Interference of electron transfer chain inhibitors in bioelectrochemical systems
Substances inhibiting specific proteins involved in cellular electron transfer chains are used in biochemical research to investigate intracellular electron transfer routes and to redirect the electron fluxes. This also provides an in vivo approach to investigating the extracellular electron transfe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Electrochemistry Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248123001017 |
| _version_ | 1797790081746993152 |
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| author | Hans Schneider Bin Lai Jens O. Krömer |
| author_facet | Hans Schneider Bin Lai Jens O. Krömer |
| author_sort | Hans Schneider |
| collection | DOAJ |
| description | Substances inhibiting specific proteins involved in cellular electron transfer chains are used in biochemical research to investigate intracellular electron transfer routes and to redirect the electron fluxes. This also provides an in vivo approach to investigating the extracellular electron transfer (EET) mechanisms within and across biological membranes in bioelectrochemical research. However, the applicability of these specific inhibitors in electrochemical systems remains to be validated, particularly when aiming for (semi-)quantitative evaluation of the contribution of specific redox proteins to the EET. Here we conducted a systematic analysis of commonly used inhibitors and discovered several of them to be electrochemically active and thus capable of interfering with measurements in a bioelectrochemical reactor system in abiotic experiments. We also observed effects in vivo using a biophotovoltaics reactor with Synechocystis sp. PCC 6803 as a model system. |
| first_indexed | 2024-03-13T01:59:41Z |
| format | Article |
| id | doaj.art-c4f905dbd3e94e538da1ba3d7c55e199 |
| institution | Directory Open Access Journal |
| issn | 1388-2481 |
| language | English |
| last_indexed | 2024-03-13T01:59:41Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj.art-c4f905dbd3e94e538da1ba3d7c55e1992023-07-02T04:16:23ZengElsevierElectrochemistry Communications1388-24812023-07-01152107527Interference of electron transfer chain inhibitors in bioelectrochemical systemsHans Schneider0Bin Lai1Jens O. Krömer2Systems Biotechnology Group, Department Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, GermanyBMBF Junior Research Group Biophotovoltaics, Department Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, Germany; Corresponding author.Systems Biotechnology Group, Department Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, GermanySubstances inhibiting specific proteins involved in cellular electron transfer chains are used in biochemical research to investigate intracellular electron transfer routes and to redirect the electron fluxes. This also provides an in vivo approach to investigating the extracellular electron transfer (EET) mechanisms within and across biological membranes in bioelectrochemical research. However, the applicability of these specific inhibitors in electrochemical systems remains to be validated, particularly when aiming for (semi-)quantitative evaluation of the contribution of specific redox proteins to the EET. Here we conducted a systematic analysis of commonly used inhibitors and discovered several of them to be electrochemically active and thus capable of interfering with measurements in a bioelectrochemical reactor system in abiotic experiments. We also observed effects in vivo using a biophotovoltaics reactor with Synechocystis sp. PCC 6803 as a model system.http://www.sciencedirect.com/science/article/pii/S1388248123001017Extracellular electron transferInhibitorPhotosystemRespiration |
| spellingShingle | Hans Schneider Bin Lai Jens O. Krömer Interference of electron transfer chain inhibitors in bioelectrochemical systems Electrochemistry Communications Extracellular electron transfer Inhibitor Photosystem Respiration |
| title | Interference of electron transfer chain inhibitors in bioelectrochemical systems |
| title_full | Interference of electron transfer chain inhibitors in bioelectrochemical systems |
| title_fullStr | Interference of electron transfer chain inhibitors in bioelectrochemical systems |
| title_full_unstemmed | Interference of electron transfer chain inhibitors in bioelectrochemical systems |
| title_short | Interference of electron transfer chain inhibitors in bioelectrochemical systems |
| title_sort | interference of electron transfer chain inhibitors in bioelectrochemical systems |
| topic | Extracellular electron transfer Inhibitor Photosystem Respiration |
| url | http://www.sciencedirect.com/science/article/pii/S1388248123001017 |
| work_keys_str_mv | AT hansschneider interferenceofelectrontransferchaininhibitorsinbioelectrochemicalsystems AT binlai interferenceofelectrontransferchaininhibitorsinbioelectrochemicalsystems AT jensokromer interferenceofelectrontransferchaininhibitorsinbioelectrochemicalsystems |