HOTAIR expression and prognostic impact in acute myeloid leukemia patients
Abstract Background Acute myeloid leukemia (AML) is a disorder characterized by a rapid onset of symptoms attributable to bone marrow failure due to clonal proliferation of primitive hematopoietic stem cells or progenitor cells. Epigenetic abnormalities play an important role in the development and...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-05-01
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| Series: | Egyptian Journal of Medical Human Genetics |
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| Online Access: | https://doi.org/10.1186/s43042-021-00147-y |
| _version_ | 1827327275122556928 |
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| author | Rawda Ahmed Alaaeldin Ahmed Mohamed Saad Amany Ahmed Osman Mona Fathey Abdel Fattah Hassan Shereen Abdel Monem Ibrahim Yasmin Nabil El-Sakhawy |
| author_facet | Rawda Ahmed Alaaeldin Ahmed Mohamed Saad Amany Ahmed Osman Mona Fathey Abdel Fattah Hassan Shereen Abdel Monem Ibrahim Yasmin Nabil El-Sakhawy |
| author_sort | Rawda Ahmed Alaaeldin Ahmed Mohamed Saad |
| collection | DOAJ |
| description | Abstract Background Acute myeloid leukemia (AML) is a disorder characterized by a rapid onset of symptoms attributable to bone marrow failure due to clonal proliferation of primitive hematopoietic stem cells or progenitor cells. Epigenetic abnormalities play an important role in the development and progression of acute leukemia. Long non-coding ribonucleic acid (lncRNA) plays an important role in epigenetic regulation. Homeobox (Hox) transcript antisense intergenic RNA (HOTAIR) is a lncRNA which has been determined to be a negative prognostic indicator in various solid-tumor patients. However, its role in hematopoietic tumors as AML is to be assessed. This study aimed at measuring lncRNA HOTAIR expression level on bone marrow (BM) mononuclear cells in newly diagnosed AML patients and correlating its expression with their outcome and different prognostic variables. This provides new prospective for a novel marker involved in development and progression of AML which can be used as a diagnostic marker and a target of therapy. The current study included 65 subjects divided into 35 newly diagnosed AML adult patients (before initiation of chemotherapy) and 30 non-leukemic adult patients who are candidates for BM aspiration for causes other than hematological malignancies as immune thrombocytopenic purpura and hypersplenism as controls. HOTAIR expression was measured on BM mononuclear cells by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results HOTAIR expression was found to be significantly upregulated in AML patients (probability (p) value = 0.000) and it can be used as a diagnostic biomarker of AML as confirmed by a significant difference between cases and controls using receiver operating characteristic curve (ROC) analysis. However, it was not significantly correlated with event free survival (EFS) or prognostic variables. Conclusion This study showed that the expression of HOTAIR is upregulated in de novo AML patients and can be used as a diagnostic marker. However, highly expressed HOTAIR is not associated with poor prognosis. |
| first_indexed | 2024-03-07T14:57:48Z |
| format | Article |
| id | doaj.art-c502983aa2484b83a047184a999ed1b9 |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-03-07T14:57:48Z |
| publishDate | 2021-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-c502983aa2484b83a047184a999ed1b92024-03-05T19:19:30ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412021-05-0122111110.1186/s43042-021-00147-yHOTAIR expression and prognostic impact in acute myeloid leukemia patientsRawda Ahmed Alaaeldin Ahmed Mohamed Saad0Amany Ahmed Osman1Mona Fathey Abdel Fattah Hassan2Shereen Abdel Monem Ibrahim3Yasmin Nabil El-Sakhawy4Clinical Pathology Department, Faculty of Medicine, Ain Shams UniversityClinical Pathology Department, Faculty of Medicine, Ain Shams UniversityClinical Pathology Department, Faculty of Medicine, Ain Shams UniversityClinical Pathology Department, Faculty of Medicine, Ain Shams UniversityClinical Pathology Department, Faculty of Medicine, Ain Shams UniversityAbstract Background Acute myeloid leukemia (AML) is a disorder characterized by a rapid onset of symptoms attributable to bone marrow failure due to clonal proliferation of primitive hematopoietic stem cells or progenitor cells. Epigenetic abnormalities play an important role in the development and progression of acute leukemia. Long non-coding ribonucleic acid (lncRNA) plays an important role in epigenetic regulation. Homeobox (Hox) transcript antisense intergenic RNA (HOTAIR) is a lncRNA which has been determined to be a negative prognostic indicator in various solid-tumor patients. However, its role in hematopoietic tumors as AML is to be assessed. This study aimed at measuring lncRNA HOTAIR expression level on bone marrow (BM) mononuclear cells in newly diagnosed AML patients and correlating its expression with their outcome and different prognostic variables. This provides new prospective for a novel marker involved in development and progression of AML which can be used as a diagnostic marker and a target of therapy. The current study included 65 subjects divided into 35 newly diagnosed AML adult patients (before initiation of chemotherapy) and 30 non-leukemic adult patients who are candidates for BM aspiration for causes other than hematological malignancies as immune thrombocytopenic purpura and hypersplenism as controls. HOTAIR expression was measured on BM mononuclear cells by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results HOTAIR expression was found to be significantly upregulated in AML patients (probability (p) value = 0.000) and it can be used as a diagnostic biomarker of AML as confirmed by a significant difference between cases and controls using receiver operating characteristic curve (ROC) analysis. However, it was not significantly correlated with event free survival (EFS) or prognostic variables. Conclusion This study showed that the expression of HOTAIR is upregulated in de novo AML patients and can be used as a diagnostic marker. However, highly expressed HOTAIR is not associated with poor prognosis.https://doi.org/10.1186/s43042-021-00147-yAMLEpigeneticslncRNAHOTAIR |
| spellingShingle | Rawda Ahmed Alaaeldin Ahmed Mohamed Saad Amany Ahmed Osman Mona Fathey Abdel Fattah Hassan Shereen Abdel Monem Ibrahim Yasmin Nabil El-Sakhawy HOTAIR expression and prognostic impact in acute myeloid leukemia patients Egyptian Journal of Medical Human Genetics AML Epigenetics lncRNA HOTAIR |
| title | HOTAIR expression and prognostic impact in acute myeloid leukemia patients |
| title_full | HOTAIR expression and prognostic impact in acute myeloid leukemia patients |
| title_fullStr | HOTAIR expression and prognostic impact in acute myeloid leukemia patients |
| title_full_unstemmed | HOTAIR expression and prognostic impact in acute myeloid leukemia patients |
| title_short | HOTAIR expression and prognostic impact in acute myeloid leukemia patients |
| title_sort | hotair expression and prognostic impact in acute myeloid leukemia patients |
| topic | AML Epigenetics lncRNA HOTAIR |
| url | https://doi.org/10.1186/s43042-021-00147-y |
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