced stomatal regulation and resulted in significantly improved water use efficiency and survival rates during salt and drought stresses. Our results suggest that chloroplast auxin production and transport contribute to stomata regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate photosynthesis and auxin signals and as such hold great potential to improve the adaptation potential of crops to environmental cues. |
| first_indexed | 2024-03-08T14:59:41Z |
| format | Article |
| id | doaj.art-c4f637cf2d664ea7be757a665f38e42d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T14:59:41Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c4f637cf2d664ea7be757a665f38e42d2024-01-10T15:05:52ZengMDPI AGPlants2223-77472023-12-01131710.3390/plants13010007Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i>Prashanth Tamizhselvan0Sharmila Madhavan1Christian Constan-Aguilar2Eman Ryad Elrefaay3Jie Liu4Aleš Pěnčík5Ondřej Novák6Albert Cairó7Mónika Hrtyan8Markus Geisler9Vanesa Beatriz Tognetti10Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicDepartment of Biology, University of Fribourg, CH-1700 Fribourg, SwitzerlandLaboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of Sciences, & Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech RepublicLaboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of Sciences, & Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech RepublicMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicDepartment of Biology, University of Fribourg, CH-1700 Fribourg, SwitzerlandMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech RepublicPhotosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator auxin and for sensing environmental stress, but their role in intracellular auxin homeostasis, vital for plant growth and survival in changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 and ABCB29, which export auxin across the chloroplast envelope to the cytosol in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis pathway in <i>Arabidopsis thaliana</i> chloroplasts. The overexpression of ABCB28 and ABCB29 influenced stomatal regulation and resulted in significantly improved water use efficiency and survival rates during salt and drought stresses. Our results suggest that chloroplast auxin production and transport contribute to stomata regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate photosynthesis and auxin signals and as such hold great potential to improve the adaptation potential of crops to environmental cues.https://www.mdpi.com/2223-7747/13/1/7auxinhormone transportABC transporterstressphotosynthesisdrought |
| spellingShingle | Prashanth Tamizhselvan Sharmila Madhavan Christian Constan-Aguilar Eman Ryad Elrefaay Jie Liu Aleš Pěnčík Ondřej Novák Albert Cairó Mónika Hrtyan Markus Geisler Vanesa Beatriz Tognetti Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> Plants auxin hormone transport ABC transporter stress photosynthesis drought |
| title | Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> |
| title_full | Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> |
| title_fullStr | Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> |
| title_full_unstemmed | Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> |
| title_short | Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in <i>Arabidopsis</i> |
| title_sort | chloroplast auxin efflux mediated by abcb28 and abcb29 fine tunes salt and drought stress responses in i arabidopsis i |
| topic | auxin hormone transport ABC transporter stress photosynthesis drought |
| url | https://www.mdpi.com/2223-7747/13/1/7 |
| work_keys_str_mv | AT prashanthtamizhselvan chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT sharmilamadhavan chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT christianconstanaguilar chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT emanryadelrefaay chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT jieliu chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT alespencik chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT ondrejnovak chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT albertcairo chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT monikahrtyan chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT markusgeisler chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi AT vanesabeatriztognetti chloroplastauxineffluxmediatedbyabcb28andabcb29finetunessaltanddroughtstressresponsesiniarabidopsisi |