Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus
Background: Rice sheath blight (caused by Rhizoctonia solani) and tobacco mosaic virus are very important plant diseases, causing a huge loss in global crop production. Paenibacillus kribbensis PS04 is a broad-spectrum biocontrol agent, used for controlling these diseases. Previously, extracellular...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345820300336 |
| _version_ | 1818483475000328192 |
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| author | Shu Canwei Hu Xiaoyun Nauman Ahmed Wang Shiqi Zhou Erxun Liao Meide |
| author_facet | Shu Canwei Hu Xiaoyun Nauman Ahmed Wang Shiqi Zhou Erxun Liao Meide |
| author_sort | Shu Canwei |
| collection | DOAJ |
| description | Background: Rice sheath blight (caused by Rhizoctonia solani) and tobacco mosaic virus are very important plant diseases, causing a huge loss in global crop production. Paenibacillus kribbensis PS04 is a broad-spectrum biocontrol agent, used for controlling these diseases. Previously, extracellular polysaccharides (EPS) from P. kribbensis PS04 had been purified and their structure was inferred to be fructosan. This study aimed to evaluate the effects of exogenous EPS treatment on plant–pathogen interactions. Results: Plant defense genes such as phenylalanine ammonia-lyase, catalase, chitinase, allene oxide synthase, and PR1a proteins were significantly induced by exogenous EPS treatment. Moreover, subsequent challenge of EPS-pretreated plants with the pathogens (R. solani or tobacco mosaic virus) resulted in higher expression of defense-associated genes. Increased activities of defense-associated enzymes, total phenols, and flavonoids were also observed in EPS pretreated plants. The contents of malondialdehyde in plants, which act as indicator of lipid peroxidation, were reduced by EPS treatment. Conclusions: This study comprehensively showed that EPS produced from P. kribbensis PS04 enhances disease resistance in plants by the activation of defense-associated genes as well as through the enhancement of activities of defense-related enzymes. How to cite: Canwei S, Xiaoyun H, Ahmed N, et al. Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus. Electron J Biotechnol 2020;47. https://doi.org/10.1016/j.ejbt.2020.07.002 |
| first_indexed | 2024-12-10T15:42:28Z |
| format | Article |
| id | doaj.art-c4fe4305e57e47dcb98b6f3e8962205b |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-10T15:42:28Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-c4fe4305e57e47dcb98b6f3e8962205b2022-12-22T01:43:04ZengElsevierElectronic Journal of Biotechnology0717-34582020-09-01474350Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virusShu Canwei0Hu Xiaoyun1Nauman Ahmed2Wang Shiqi3Zhou Erxun4Liao Meide5Integrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaIntegrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaIntegrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaIntegrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaIntegrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCorresponding author.; Integrative Microbiology Research Centre/National Engineering Research Center of Plant Space Breeding, College of Agriculture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaBackground: Rice sheath blight (caused by Rhizoctonia solani) and tobacco mosaic virus are very important plant diseases, causing a huge loss in global crop production. Paenibacillus kribbensis PS04 is a broad-spectrum biocontrol agent, used for controlling these diseases. Previously, extracellular polysaccharides (EPS) from P. kribbensis PS04 had been purified and their structure was inferred to be fructosan. This study aimed to evaluate the effects of exogenous EPS treatment on plant–pathogen interactions. Results: Plant defense genes such as phenylalanine ammonia-lyase, catalase, chitinase, allene oxide synthase, and PR1a proteins were significantly induced by exogenous EPS treatment. Moreover, subsequent challenge of EPS-pretreated plants with the pathogens (R. solani or tobacco mosaic virus) resulted in higher expression of defense-associated genes. Increased activities of defense-associated enzymes, total phenols, and flavonoids were also observed in EPS pretreated plants. The contents of malondialdehyde in plants, which act as indicator of lipid peroxidation, were reduced by EPS treatment. Conclusions: This study comprehensively showed that EPS produced from P. kribbensis PS04 enhances disease resistance in plants by the activation of defense-associated genes as well as through the enhancement of activities of defense-related enzymes. How to cite: Canwei S, Xiaoyun H, Ahmed N, et al. Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus. Electron J Biotechnol 2020;47. https://doi.org/10.1016/j.ejbt.2020.07.002http://www.sciencedirect.com/science/article/pii/S0717345820300336BiocontrolDisease resistanceExtracellular polysaccharidesFructosanPaenibacillusPlant defense genes |
| spellingShingle | Shu Canwei Hu Xiaoyun Nauman Ahmed Wang Shiqi Zhou Erxun Liao Meide Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus Electronic Journal of Biotechnology Biocontrol Disease resistance Extracellular polysaccharides Fructosan Paenibacillus Plant defense genes |
| title | Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus |
| title_full | Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus |
| title_fullStr | Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus |
| title_full_unstemmed | Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus |
| title_short | Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus |
| title_sort | fructosan form paenibacillus kribbensis ps04 enhance disease resistance against rhizoctonia solani and tobacco mosaic virus |
| topic | Biocontrol Disease resistance Extracellular polysaccharides Fructosan Paenibacillus Plant defense genes |
| url | http://www.sciencedirect.com/science/article/pii/S0717345820300336 |
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