Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India.
Bovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with r...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0232093 |
| _version_ | 1818677955481567232 |
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| author | Naveen Kumar Yogesh Chander Thachamvally Riyesh Nitin Khandelwal Ram Kumar Harish Kumar Bhupendra N Tripathi Sanjay Barua |
| author_facet | Naveen Kumar Yogesh Chander Thachamvally Riyesh Nitin Khandelwal Ram Kumar Harish Kumar Bhupendra N Tripathi Sanjay Barua |
| author_sort | Naveen Kumar |
| collection | DOAJ |
| description | Bovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with rhinotracheitis and abortion, BoHV5 causes encephalitis in cattle. BoHV5 outbreaks are sporadic and mainly restricted to the South American countries. We report BoHV5 infection for the first time from aborted cattle in India. Based on the characteristic cytopathic effects in MDBK cells, amplification of the viral genome by PCR, differential PCR for BoHV1/BoHV5, nucleotide sequencing and restriction endonuclease patterns, identity of the virus was confirmed as BoHV5 subtype A. Serum samples from the aborted cattle strongly neutralized both BoHV1 and BoHV5 suggesting an active viral infection in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the isolated virus clustered with BoHV5 strains and showed highest similarity with the Brazilian BoHV5 strains. |
| first_indexed | 2024-12-17T09:07:35Z |
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| id | doaj.art-c4fb81d9e78d426f8638e23b442a274b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T09:07:35Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c4fb81d9e78d426f8638e23b442a274b2022-12-21T21:55:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01154e023209310.1371/journal.pone.0232093Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India.Naveen KumarYogesh ChanderThachamvally RiyeshNitin KhandelwalRam KumarHarish KumarBhupendra N TripathiSanjay BaruaBovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with rhinotracheitis and abortion, BoHV5 causes encephalitis in cattle. BoHV5 outbreaks are sporadic and mainly restricted to the South American countries. We report BoHV5 infection for the first time from aborted cattle in India. Based on the characteristic cytopathic effects in MDBK cells, amplification of the viral genome by PCR, differential PCR for BoHV1/BoHV5, nucleotide sequencing and restriction endonuclease patterns, identity of the virus was confirmed as BoHV5 subtype A. Serum samples from the aborted cattle strongly neutralized both BoHV1 and BoHV5 suggesting an active viral infection in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the isolated virus clustered with BoHV5 strains and showed highest similarity with the Brazilian BoHV5 strains.https://doi.org/10.1371/journal.pone.0232093 |
| spellingShingle | Naveen Kumar Yogesh Chander Thachamvally Riyesh Nitin Khandelwal Ram Kumar Harish Kumar Bhupendra N Tripathi Sanjay Barua Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. PLoS ONE |
| title | Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. |
| title_full | Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. |
| title_fullStr | Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. |
| title_full_unstemmed | Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. |
| title_short | Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India. |
| title_sort | isolation and characterization of bovine herpes virus 5 bohv5 from cattle in india |
| url | https://doi.org/10.1371/journal.pone.0232093 |
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