Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran
Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention. Methods: In this study, 70 imipenem-resistant Acinet...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | https://jmb.tums.ac.ir/index.php/jmb/article/view/46 |
| _version_ | 1811266725657706496 |
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| author | Neda Noori Jalil Vandyosefi Faeze Sabet Sara Ashrafi Kiarash Ghazvini |
| author_facet | Neda Noori Jalil Vandyosefi Faeze Sabet Sara Ashrafi Kiarash Ghazvini |
| author_sort | Neda Noori |
| collection | DOAJ |
| description | Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention.
Methods: In this study, 70 imipenem-resistant Acinetobacter strains were isolated from health care associated infections. These isolates were screened for detection of metallo-beta-lactamase (MBL) using inhibitor potentiated disk diffusion tests with thylenediaminetetraacetic acid (EDTA). PCR was designed for detection of bla vim and bla imp-1 using specific primers.
Results: Among these 70 strains, 50 strains appeared to produce metallo-beta-lactamase. Three isolates were detected by PCR to carry metallo-beta-lactamase gene bla vim, but bla imp-1 gene was not detected.
Conclusion: These findings suggest that in our area other genetic elements are responsible for resistance against metallo-beta-lactams. |
| first_indexed | 2024-04-12T20:48:28Z |
| format | Article |
| id | doaj.art-c4feb9177a18462d80b6150d4f3c3d9d |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-04-12T20:48:28Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-c4feb9177a18462d80b6150d4f3c3d9d2022-12-22T03:17:13ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-10-0123-444Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of IranNeda Noori0Jalil Vandyosefi1Faeze Sabet2Sara Ashrafi3Kiarash Ghazvini4Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, IR Iran.Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, IR Iran.Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran.Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran.Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran.Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention. Methods: In this study, 70 imipenem-resistant Acinetobacter strains were isolated from health care associated infections. These isolates were screened for detection of metallo-beta-lactamase (MBL) using inhibitor potentiated disk diffusion tests with thylenediaminetetraacetic acid (EDTA). PCR was designed for detection of bla vim and bla imp-1 using specific primers. Results: Among these 70 strains, 50 strains appeared to produce metallo-beta-lactamase. Three isolates were detected by PCR to carry metallo-beta-lactamase gene bla vim, but bla imp-1 gene was not detected. Conclusion: These findings suggest that in our area other genetic elements are responsible for resistance against metallo-beta-lactams.https://jmb.tums.ac.ir/index.php/jmb/article/view/46AcinetobacterCarbapenemaseIran |
| spellingShingle | Neda Noori Jalil Vandyosefi Faeze Sabet Sara Ashrafi Kiarash Ghazvini Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran Journal of Medical Bacteriology Acinetobacter Carbapenemase Iran |
| title | Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran |
| title_full | Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran |
| title_fullStr | Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran |
| title_full_unstemmed | Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran |
| title_short | Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran |
| title_sort | frequency of imp 1 and vim genes among metallo beta lactamase producing acinetobacter spp isolated from health care associated infections innortheast of iran |
| topic | Acinetobacter Carbapenemase Iran |
| url | https://jmb.tums.ac.ir/index.php/jmb/article/view/46 |
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