OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS
This paper presents the optimization of a rapid, inexpensive, reliable and selective isocratic high performance liquid chromatographic (HPLC) method for the simultaneous determination of caffeine and taurine in energy drinks with two common detectors in series: evaporating light scattering detector...
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2018-03-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201801&vol=1&aid=4687 |
| _version_ | 1817994451244548096 |
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| author | RALUCA-IOANA [CHIRITA] TAMPU ADRIANA FINARU CLAIRE ELFAKIR |
| author_facet | RALUCA-IOANA [CHIRITA] TAMPU ADRIANA FINARU CLAIRE ELFAKIR |
| author_sort | RALUCA-IOANA [CHIRITA] TAMPU |
| collection | DOAJ |
| description | This paper presents the optimization of a rapid, inexpensive, reliable and selective isocratic high performance liquid chromatographic (HPLC) method for the simultaneous determination of caffeine and taurine in energy drinks with two common detectors in series: evaporating light scattering detector (ELSD) and an ultraviolet (UV) detector. Satisfactory analysis results were obtained on an Astec apHera NH2 column using methanol/water (30:70 v/v) as mobile phase. The optimized method was used for the analysis of commercial energy drinks containing large amounts of carbohydrates (100 g·L-1) and considerably lower amounts of taurine and caffeine (4 and 0.6 g·L-1, respectively). The advantages of this method consist of its lack of preliminary samples treatment and also the fact that basic LC instrumentation was employed. |
| first_indexed | 2024-04-14T01:52:53Z |
| format | Article |
| id | doaj.art-c504ab181a244a13b6ce45fb680fa755 |
| institution | Directory Open Access Journal |
| issn | 1582-540X 1582-540X |
| language | English |
| last_indexed | 2024-04-14T01:52:53Z |
| publishDate | 2018-03-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj.art-c504ab181a244a13b6ce45fb680fa7552022-12-22T02:19:18ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2018-03-011912332OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKSRALUCA-IOANA [CHIRITA] TAMPU0ADRIANA FINARU1CLAIRE ELFAKIR21. " Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania 2. Institut de Chimie Organique et Analytique CNRS FR 2708 UMR 6005, University of Orléans, F-45067 Orléans, France "Vasile Alecsandri” University of Bacau, Calea Marasesti, 157, Bacau-600115, Romania Institut de Chimie Organique et Analytique CNRS FR 2708 UMR 6005, University of Orléans, F-45067 Orléans, France This paper presents the optimization of a rapid, inexpensive, reliable and selective isocratic high performance liquid chromatographic (HPLC) method for the simultaneous determination of caffeine and taurine in energy drinks with two common detectors in series: evaporating light scattering detector (ELSD) and an ultraviolet (UV) detector. Satisfactory analysis results were obtained on an Astec apHera NH2 column using methanol/water (30:70 v/v) as mobile phase. The optimized method was used for the analysis of commercial energy drinks containing large amounts of carbohydrates (100 g·L-1) and considerably lower amounts of taurine and caffeine (4 and 0.6 g·L-1, respectively). The advantages of this method consist of its lack of preliminary samples treatment and also the fact that basic LC instrumentation was employed.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201801&vol=1&aid=4687CaffeineEnergy DrinkHPLCTaurine |
| spellingShingle | RALUCA-IOANA [CHIRITA] TAMPU ADRIANA FINARU CLAIRE ELFAKIR OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Caffeine Energy Drink HPLC Taurine |
| title | OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS |
| title_full | OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS |
| title_fullStr | OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS |
| title_full_unstemmed | OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS |
| title_short | OPTIMIZATION OF A HPLC ANALYSIS METHOD FOR TAURINE AND CAFFEINE IN ENERGY DRINKS |
| title_sort | optimization of a hplc analysis method for taurine and caffeine in energy drinks |
| topic | Caffeine Energy Drink HPLC Taurine |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201801&vol=1&aid=4687 |
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