Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses
The aim of this study was to evaluate the bond strength, microleakage, cytotoxicity, cell migration and fluoride ion release over time from a resin-modified glass-ionomer cement (RMGIC) enriched with bioactive glasses (BAGs) and a nanohybrid restorative polymer resin agent used as adhesion material...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Symmetry |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-8994/13/3/494 |
| _version_ | 1797540996646436864 |
|---|---|
| author | Ascensión Vicente Francisco Javier Rodríguez-Lozano Yolanda Martínez-Beneyto María Jaimez Julia Guerrero-Gironés Antonio J. Ortiz-Ruiz |
| author_facet | Ascensión Vicente Francisco Javier Rodríguez-Lozano Yolanda Martínez-Beneyto María Jaimez Julia Guerrero-Gironés Antonio J. Ortiz-Ruiz |
| author_sort | Ascensión Vicente |
| collection | DOAJ |
| description | The aim of this study was to evaluate the bond strength, microleakage, cytotoxicity, cell migration and fluoride ion release over time from a resin-modified glass-ionomer cement (RMGIC) enriched with bioactive glasses (BAGs) and a nanohybrid restorative polymer resin agent used as adhesion material in the cemented brackets. One hundred and twenty bovine lower incisors were divided into three groups: (Transbond Plus Self Etching Primer (TSEP)/Transbond XT (TXT), TSEP/ACTIVA, orthophosphoric acid gel/ACTIVA) and brackets were bonded. A bond strength test and microleakage test were applied. A fluoride release test was applied after 60 days for the TXT and ACTIVA group. To evaluate cytotoxicity and cell migration, a cell viability and scratch migration assay were done for each group. <i>p</i> values < 0.05 were considered significant. Regarding bond strength and microleakage test, no significant differences were found between TSEP/TXT and TSEP/ACTIVA. At 6.4 pH, ACTIVA showed a higher degree of fluoride ion release, which increased with acid pH (3.5), with a maximum fluoride secretion at 30 days. MTT assay revealed that TXT reduces the viability of gingival cells with significant differences (<i>p</i> < 0.001) compared to the untreated cells (control group). ACTIVA provides optimal adhesive and microfiltration properties, releases substantial amounts of fluoride ions in both acid and neutral media, and its biocompatibility is greater than that of traditional composite resin adhesive systems. |
| first_indexed | 2024-03-10T13:08:59Z |
| format | Article |
| id | doaj.art-c4ff1499841e43079de47c97ab4e9697 |
| institution | Directory Open Access Journal |
| issn | 2073-8994 |
| language | English |
| last_indexed | 2024-03-10T13:08:59Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Symmetry |
| spelling | doaj.art-c4ff1499841e43079de47c97ab4e96972023-11-21T10:55:31ZengMDPI AGSymmetry2073-89942021-03-0113349410.3390/sym13030494Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive GlassesAscensión Vicente0Francisco Javier Rodríguez-Lozano1Yolanda Martínez-Beneyto2María Jaimez3Julia Guerrero-Gironés4Antonio J. Ortiz-Ruiz5Department of Orthodontics, Faculty of Medicine-Dentistry, University of Murcia, 30100 Murcia, SpainDepartment of Gerodontology & Special Care Dentistry, Faculty of Medicine-Dentistry, IMIB-Arrixaca, University of Murcia, 30100 Murcia, SpainDepartment of Preventive and Community Dentistry, Faculty of Medicine-Dentistry, University of Murcia, 30100 Murcia, SpainDepartment of Orthodontics, Faculty of Medicine-Dentistry, University of Murcia, 30100 Murcia, SpainDepartment of Dermatology, Stomatology and Radiology, Faculty of Medicine-Dentistry, University of Murcia, 30100 Murcia, SpainDepartment of Integrated Pediatric Dentistry, Faculty of Medicine-Dentistry, University of Murcia, 30100 Murcia, SpainThe aim of this study was to evaluate the bond strength, microleakage, cytotoxicity, cell migration and fluoride ion release over time from a resin-modified glass-ionomer cement (RMGIC) enriched with bioactive glasses (BAGs) and a nanohybrid restorative polymer resin agent used as adhesion material in the cemented brackets. One hundred and twenty bovine lower incisors were divided into three groups: (Transbond Plus Self Etching Primer (TSEP)/Transbond XT (TXT), TSEP/ACTIVA, orthophosphoric acid gel/ACTIVA) and brackets were bonded. A bond strength test and microleakage test were applied. A fluoride release test was applied after 60 days for the TXT and ACTIVA group. To evaluate cytotoxicity and cell migration, a cell viability and scratch migration assay were done for each group. <i>p</i> values < 0.05 were considered significant. Regarding bond strength and microleakage test, no significant differences were found between TSEP/TXT and TSEP/ACTIVA. At 6.4 pH, ACTIVA showed a higher degree of fluoride ion release, which increased with acid pH (3.5), with a maximum fluoride secretion at 30 days. MTT assay revealed that TXT reduces the viability of gingival cells with significant differences (<i>p</i> < 0.001) compared to the untreated cells (control group). ACTIVA provides optimal adhesive and microfiltration properties, releases substantial amounts of fluoride ions in both acid and neutral media, and its biocompatibility is greater than that of traditional composite resin adhesive systems.https://www.mdpi.com/2073-8994/13/3/494fluoride releasemicroleakageenamel bond strengthcell viabilitycell migrationBAG |
| spellingShingle | Ascensión Vicente Francisco Javier Rodríguez-Lozano Yolanda Martínez-Beneyto María Jaimez Julia Guerrero-Gironés Antonio J. Ortiz-Ruiz Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses Symmetry fluoride release microleakage enamel bond strength cell viability cell migration BAG |
| title | Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses |
| title_full | Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses |
| title_fullStr | Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses |
| title_full_unstemmed | Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses |
| title_short | Biophysical and Fluoride Release Properties of a Resin Modified Glass Ionomer Cement Enriched with Bioactive Glasses |
| title_sort | biophysical and fluoride release properties of a resin modified glass ionomer cement enriched with bioactive glasses |
| topic | fluoride release microleakage enamel bond strength cell viability cell migration BAG |
| url | https://www.mdpi.com/2073-8994/13/3/494 |
| work_keys_str_mv | AT ascensionvicente biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses AT franciscojavierrodriguezlozano biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses AT yolandamartinezbeneyto biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses AT mariajaimez biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses AT juliaguerrerogirones biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses AT antoniojortizruiz biophysicalandfluoridereleasepropertiesofaresinmodifiedglassionomercementenrichedwithbioactiveglasses |